Title:
TRANSFORMANT AND PROCESS FOR PRODUCTION THEREOF, AND PROCESS FOR PRODUCTION OF LACTIC ACID
Document Type and Number:
WIPO Patent Application WO/2011/021629
Kind Code:
A1
Abstract:
A transformant which is produced using Schizosaccharomyces pombe as a host and has a lactate dehydrogenase gene integrated therein, wherein a part of a gene encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or deactivated.
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Inventors:
HARA FUTOSHI (JP)
TOHDA HIDEKI (JP)
HAMA YUKO
TOHDA HIDEKI (JP)
HAMA YUKO
Application Number:
PCT/JP2010/063888
Publication Date:
February 24, 2011
Filing Date:
August 17, 2010
Export Citation:
Assignee:
ASAHI GLASS CO LTD (JP)
HARA FUTOSHI (JP)
TOHDA HIDEKI (JP)
HAMA CHIHIRO
HARA FUTOSHI (JP)
TOHDA HIDEKI (JP)
HAMA CHIHIRO
International Classes:
C12N15/09; C12N1/19; C12P7/56
Domestic Patent References:
| WO2005052174A2 | 2005-06-09 | |||
| WO1999014335A1 | 1999-03-25 | |||
| WO2007063919A1 | 2007-06-07 | |||
| WO2002101038A1 | 2002-12-19 | |||
| WO2007015470A1 | 2007-02-08 | |||
| WO1995009914A1 | 1995-04-13 | |||
| WO1999023223A1 | 1999-05-14 | |||
| WO2007026617A1 | 2007-03-08 | |||
| WO1996023890A1 | 1996-08-08 |
Foreign References:
| JP2006006271A | 2006-01-12 | |||
| JP2001204464A | 2001-07-31 | |||
| JP2008048726A | 2008-03-06 | |||
| JPH0515380A | 1993-01-26 | |||
| JPH10234375A | 1998-09-08 | |||
| JP2000262284A | 2000-09-26 | |||
| JP2005198612A | 2005-07-28 | |||
| JPH07163373A | 1995-06-27 |
Other References:
"Abstracts of the Annual Meeting of the Society for Biotechnology, Japan, 2008", vol. 60TH, article FUTOSHI HARA ET AL.: "Lactate production using Schizosaccharomyces pombe", pages: 135, XP008151367
BIANCHI, M.M. ET AL.: "Efficient homolactic fermentation by Kluyveromyces lactis strains defective in pyruvate utilization and transformed with the heterologous LDH gene", APPL. ENVIRON. MICROBIOL., vol. 67, no. 12, 2001, pages 5621 - 5625, XP002236026
ISHIDA, N. ET AL.: "The effect of pyruvate decarboxylase gene knockout in Saccharomyces cerevisiae on L-lactic acid production.", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 70, no. 5, 2006, pages 1148 - 1153, XP008129166
IKUSHIMA, S. ET AL.: "Genetic engineering of Candida utilis yeast for efficient production of L-lactic acid.", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 73, no. 8, 7 August 2009 (2009-08-07), pages 1818 - 1824, XP008151373
WOOD, V. ET AL.: "The genome sequence of Schizosaccharomyces pombe.", NATURE, vol. 415, no. 6874, 2002, pages 871 - 880, XP002306285
"Annual Meeting of the Molecular Biology Society of Japan Program Koen Yoshishu", vol. 26TH, 2003, article MASAO SAKURAI ET AL.: "Bunretsu Kobo no Alcohol Dehydrogenase Idenshi Hakai Kabu ni Okeru Totaisha no Kaiseki", pages: 609, XP008151363
"Abstracts of the Annual Meeting of the Society for Biotechnology, Japan", vol. 61TH, 25 August 2009, article FUTOSHI HARA ET AL.: "Lactate production using Schizosaccharomyces pombe", pages: 190, XP008151368
"Annual Meeting of the Molecular Biology Society of Japan Program Koen Yoshishu", vol. 32TH, 20 November 2009, article FUTOSHI HARA ET AL.: "Bunretsu Kobo o Mochiita Muchuwa deno L-Nyusan Seisan ni Okeru Taisha Seigyo", pages: 263, XP008151364
See also references of EP 2468860A4
NUCLEIC ACIDS RES., vol. 34, 2006, pages E11
"Methods for Yeast Molecular Genetics Experimentation", 1996, GAKKAI SHUPPAN CENTER
PCR METHODS APPL., vol. 2, 1992, pages 28 - 33
TSUJIBO H; TIANO HF; LI SS-L: "Nucleotide sequence of the cDNA and an intronless pseudogene for human lactate dehydrogenase-A isozyme", EUR. J. BIOCHEM., vol. 147, 1985, pages 9 - 15, XP003010998, DOI: doi:10.1111/j.1432-1033.1985.tb08711.x
NATHAN J. BOWEN ET AL.: "Retrotransposons and Their Recognition of pol II Promoters: A Comprehensive Survey of the Transposable Elements From the Complete Genome Sequence of Schizosaccharomyces pombe", GENOME RES., vol. 13, 2003, pages 1984 - 1997
UICHI LINO, ARC 010, GENOTYPE: H- LEUL-32 URA4-D18
NUCLEIC ACIDS RES., vol. 34, 2006, pages EL 1
BAHLER ET AL., YEAST, vol. 14, 1998, pages 943 - 951
IDIRIS ET AL., YEAST, vol. 23, 2006, pages 83 - 99
TSUJIBO ET AL., EUR. J. BIOCHEM, vol. 147, 1985, pages 9 - 15
OKAYAMA, H.; BERG, P.: "A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells", MOL. CELL. BIOL., vol. 3, 1983, pages 280 - 289, XP008020929
OKAZAKI ET AL., NUCLEIC ACIDS RES., vol. 18, 1990, pages 6485 - 6489
BIANCHI, M.M. ET AL.: "Efficient homolactic fermentation by Kluyveromyces lactis strains defective in pyruvate utilization and transformed with the heterologous LDH gene", APPL. ENVIRON. MICROBIOL., vol. 67, no. 12, 2001, pages 5621 - 5625, XP002236026
ISHIDA, N. ET AL.: "The effect of pyruvate decarboxylase gene knockout in Saccharomyces cerevisiae on L-lactic acid production.", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 70, no. 5, 2006, pages 1148 - 1153, XP008129166
IKUSHIMA, S. ET AL.: "Genetic engineering of Candida utilis yeast for efficient production of L-lactic acid.", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 73, no. 8, 7 August 2009 (2009-08-07), pages 1818 - 1824, XP008151373
WOOD, V. ET AL.: "The genome sequence of Schizosaccharomyces pombe.", NATURE, vol. 415, no. 6874, 2002, pages 871 - 880, XP002306285
"Annual Meeting of the Molecular Biology Society of Japan Program Koen Yoshishu", vol. 26TH, 2003, article MASAO SAKURAI ET AL.: "Bunretsu Kobo no Alcohol Dehydrogenase Idenshi Hakai Kabu ni Okeru Totaisha no Kaiseki", pages: 609, XP008151363
"Abstracts of the Annual Meeting of the Society for Biotechnology, Japan", vol. 61TH, 25 August 2009, article FUTOSHI HARA ET AL.: "Lactate production using Schizosaccharomyces pombe", pages: 190, XP008151368
"Annual Meeting of the Molecular Biology Society of Japan Program Koen Yoshishu", vol. 32TH, 20 November 2009, article FUTOSHI HARA ET AL.: "Bunretsu Kobo o Mochiita Muchuwa deno L-Nyusan Seisan ni Okeru Taisha Seigyo", pages: 263, XP008151364
See also references of EP 2468860A4
NUCLEIC ACIDS RES., vol. 34, 2006, pages E11
"Methods for Yeast Molecular Genetics Experimentation", 1996, GAKKAI SHUPPAN CENTER
PCR METHODS APPL., vol. 2, 1992, pages 28 - 33
TSUJIBO H; TIANO HF; LI SS-L: "Nucleotide sequence of the cDNA and an intronless pseudogene for human lactate dehydrogenase-A isozyme", EUR. J. BIOCHEM., vol. 147, 1985, pages 9 - 15, XP003010998, DOI: doi:10.1111/j.1432-1033.1985.tb08711.x
NATHAN J. BOWEN ET AL.: "Retrotransposons and Their Recognition of pol II Promoters: A Comprehensive Survey of the Transposable Elements From the Complete Genome Sequence of Schizosaccharomyces pombe", GENOME RES., vol. 13, 2003, pages 1984 - 1997
UICHI LINO, ARC 010, GENOTYPE: H- LEUL-32 URA4-D18
NUCLEIC ACIDS RES., vol. 34, 2006, pages EL 1
BAHLER ET AL., YEAST, vol. 14, 1998, pages 943 - 951
IDIRIS ET AL., YEAST, vol. 23, 2006, pages 83 - 99
TSUJIBO ET AL., EUR. J. BIOCHEM, vol. 147, 1985, pages 9 - 15
OKAYAMA, H.; BERG, P.: "A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells", MOL. CELL. BIOL., vol. 3, 1983, pages 280 - 289, XP008020929
OKAZAKI ET AL., NUCLEIC ACIDS RES., vol. 18, 1990, pages 6485 - 6489
Attorney, Agent or Firm:
OGURI Shohei et al. (JP)
Shohei Oguri (JP)
Shohei Oguri (JP)
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