Title:
TRANSGENIC PLANT RESISTANT TO MYCOTOXINS AND METHODS
Document Type and Number:
WIPO Patent Application WO/2000/060061
Kind Code:
A2
Abstract:
The invention relates to a plant cell comprising a heterologous polynucleotide encoding a gene product that is expressed in the plant cell wherein the gene product comprises trichothecene resistance activity
Inventors:
HOHN THOMAS M (US)
PETERS CHERYL (US)
SALMERON JOHN MANUEL (US)
REED JANET N (US)
DAWSON JOHN LUTHER (US)
PETERS CHERYL (US)
SALMERON JOHN MANUEL (US)
REED JANET N (US)
DAWSON JOHN LUTHER (US)
Application Number:
PCT/EP2000/002769
Publication Date:
October 12, 2000
Filing Date:
March 29, 2000
Export Citation:
Assignee:
NOVARTIS AG (CH)
NOVARTIS ERFIND VERWALT GMBH (AT)
HOHN THOMAS M (US)
PETERS CHERYL (US)
SALMERON JOHN MANUEL (US)
REED JANET N (US)
DAWSON JOHN LUTHER (US)
NOVARTIS ERFIND VERWALT GMBH (AT)
HOHN THOMAS M (US)
PETERS CHERYL (US)
SALMERON JOHN MANUEL (US)
REED JANET N (US)
DAWSON JOHN LUTHER (US)
International Classes:
C12N1/15; C12N5/10; C12N9/10; C12N15/09; C12N15/29; C12N15/54; C12N15/82; A01H5/00; C12R1/645; C12R1/91; (IPC1-7): C12N9/10; C12N15/54; C12N15/29; C12N15/82; C12N15/63; C12N5/04; A01H5/00
Domestic Patent References:
| WO1999009173A1 | 1999-02-25 |
Other References:
KIMURA MAKOTO ET AL: "The mystery of the trichothecene 3-O-acetyltransferase gene. Analysis of the region around Tri101 and characterization of its homologue form Fusarium sporotrichioides." FEBS LETTERS, vol. 435, no. 2-3, 1998, pages 163-168, XP000946098 ISSN: 0014-5793 cited in the application
KIMURA M ET AL: "Trichothecene 3-O-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins. Cloning and characterization of Tri101" JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 273, no. 3, 16 January 1998 (1998-01-16), pages 1654-1661, XP002125091 ISSN: 0021-9258 cited in the application
KIMURA MAKOTO ET AL: "Features of Tri101, the trichothecene 3-0-acetyltransferase gene, related to the self-defense mechanism in Fusarium graminearum." BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 62, no. 5, May 1998 (1998-05), pages 1033-1036, XP000946099 ISSN: 0916-8451 cited in the application
KIM ET AL: "RIBOSOMAL PROTEIN GENE EXPRESSION AND TRICHOTHECENE RESISTANCE IN ARABIDOPSIS THALIANA" DATABASE DISSABS AN 91:4157, XP002083007
DATABASE EMBL NUCLEOTIDE AND PROTEIN SEQUENCES, 28 May 1996 (1996-05-28), XP002147697 HINXTON, GB
DATABASE WPI Section Ch, Week 200024 Derwent Publications Ltd., London, GB; Class C06, AN 2000-274037 XP002147698 & JP 2000 032985 A (RIKAGAKU KENKYUSHO), 2 February 2000 (2000-02-02)
MCCORMICK SUSAN P ET AL: "Disruption of TRI101, the gene encoding trichothecene 3-O-acetyltransferase, from Fusarium sporotrichioides." APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 12, December 1999 (1999-12), pages 5252-5256, XP000938222 ISSN: 0099-2240 cited in the application
KIMURA M ET AL: "Trichothecene 3-O-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins. Cloning and characterization of Tri101" JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 273, no. 3, 16 January 1998 (1998-01-16), pages 1654-1661, XP002125091 ISSN: 0021-9258 cited in the application
KIMURA MAKOTO ET AL: "Features of Tri101, the trichothecene 3-0-acetyltransferase gene, related to the self-defense mechanism in Fusarium graminearum." BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 62, no. 5, May 1998 (1998-05), pages 1033-1036, XP000946099 ISSN: 0916-8451 cited in the application
KIM ET AL: "RIBOSOMAL PROTEIN GENE EXPRESSION AND TRICHOTHECENE RESISTANCE IN ARABIDOPSIS THALIANA" DATABASE DISSABS AN 91:4157, XP002083007
DATABASE EMBL NUCLEOTIDE AND PROTEIN SEQUENCES, 28 May 1996 (1996-05-28), XP002147697 HINXTON, GB
DATABASE WPI Section Ch, Week 200024 Derwent Publications Ltd., London, GB; Class C06, AN 2000-274037 XP002147698 & JP 2000 032985 A (RIKAGAKU KENKYUSHO), 2 February 2000 (2000-02-02)
MCCORMICK SUSAN P ET AL: "Disruption of TRI101, the gene encoding trichothecene 3-O-acetyltransferase, from Fusarium sporotrichioides." APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 12, December 1999 (1999-12), pages 5252-5256, XP000938222 ISSN: 0099-2240 cited in the application
Attorney, Agent or Firm:
Becker, Konrad (Syngenta Participations AG Intellectual
Property P.O. Box Basel, CH)
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Claims:
What is claimed is:
| 1. | A plant cell comprising a heterologous polynucleotide encoding a gene product that is expressed in the plant cell wherein the gene product has trichothecene resistance activity. |
| 2. | A plant comprising a plant cell of claim 1, wherein the plant is resistant to a trichothecene. |
| 3. | The plant according to claim 2, wherein the plant is resistant to a trichothecene comprising a C3 hydroxyl group. |
| 4. | The plant of claim 1, wherein the plant is resistant to a fungus that produces a trichothecene, preferably a trichothecene comprising a C3 hydroxyl group. |
| 5. | The plant according to claim 4, wherein the plant is resistant to Fusarium, preferably to Fusarium graminearum. |
| 6. | The plant of claim 2, wherein the heterologous polynucleotide is a microbial polynucleotide, preferably a yeast or fungal polynucleotide. |
| 7. | The plant of claim 6, wherein the fungal polynucleotide is a Fusarium polynucleotide, preferably is a Fusarium graminearum or Fusarium sporotrichioides polynucleotide. |
| 8. | The plant according to any one of claims 1 to 7, wherein the heterologous polynucleotide comprises a sequence substantially similar to SEQ ID NO: 1,5 or 7. |
| 9. | The plant according to claim 8, wherein the heterologous polynucleotide comprises the nucleic acid sequence of SEQ ID NO: 1,5 or 7. |
| 10. | The plant of claim 8 comprising a heterologous polynucleotide, which comprises a consecutive at least 80 base pair portion identical in sequence to a consecutive 80 base pair portion set forth in SEQ ID NO: 1,5 or 7, preferably a consecutive at least 50 base pair portion identical in sequence to a consecutive 50 base pair portion set forth in SEQ ID NO: 1,5 or 7, and more preferably a consecutive at least 21 base pair portion identical in sequence to a consecutive 21 base pair portion set forth in SEQ ID NO: 1,5 or 7, and most preferably a consecutive 18 base pair portion identical in sequence to a consecutive 18 base pair portion set forth in SEQ ID NO: 1,5 or 7. |
| 11. | The plant according to claim 10, wherein the heterologous polynucleotide comprises a consecutive 18 base pair portion identical in sequence to a consecutive 18 base pair portion set forth in SEQ ID NO: 1,5 or 7. |
| 12. | The plant of any one of claims 1 to 11, wherein the trichothecene is selected from the group consisting of T2 toxin, HT2 toxin, isotrichodermol, DAS, 3deacetylcalonectrin, 3,15dideacetylcalonectrin, scirpentriol, neosolaniol; type B: 15acetyideoxynivalenol, nivalenol, 4acetyinivalenol (fusarenoneX), 4,15diacetylnivalenol, 4,7,15 acetylnivalenol, and DON, but preferably DON or DAS. |
| 13. | The plant of claim 2 wherein the gene product is encoded by a polynucleotide according to any one of claims 1 and 6 to 9. |
| 14. | The plant of claim 13 wherein the gene product is a protein conferring trichothecene resistance to a plant, preferably a acetyltransferase, but especially a 3O acetyltransferase. |
| 15. | The plant according to claim 14, wherein the gene product is a polypeptide comprising a sequence substantially similar to SEQ ID NO: 2,6 or 8. |
| 16. | The plant according to any one of claims 2 to 15, wherein the plant is a wheat, maize, barley or rice plant. |
| 17. | A seed of the plant according to anyone of claims 2 to 15. |
| 18. | A method for producing a trichothecene resistant plant comprising the steps of: a) transforming a plant cell with a heterologous gene encoding a gene product, wherein the gene product increases resistance to a trichothecene; b) expressing the gene product at a biologically significant level c) regenerating the plant cell into a plant; and d) selecting a plant with increased resistance to a trichothecene. |
| 19. | The method of claim 18 further comprising the step of selecting a plant on which growth of fungus, wherein the fungus produces a trichothecene virulence factor, is reduced. |
| 20. | The method of claim 19, wherein the fungus is of the genus Fusarium. |
| 21. | A method of preventing mycotoxin contamination of a plant, including of a plant's seed, comprising growing the plant of any one of claims 2 to 16 or a plant produced by a method according to claims 18 to 20, preferably in an area with moderate to severe fungal infestation. |
| 22. | A method of claim 20, wherein the plant is a crop plant. |
| 23. | A method for reducing and/or preventing the growth of a fungus, preferably a fungus of the genus Fusarium, on a plant that produces a trichothecene, preferbably a trichothece comprising a C3 hydroxyl group, comprising growing the plant of any one of claims 2 to 16 or a plant produced by a method according to claims 18 to 20, preferably in an area with moderate to severe fungal infestation. |
| 24. | , The method of any one of claims 18 to 24, wherein the plant is a crop plant. |
| 25. | A method of producing a fungal resistant plant comprising (a) transforming a plant cell with a heterologous gene encoding a gene product, wherein the gene product increases resistance to a trichothecene; (b) expressing the gene product at a biologically significant level; (c) regenerating the plant cell into a plant; and (d) selecting a plant with increased resistance to a trichothecene; and (e) optionally, selfing or outcrossig the plant obtained in step (d). |
| 26. | The method of claim 25, wherein the fungus is of the genus Fusarium. |
| 27. | A method of producing seed wherein the plant grown from the seed is fungal resistant, comprising selfing or outcrossing the plant of any one of claims 2 to 16 or a plant produced by a method according to claims 25 to 26 and collecting the seed. |
| 28. | The method of claim 27, wherein the seed is resistant to fungi to the genus Fusarium. |
| 29. | A method of selecting transformed host cells, the method comprising: transforming a host cell with a nucleic acid construct encoding a trichothecene 30 acetyltransferase, growing the transformed host cell in the presence of a trichothecene. |
| 30. | The method of claim 29 wherein the host cell is a plant cell. |
| 31. | The method of claim 30, wherein the host cell is a microbial host cell. |
| 32. | The method of claim 31, wherein in the microbial host cell is a fungal host cell. |
| 33. | The method of claim 29, wherein the trichothecene is selected from the group consisting T2 toxin, HT2 toxin, isotrichodermol, DAS, 3deacetylcalonectrin, 3, 15 dideacetylcalonectrin, scirpentriol, neosolaniol; type B: 15acetyldeoxynivalenol, nivalenol, 4acetylnivalenol (fusarenoneX), 4,15diacetylnivalenol, 4,7,15acetyinivalenol, and DON. |
| 34. | A trichothecene resistant plant obtainable by the method of any one of claims 18 to 20. |
| 35. | A fungal resistant plant obtainable by the method of any one of claims 25 to 26. |
| 36. | A plant seed obtainable by the method of any one of claims 27 to 28. |
Description:
INTERNATIONAL SEARCH REPORT'" ApptionNo PCT/EP00/02769 C. (Continuatlon) DOCUMENTS CONSIDERED TO BE RELEVANT Category ° Citation of document, with indicabontwhere appropnate, of the relevant passages Relevant to claim No. X KIMURA M ET AL:"Trichothecene 1-36 3-0-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins. Cloning and characterization of Tri 101" JOURNAL OF BIOLOGICAL CHEMISTRY, US, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 273, no. 3, 16 January 1998 (1998-01-16), pages 1654-1661, XP002125091 ISSN: 0021-9258 cited in the application the whole document X KIMURA MAKOTO ET AL:"Features of Tri101, 1-36 the trichothecene 3-0-acetyltransferase gene, related to the self-defense mechanism in Fusarium graminearum." BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 62, no. 5, May 1998 (1998-05), pages 1033-1036,XP000946099 ISSN: 0916-8451 cited in the application the whole document X KIM ET AL:"RIBOSOMAL PROTEIN GENE 1 EXPRESSION AND TRICHOTHECENE RESISTANCE IN ARABIDOPSIS THALIANA" DATABASE DISSABS AN 91: 4157, XP002083007 PH. D. DISSERTATION, THE OHIO STATE UNIVERSITY, 1991, AVAILABLE FROM UMI ORDER NO. AAR9120676 X WO 99 09173 A (GLEDDIE STEPHEN C; HARRIS 1-36 LINDA J (CA); SIMMONDS JOHN A (CA); CANA) 25 February 1999 (1999-02-25) the whole document X DATABASE EMBL NUCLEOTIDE AND PROTEIN 8-11, SEQUENCES, 13-15 28 May 1996 (1996-05-28), XP002147697 HINXTON, GB AC = Z73168. S. cerevisiae chromosome XII reading frame ORF YLL063c abstract INTERNATIONAL SEARCH | Int@~ znal Appilcathn No PCT/EP 00/02769 C. (Continuatlon) DOCUMENTS CONSIDERED TO BE RELEVANT Category Citation of document, with indication, where appropiiate, of the relevant passages Relevant to daim No. P, X DATABASE WPI 8-11, Section Ch, Week 200024 13-15 Derwent Publications Ltd., London, GB; Class C06, AN 2000-274037 XP002147698 &JP 2000 032985 A (RIKAGAKU KENKYUSHO), 2 February 2000 (2000-02-02) abstract P, X MCCORMICK SUSAN P ET AL :"Disruption of 1-36 TRI101, the gene encoding trichothecene 3-0-acetyltransferase, from Fusarium sporotrichioides." APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 12, December 1999 (1999-12), pages 5252-5256, XP000938222 ISSN:0099-2240 cited in the application the whole document L INTERNATIONAL SEARCH REPORT Int nal Appllcation No lortnatbn on patent famfly members PCT/EP 00/02769 Patent document Publication Patent family Publication cited in search report date member (s) date WO 9909173 A 25-02-1999 US 6060646 A 09-05-2000 AU 8526398 A 08-03-1999 EP 1003867 A 31-05-2000 JP 2000032985 A 02-02-2000 NONE