Title:
TRIGGERED ASSEMBLY OF METAFLUOROPHORES
Document Type and Number:
WIPO Patent Application WO/2016/140726
Kind Code:
A3
Abstract:
Aspects of the present disclosure relate to systems, kits and methods that comprise a nucleic acid capture strand linked to a first dye molecule, a nucleic acid trigger strand longer than the capture strand and comprising (a) a capture domain that is complementary to the capture strand and (b) at least two concatenated domains, each of which comprises two subdomains, and a partially double-stranded nucleic acid comprising a single- stranded toehold domain having a nucleotide sequence complementary to one of the subdomains of the two subdomains of the concatenated domains, a double-stranded region linked to a second dye molecule and having a nucleotide sequence complementary to the other of the two subdomains of the concatenated domains, and a single- stranded hairpin loop having a nucleotide sequence that is complementary to the single-stranded toehold domain.
Inventors:
ONG LUVENA L (US)
ZHANG DAVID YU (US)
WEI DIMING (US)
JUNGMANN RALF (DE)
YIN PENG (US)
ZHANG DAVID YU (US)
WEI DIMING (US)
JUNGMANN RALF (DE)
YIN PENG (US)
Application Number:
PCT/US2015/065948
Publication Date:
October 27, 2016
Filing Date:
December 16, 2015
Export Citation:
Assignee:
HARVARD COLLEGE (US)
International Classes:
G01N21/64; C12Q1/68
Domestic Patent References:
| WO2012058488A1 | 2012-05-03 |
Foreign References:
| US20130261019A1 | 2013-10-03 |
Other References:
GIBRIEL, AAY.: "Options Available for Labelling Nucleic Acid Samples in DNA Microarray-based Detection Methods.", BRIEFINGS IN FUNCTIONAL GENOMICS., vol. 11, no. 4, 17 April 2012 (2012-04-17), pages 311 - 318, XP055325116
HEAD, SR ET AL.: "Library Construction for Next-generation Sequencing: Overviews and Challenges.", BIOTECHNIQUES., vol. 56, no. 2, 1 February 2014 (2014-02-01), pages 61 - 68, XP055108708
HEAD, SR ET AL.: "Library Construction for Next-generation Sequencing: Overviews and Challenges.", BIOTECHNIQUES., vol. 56, no. 2, 1 February 2014 (2014-02-01), pages 61 - 68, XP055108708
Attorney, Agent or Firm:
DIPIETRANTONIO, Heather, J. (Greenfield & Sacks P.C.,600 Atlantic Avenu, Boston MA, US)
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