Title:
USE OF alpha CP1, alpha CP2, AND HUR FOR MODULATING GENE EXPRESSION AND INDUCING ANGIOGENESIS
Document Type and Number:
WIPO Patent Application WO2003016343
Kind Code:
A3
Abstract:
The present invention describes methods for modulating gene expression, stabilizing VEGF mRNAs, for inducing angiogenesis, for treating various mammalian diseases including coronary and cardiac diseases and for identifying modulators of gene expression by using human proteins called alpha CP1, alpha CP2 and HuR. The invention also describes mRNA stabilizing elements and consensus sequences involved in binding of alpha CP1, alpha CP2 and HuR proteins to mRNAs.
Inventors:
GUY LOUIS-GEORGES (CA)
Application Number:
PCT/CA2002/001275
Publication Date:
September 25, 2003
Filing Date:
August 16, 2002
Export Citation:
Assignee:
ANGIOGENE INC (CA)
GUY LOUIS-GEORGES (CA)
GUY LOUIS-GEORGES (CA)
International Classes:
C07K14/47; C12N15/63; (IPC1-7): C07K14/47; C12N15/10; C12N15/67; A61K45/00; C12N5/06; A01K67/027
Other References:
CZYZYK-KRZESKA MARIA F ET AL: "Identification of the poly(C) binding protein in the complex associated with the 3' untranslated region of erythropoietin messenger RNA.", BLOOD, vol. 93, no. 6, 15 March 1999 (1999-03-15), pages 2111 - 2120, XP002233152, ISSN: 0006-4971
MAKEYEV A V ET AL: "A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 27 AUG 1999, vol. 274, no. 35, 27 August 1999 (1999-08-27), pages 24849 - 24857, XP002233153, ISSN: 0021-9258
KILEDJIAN M ET AL: "Purification and RNA binding properties of the polycytidylate-binding proteins alphaCP1 and alphaCP2.", METHODS (SAN DIEGO, CALIF.) UNITED STATES JAN 1999, vol. 17, no. 1, January 1999 (1999-01-01), pages 84 - 91, XP001146086, ISSN: 1046-2023
WANG ZUOREN ET AL: "The poly(A)-binding protein and an mRNA stability protein jointly regulate an endoribonuclease activity.", MOLECULAR AND CELLULAR BIOLOGY, vol. 20, no. 17, September 2000 (2000-09-01), pages 6334 - 6341, XP002233154, ISSN: 0270-7306
GUY LOUIS-GEORGES ET AL: "alphaCP1 binds to HuR: A possible role in VEGF mRNA stabilization in hypoxia.", CIRCULATION, vol. 104, no. 17 Supplement, 23 October 2001 (2001-10-23), Scientific Sessions 2001 of the American Heart Association;Anaheim, California, USA; November 11-14, 2001, October 23, 2001, pages II.202, XP009006860, ISSN: 0009-7322
MA W-J ET AL: "Cloning and Characterization of Hur, a Ubiquitously Expressed Elav-like Protein", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 271, no. 14, 5 April 1996 (1996-04-05), pages 8144 - 8151, XP002230287, ISSN: 0021-9258
YEAP BU B ET AL: "Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 26 JUL 2002, vol. 277, no. 30, 26 July 2002 (2002-07-26), pages 27183 - 27192, XP001146087, ISSN: 0021-9258
MAKEYEV A V ET AL: "A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 27 AUG 1999, vol. 274, no. 35, 27 August 1999 (1999-08-27), pages 24849 - 24857, XP002233153, ISSN: 0021-9258
KILEDJIAN M ET AL: "Purification and RNA binding properties of the polycytidylate-binding proteins alphaCP1 and alphaCP2.", METHODS (SAN DIEGO, CALIF.) UNITED STATES JAN 1999, vol. 17, no. 1, January 1999 (1999-01-01), pages 84 - 91, XP001146086, ISSN: 1046-2023
WANG ZUOREN ET AL: "The poly(A)-binding protein and an mRNA stability protein jointly regulate an endoribonuclease activity.", MOLECULAR AND CELLULAR BIOLOGY, vol. 20, no. 17, September 2000 (2000-09-01), pages 6334 - 6341, XP002233154, ISSN: 0270-7306
GUY LOUIS-GEORGES ET AL: "alphaCP1 binds to HuR: A possible role in VEGF mRNA stabilization in hypoxia.", CIRCULATION, vol. 104, no. 17 Supplement, 23 October 2001 (2001-10-23), Scientific Sessions 2001 of the American Heart Association;Anaheim, California, USA; November 11-14, 2001, October 23, 2001, pages II.202, XP009006860, ISSN: 0009-7322
MA W-J ET AL: "Cloning and Characterization of Hur, a Ubiquitously Expressed Elav-like Protein", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 271, no. 14, 5 April 1996 (1996-04-05), pages 8144 - 8151, XP002230287, ISSN: 0021-9258
YEAP BU B ET AL: "Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 26 JUL 2002, vol. 277, no. 30, 26 July 2002 (2002-07-26), pages 27183 - 27192, XP001146087, ISSN: 0021-9258
Download PDF:
Previous Patent: TUMOR ANTIGENS FOR PREVENTION AND/OR TREATMENT OF CANCER
Next Patent: USE OF LUNG CARCINOMA ANTIGEN
Next Patent: USE OF LUNG CARCINOMA ANTIGEN