Title:
UDGX-BASED TECHNOLOGY FOR DETECTING DNA DEOXYURACIL ON SINGLE-BASE RESOLUTION LEVEL
Document Type and Number:
WIPO Patent Application WO/2023/134584
Kind Code:
A1
Abstract:
The present invention provides a UdgX-based technology for detecting DNA deoxyuracil on a single-base resolution level. Specifically, the present invention provides a UdgX cross-linking and polymerase stalling sequencing ("Ucaps-seq") technology, thus achieving the detection of dU in single-base-resolution DNA. The Ucaps-seq method of the present invention is a powerful tool, and has the advantages of high sensitivity, high specificity, high resolution, etc., and has broad potential application, particularly in aspects such as disease diagnosis, evaluating gene editing accuracy, etc.
More Like This:
Inventors:
CHEN YIHAN (CN)
MA HONGHUI (CN)
MA HONGHUI (CN)
Application Number:
PCT/CN2023/071070
Publication Date:
July 20, 2023
Filing Date:
January 06, 2023
Export Citation:
Assignee:
SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE (CN)
International Classes:
C12N15/11; C12Q1/6806
Foreign References:
| CN111041023A | 2020-04-21 |
Other References:
DATTA, M. ET AL.: "Development of mCherry tagged UdgX as a highly sensitive molecular probe for specific detection of uracils in DNA", BIOCHEM BIOPHYS RES COMMUN., vol. 518, no. 1, 9 August 2019 (2019-08-09), XP085761918, DOI: 10.1016/j.bbrc.2019.08.005
STEWART JESSICA A, SCHAUER GRANT, BHAGWAT ASHOK S: "Visualization of uracils created by APOBEC3A using UdgX shows colocalization with RPA at stalled replication forks", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 48, no. 20, 18 November 2020 (2020-11-18), GB , pages e118 - e118, XP093078477, ISSN: 0305-1048, DOI: 10.1093/nar/gkaa845
TU, JIE ET AL.: "Suicide inactivation of the uracil DNA glycosylase UdgX by covalent complex formation", NAT CHEM BIOL., vol. 15, no. 6, 17 May 2019 (2019-05-17), XP036785138, DOI: 10.1038/s41589-019-0290-x
JIA, QIAN ET AL.: "Structural insights into an MsmUdgX mutant capable of both crosslinking and uracil excision capability", DNA REPAIR (AMST)., vol. 97, 13 November 2020 (2020-11-13), XP086455924, DOI: 10.1016/j.dnarep.2020.103008
STEWART JESSICA A, SCHAUER GRANT, BHAGWAT ASHOK S: "Visualization of uracils created by APOBEC3A using UdgX shows colocalization with RPA at stalled replication forks", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 48, no. 20, 18 November 2020 (2020-11-18), GB , pages e118 - e118, XP093078477, ISSN: 0305-1048, DOI: 10.1093/nar/gkaa845
TU, JIE ET AL.: "Suicide inactivation of the uracil DNA glycosylase UdgX by covalent complex formation", NAT CHEM BIOL., vol. 15, no. 6, 17 May 2019 (2019-05-17), XP036785138, DOI: 10.1038/s41589-019-0290-x
JIA, QIAN ET AL.: "Structural insights into an MsmUdgX mutant capable of both crosslinking and uracil excision capability", DNA REPAIR (AMST)., vol. 97, 13 November 2020 (2020-11-13), XP086455924, DOI: 10.1016/j.dnarep.2020.103008
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
Download PDF: