Title:
UDP-GLUCOSYLTRANSFERASE MUTANT, USE THEREOF AND METHOD FOR PREPARING REBAUDIOSIDE D
Document Type and Number:
WIPO Patent Application WO/2020/097922
Kind Code:
A1
Abstract:
Provided are a UDP-glucosyltransferase mutant, the use thereof and a method for preparing rebaudioside D. A series of mutants comprising SEQ ID NO: 3 are obtained by subjecting gene sequences of wild-type UDP-glucosyltransferase to site-directed mutagenesis, and the enzyme activity and pH stability of these mutants are improved compared with the wild-type parent.
Inventors:
FU RONGZHAO (CN)
LIU WENSHAN (CN)
LIU YUFENG (CN)
LI ZHENWEI (CN)
LIU WENSHAN (CN)
LIU YUFENG (CN)
LI ZHENWEI (CN)
Application Number:
PCT/CN2018/115937
Publication Date:
May 22, 2020
Filing Date:
November 16, 2018
Export Citation:
Assignee:
BONTAC BIO ENG SHENZHEN CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)
JIANGXI BONZYMES BIOTECHNOLOGY CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)
JIANGXI BONZYMES BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/10; C12N15/63; C12P19/00
Foreign References:
| CN107109453A | 2017-08-29 | |||
| CN101942424A | 2011-01-12 | |||
| US20150315623A1 | 2015-11-05 |
Other References:
KAZUO YAMAMOTO: "Contribution of two missense mutations (G71R and Y486D) of the bilirubin UDP glycosyltransferase (UGT1A1) gene to phenotypes of Gilbert' s syndrome and Crigler-Najjar syndrome type II", BIOCHIMICA ET BIOPHYSICA ACTA, 28 April 1998 (1998-04-28), pages 267 - 273, XP004276748, DOI: 10.1016/S0925-4439(98)00013-1
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