Title:
UDP-N- ACETYL GLUCOSAMINE: GALACTOSE- beta 1, 3-N- ACETYL GALACTOSAMINE- alpha -R/ N- ACETYL GLUCOSAMINE - beta 1, 3-N- ACETYL GALACTOSAMINE- alpha -R (GlcNAc TO GalNAc) beta 1,6-N- ACETYL GLUCOSAMINYL TRANSFERASE, C2/4GnT
Document Type and Number:
WIPO Patent Application WO2000034449
Kind Code:
A8
Abstract:
A novel gene defining a novel human UDP-GlcNAc: Gal/Gl cNAc beta 1-3GalNAc alpha beta 1, 6GlcNAc-transferase, termed C2/4GnT, with unique enzymatic properties is disclosed. The enzymatic activity of C2/4GnT is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs encoding C2/4GnT and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting C2/4GnT activity, as well as cloning and expression vectors including such DNA, cells transfected with the vectors, and recombinant methods for providing C2/4GnT. The enzyme C2/4GnT and C2/4GnT-active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of C2/4GnT. Further, the invention discloses methods of obtaining 1,6-N- acetyl glucosaminyl glycosylated saccharides, glycopeptides or glycoproteins by use of an enzymically active C2/4GnT protein or fusion protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active C2/4GnT protein as an expression system for recombinant production of such glycopeptides or glycoproteins. Also a method for the identification of DNA sequence variations in the C2/4GnT gene by isolating DNA from a patient, amplifying C2/4GnT-coding exons by PCR, and detecting the presence of DNA sequence variation, are disclosed.
More Like This:
| WO/2017/032455 | APPARATUS FOR SKINNING BODIES OF FURRED ANIMALS |
| WO/1992/010102 | CARCASS HIDE OPENING |
Inventors:
CLAUSEN HENRIK (DK)
SCHWIENTEK TILO (DK)
SCHWIENTEK TILO (DK)
Application Number:
PCT/DK1999/000677
Publication Date:
August 17, 2000
Filing Date:
December 03, 1999
Export Citation:
Assignee:
CLAUSEN HENRIK (DK)
SCHWIENTEK TILO (DK)
SCHWIENTEK TILO (DK)
International Classes:
A22B5/16; C12N15/09; A22C17/12; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12Q1/68; (IPC1-7): C12N9/10
Download PDF:
Previous Patent: HUMAN UBIQUITIN LIGASE E3 FOR THE MODULATION OF NF-KAPPA B
Next Patent: PURIFICATION OF $g(a)1-PROTEINASE INHIBITOR
Next Patent: PURIFICATION OF $g(a)1-PROTEINASE INHIBITOR