Title:
UNIVERSAL CELL FOR EXPRESSING FASLG AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2024/012420
Kind Code:
A1
Abstract:
Disclosed are a universal cell for expressing FASLG, and a preparation method therefor and the use thereof. By means of inactivating major histocompatibility complex (MHC) class I and II genes in cells and overexpressing the FASLG protein, the obtained human pluripotent stem cells or human cell lines or human induced pluripotent stem cells can further escape the killing of NK cells on the basis of escaping T cell attacks, and the effect thereof is even better than that of reported star targets CD47 and CD24. In addition, the pluripotent stem cells with a low immunogenicity retain the stemness and differentiation ability thereof.
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Inventors:
LI XIANG (CN)
ZHU MINZHE (CN)
ZHU MINZHE (CN)
Application Number:
PCT/CN2023/106663
Publication Date:
January 18, 2024
Filing Date:
July 10, 2023
Export Citation:
Assignee:
XELLSMART BIOMEDICAL SUZHOU CO LTD (CN)
International Classes:
C12N5/074; A61K35/545; A61P37/00; C07K14/47; C07K14/74; C12N5/0735; C12N5/10; C12N15/12
Domestic Patent References:
| WO2022036150A1 | 2022-02-17 | |||
| WO2018132783A1 | 2018-07-19 | |||
| WO2018227286A1 | 2018-12-20 |
Foreign References:
| CN113811362A | 2021-12-17 |
Other References:
JEFFREY HARDING, VINTERSTEN-NAGY KRISTINA, SHUTOVA MARIA, YANG HUIJUAN, TANG JEAN KIT, MASSUMI MOHAMMAD, IZAIDFAR MOHAMMAD, IZADIF: "Induction of long-term allogeneic cell acceptance and formation of immune privileged tissue in immunocompetent hosts", BIORXIV, 30 July 2019 (2019-07-30), pages 1 - 34, XP055718117, Retrieved from the Internet DOI: 10.1101/716571
MAI, XIUYING; ZHOU, MAN; LI, PING; XU, LIU: "Sequence Analysis and Structure Study of FASLG Protein", JOURNAL OF BIOLOGY, vol. 33, no. 1, 29 February 2016 (2016-02-29), pages 9 - 12, XP009552681, ISSN: 2095-1736, DOI: 10.3969/j.issn.2095-1736.2016.01.009
FLAHOU CHARLOTTE, MORISHIMA TATSUYA, TAKIZAWA HITOSHI, SUGIMOTO NAOSHI: "Fit-For-All iPSC-Derived Cell Therapies and Their Evaluation in Humanized Mice With NK Cell Immunity", FRONTIERS IN IMMUNOLOGY, FRONTIERS MEDIA, LAUSANNE, CH, vol. 12, 1 April 2021 (2021-04-01), Lausanne, CH , pages 1 - 14, XP093129574, ISSN: 1664-3224, DOI: 10.3389/fimmu.2021.662360
ZHAO, WEI ET AL.: "Strategies for Genetically Engineering Hypoimmunogenic Universal Pluripotent Stem Cells", ISCIENCE, vol. 23, 26 June 2020 (2020-06-26), XP055889918, DOI: 10.1016/j.isci.2020.101162
MAI, XIUYING; ZHOU, MAN; LI, PING; XU, LIU: "Sequence Analysis and Structure Study of FASLG Protein", JOURNAL OF BIOLOGY, vol. 33, no. 1, 29 February 2016 (2016-02-29), pages 9 - 12, XP009552681, ISSN: 2095-1736, DOI: 10.3969/j.issn.2095-1736.2016.01.009
FLAHOU CHARLOTTE, MORISHIMA TATSUYA, TAKIZAWA HITOSHI, SUGIMOTO NAOSHI: "Fit-For-All iPSC-Derived Cell Therapies and Their Evaluation in Humanized Mice With NK Cell Immunity", FRONTIERS IN IMMUNOLOGY, FRONTIERS MEDIA, LAUSANNE, CH, vol. 12, 1 April 2021 (2021-04-01), Lausanne, CH , pages 1 - 14, XP093129574, ISSN: 1664-3224, DOI: 10.3389/fimmu.2021.662360
ZHAO, WEI ET AL.: "Strategies for Genetically Engineering Hypoimmunogenic Universal Pluripotent Stem Cells", ISCIENCE, vol. 23, 26 June 2020 (2020-06-26), XP055889918, DOI: 10.1016/j.isci.2020.101162
Attorney, Agent or Firm:
BEIJING DOCVIT LAW FIRM (CN)
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