Title:
USAGE OF β-GLUCOSIDASE AND METHOD FOR PREPARING BAOHUOSIDE I BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2019/205026
Kind Code:
A1
Abstract:
Provided is a method for preparing Baohuoside I by using β-glucosidase, comprising catalyzing hydrolysis of a substrate by β-glucosidase to prepare Baohuoside I, the substrate being one or more of Epimedium extract, Icariin, Epimedin A, Epimedin B, Sagittatoside A, and Sagittatoside B, and the β-glucosidase having an amino acid sequence as shown by SEQ ID NO: 2. The method can hydrolyze Epimedium, Epimedin A, Epimedin B, Sagittatoside A, and Sagittatoside B into Baohuoside I, thereby improving the effective utilization rate of Epimedium extract, and greatly reducing the preparation cost of Baohuoside I. Also provided is application of β-glucosidase and a microbial strain containing a β-glucosidase gene in catalyzing hydrolysis of one or more of Epimedium extract, Icariin, Epimedin A, Epimedin B, Sagittatoside A, and Sagittatoside B so as to prepare Baohuoside I.
Inventors:
FU RONGZHAO (CN)
LIU LIHUI (CN)
CAO LEI (CN)
LIU TAOTAO (CN)
GUO XINGLIN (CN)
LIU LIHUI (CN)
CAO LEI (CN)
LIU TAOTAO (CN)
GUO XINGLIN (CN)
Application Number:
PCT/CN2018/084475
Publication Date:
October 31, 2019
Filing Date:
April 25, 2018
Export Citation:
Assignee:
BONTAC BIO ENG SHENZHEN CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)
International Classes:
C12P19/60; C07K14/435; C12P19/14
Foreign References:
| CN107164436A | 2017-09-15 | |||
| CN102899300A | 2013-01-30 | |||
| CN106148454A | 2016-11-23 | |||
| CN102311985A | 2012-01-11 | |||
| CN102311984A | 2012-01-11 |
Other References:
DATABASE Protein 8 January 2015 (2015-01-08), "beta-glucosidase [uncultured bacterium contig00059", XP055649368, retrieved from NCBI Database accession no. AGS52251.1
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