Title:
USE OF BCG GENE BCG_1820 IN PREPARING TUBERCULOSIS VACCINE RECOMBINANT BCG
Document Type and Number:
WIPO Patent Application WO/2023/159787
Kind Code:
A1
Abstract:
Provided are a BCG recombinant strain ΔBCG_1820 in which BCG_1820 gene is knocked out, a preparation method therefor, and the use thereof in preparing a tuberculosis vaccine. The BCG recombinant strain ΔBCG_1820 can induce macrophages to produce more antibacterial peptides, so as to endow a host with a stronger capability to resist tubercle bacillus infection, and has the potential to be a candidate vaccine for tubercle bacillus.
Inventors:
GE BAOXUE (CN)
WANG LIN (CN)
PENG CHENG (CN)
YANG HUA (CN)
WANG JIE (CN)
WANG LIN (CN)
PENG CHENG (CN)
YANG HUA (CN)
WANG JIE (CN)
Application Number:
PCT/CN2022/095396
Publication Date:
August 31, 2023
Filing Date:
May 26, 2022
Export Citation:
Assignee:
SHANGHAI PULMONARY HOSPITAL (CN)
International Classes:
C12N1/21; A61K39/04; A61P31/06; C07K14/47; C12N15/113; C12N15/31; C12N15/74; C12R1/32
Foreign References:
| US10703784B2 | 2020-07-07 | |||
| CN106148258A | 2016-11-23 | |||
| CN110283766A | 2019-09-27 | |||
| CN113322221A | 2021-08-31 | |||
| CN112481184A | 2021-03-12 |
Other References:
MEINZER ULRICH, BARREAU FREDERICK, ESMIOL-WELTERLIN SOPHIE, JUNG CAMILLE, VILLARD CLAUDE, LÉGER THIBAUT, BEN-MKADDEM SANAH, BERREB: "Yersinia pseudotuberculosis Effector YopJ Subverts the Nod2/RICK/TAK1 Pathway and Activates Caspase-1 to Induce Intestinal Barrier Dysfunction", CELL HOST & MICROBE, ELSEVIER, NL, vol. 11, no. 4, 1 April 2012 (2012-04-01), NL , pages 337 - 351, XP093087611, ISSN: 1931-3128, DOI: 10.1016/j.chom.2012.02.009
DATABASE Protein 30 June 2020 (2020-06-30), ANONYMOUS : "MULTISPECIES: type VII secretion system ESX-5 target PE18 8 [Mycobacterium tuberculosis complex]", XP093087613, retrieved from NCBI Database accession no. WP_003408976.1
EIRA CHOUDHARY, PREETI THAKUR, MADHU PAREEK, NISHEETH AGARWAL: "Gene silencing by CRISPR interference in mycobacteria", NATURE COMMUNICATIONS, vol. 6, 25 February 2015 (2015-02-25), pages 6267, XP055351970, DOI: 10.1038/ncomms7267
闫玫漪 (YAN, MEIYI): "CRISPR-Cas辅助的基因组编辑系统在分枝杆菌中的构建及应用 (Non-official translation: Construction and Application of CRISPR-Cas Assisted Genome Editing System in Mycobacteria)", 中国博士学位论文全文数据库 (基础科学辑) (BASIC SCIENCES, CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE), no. 2, 15 February 2020 (2020-02-15), ISSN: 1674-022X
WANG HAINING, JIN SHAN-SHAN, XU ZHENG-ZHONG, WANG LEI, JIAO XINRAN, CHEN XIANG: "Reviews of Gene Knockout Technology in Mycobacterium Tuberculosis", ZHONGGUO RENSHOU GONGHUANBING ZAZHI - CHINESE JOURNAL OF ZOONOSES, FUJIAN SHENG JIBING YUFANG KONGZHI ZHONGXIN, CN, vol. 36, no. 11, 29 February 2020 (2020-02-29), CN , pages 928 - 933, XP009548354, ISSN: 1002-2694
DATABASE Protein 30 June 2020 (2020-06-30), ANONYMOUS : "MULTISPECIES: type VII secretion system ESX-5 target PE18 8 [Mycobacterium tuberculosis complex]", XP093087613, retrieved from NCBI Database accession no. WP_003408976.1
EIRA CHOUDHARY, PREETI THAKUR, MADHU PAREEK, NISHEETH AGARWAL: "Gene silencing by CRISPR interference in mycobacteria", NATURE COMMUNICATIONS, vol. 6, 25 February 2015 (2015-02-25), pages 6267, XP055351970, DOI: 10.1038/ncomms7267
闫玫漪 (YAN, MEIYI): "CRISPR-Cas辅助的基因组编辑系统在分枝杆菌中的构建及应用 (Non-official translation: Construction and Application of CRISPR-Cas Assisted Genome Editing System in Mycobacteria)", 中国博士学位论文全文数据库 (基础科学辑) (BASIC SCIENCES, CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE), no. 2, 15 February 2020 (2020-02-15), ISSN: 1674-022X
WANG HAINING, JIN SHAN-SHAN, XU ZHENG-ZHONG, WANG LEI, JIAO XINRAN, CHEN XIANG: "Reviews of Gene Knockout Technology in Mycobacterium Tuberculosis", ZHONGGUO RENSHOU GONGHUANBING ZAZHI - CHINESE JOURNAL OF ZOONOSES, FUJIAN SHENG JIBING YUFANG KONGZHI ZHONGXIN, CN, vol. 36, no. 11, 29 February 2020 (2020-02-29), CN , pages 928 - 933, XP009548354, ISSN: 1002-2694
Attorney, Agent or Firm:
SHANGHAI SHENXIN LAW FIRM (CN)
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