Title:
USE OF CAS12F NUCLEASES IN PRODUCTION OF EXPRESSION MODULATED PLANT MATERIALS
Document Type and Number:
WIPO Patent Application WO/2023/150773
Kind Code:
A3
Abstract:
Methods for obtaining plant cells, plants, and plant parts, including soybean and corn plant cells, comprising Cas12F nucleases and at least one guide RNA that target the DNA regulatory sequences including a promoter region, thereby producing a gene allele with altered expression levels. The methods can be used to generate alleles of genes of interest by selecting for phenotypes conferred by the altered expression of the gene(s). Also provided are soybean plant cells and corn plant cells comprising synthetic polynucleotides that provide for gene alleles with altered expression levels.
Inventors:
KOCK MICHAEL ANDREAS (US)
DUBOIS THOMAS (US)
DUBOIS THOMAS (US)
Application Number:
PCT/US2023/062089
Publication Date:
September 28, 2023
Filing Date:
February 07, 2023
Export Citation:
Assignee:
INARI AGRICULTURE TECH INC (US)
International Classes:
C12N15/90; C12N9/22; C12N15/113; C12N15/52; C12N15/82; A01H6/46; A01H6/54
Foreign References:
| US20210395784A1 | 2021-12-23 | |||
| US20200199604A1 | 2020-06-25 | |||
| US20180327785A1 | 2018-11-15 | |||
| US20190127713A1 | 2019-05-02 | |||
| US20130055457A1 | 2013-02-28 |
Attorney, Agent or Firm:
ROMANO, Charles P. et al. (US)
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