Use of derivatives of 3-oxo-23-dihydro-5H-thiazolo[3,2-α]pyrimidine or of 3- oxo-2,3-dihydro-5H-selenazolo[3,2-αlpyrimidine or of 3-oxo-l,2,3-trihydro-5H-imidazolo[l,2-αlpyrimidine for the preparation of pharmaceutical compositions intended for the treatment of cancer

The present invention is in the field of treatment of cancer.

The present invention more precisely deals with the use of derivatives of 3-0X0-2, 3-dihydro-5H-thiazolo[3,2-α]pyrimidine or of 3-oxo-2,3-dihydro-5H-selenazolo[3,2- αjpyrimidine or of 3-oxo-l,2,3-trihydro-5H-imidazolo[l,2-α]pyrimidine for the preparation of pharmaceutical compositions intended for the treatment of cancer.

The accurate transduction of intracellular signals is achieved through processes of phosphorylation-dephosphorylation of cyclin-dependent kinase (CDK) complexes which are key regulators of essential cellular events such as cell cycle regulation, growth or differentiation. CDKs are activated by CDC25 dual-specificity phosphatases which catalyze the dephosphorylation of two adjacent phosphothreonine and phosphotyrosine residues of the CDK subunit, allowing the progression of the cell cycle into the different phases.

Three isoforms have been identified in human cells namely CDC25A, CDC25B and CDC25C. CDC25A is mainly expressed in the Gl phase and allows the progression of the cell into the S phase by dephosphorylation of the CDK2/cyclin A complex. Moreover, microinjection of CDC25A antibodies into cells induces cell arrest by blocking Gl /S transition. It has also been suggested that CDC25A is involved in mitosis. CDC25B appears to play an essential role in the G2/M transition as a mitotic starter by activating the

CDKl/Cyclin B complex whereas CDC25C regulates the entry into mitosis because of its ability to dephosphorylate CDKl/ Cyclin B complex.

The over-expression of CDC25 is frequently associated with a wide variety of tumors of high impact and is correlated with poor prognosis. For instance, CDC25 over- expression is observed in breast, colorectal, head and neck, prostate, pancreatic, or non- small-cell lung cancers. Said target is also described as being implied in gastric-cancer, hepatocarcinomas, non-ηodgkin's lymphomas, oesophagus and thyroid gland cancers.

Because CDC25 is a therapeutically relevant target, it has been screened using different experimental approaches and several classes of inhibitors have been developed in the

recent years (Lyon et al., Nat Rev Drug Discov 1 :961-976, 2002, Prevost et al., Prog Cell Cycle Res 5:225-234, 2003, Brisson et al, MoI Pharmacol 66:824-833, 2004).

A few quinone derivatives have been discovered with efficient CDC25 inhibiting activity (Brezak et al., MoI Cancer Ther 4:1378-1387, 2005) (Lazo et al., J Med Chem 44:4042-4049, 2001). However, they can be subject to undesirable oxydo-reduction reactions.

Therefore, there still exists a need to develop new chemical structures showing such CDC25 inhibiting activity.

The document WO 2004/041209 discloses a method for treating microbial infections comprising the administration of a transcription factor modulating compound of formula (IV), which can pertain to the family of 3-oxo-2,3-dihydro-5H-thiazolo[3,2- αjpyrimidine derivatives.

The document EP 1 462 105 relates to inhibitors of NF-kB and methods of treating inflammatory processes in cardio-vascular diseases by use of some 5H-thiazolo[3,2-α]pyrimidine derivatives. Moreover, document US 2004/0002526 relates to methods of treating cancer and inflammatory diseases using thiazolidinones, which modulate phospholipase D activity.

The inventors have surprisingly found that the compounds of formula (I) as defined hereinafter inhibit the enzymatic activity of CDC25. They can therefore be of particular interest in the treatment of cancer.

As a first object, the present invention deals with the use of a compound of formula (I)

wherein Ri represents a hydroxyl group, an amino group, an alkyl group, an aryl group, an alkoxy group, an alkylamino group, an aralkyl group, an arylamino group, an aryloxy group, an aralkoxy group, an aralkylamino group, a -O-(CH ₂ ) _m -CN group, where m represents 1, 2 or

3,

R ₂ represents an alkyl group, an optionally substituted phenyl group, a trifluoromethyl group,

X represents -S-, -Se- or -NR ₁₃ -, where Rn represents a hydrogen atom, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted acyl group, an aralkyl group, an aminoalkyl group, a hydroxyalkyl group, an alkoxycarbonyl group, an aralkoxycarbonyl group or a haloalkyl group,

-^ means that the exocyclic double bond may form a Z or E configuration,

R3 represents one of the following groups:

- (A)

wherein n represents 1 or 2, - (B) an optionally substituted phenyl group

wherein R ₅ , Re and R ₇ independently represent a hydrogen atom, a halogen atom, a hydroxyl group, an optionally substituted alkoxy group, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted alkenyloxy group, an acyloxy group, an optionally substituted aryloxy group, an optionally substituted phenyl group, a nitro group, an amino group, an alkylaminocarbonyl group, an acylamino group, a carboxy group, a cyano group, a malonyl group, an alkoxycarbonyl group, an acyl group, an amido group, a haloalkyl group, a hydroxyalkyl group, an aminoalkyl group, a haloalkoxy group, an aralkoxycarbonyl group, a phosphate group, a phosphonate group, a phosphonomethyl group or a phosphonodifluoromethyl group,

- (C) a pyridyl group

wherein R ₅ and Re are as defined above, - (D) a quinolinyl group or an isoquinolinyl group

wherein R ₅ and Re are as defined above, - (E) an indolyl group,

wherein R ₅ and Re groups are as defined above, - (F) a naphthyl group

wherein R ₅ and Re are as defined above, - (G) Ar

a group, where Ar may be an optionally substituted phenyl group,

(H) a thienyl group

(H) wherein R ₅ and Re are as defined above,

(J) a furyl group

(J) wherein R5 and Re are as defined above and,

- (K) a pyrrolyl group

wherein R5 and Re are as defined above, R ₄ represents one of the following groups: - (α)

wherein R ₈ , R9 and Rio independently represent a hydrogen atom, a halogen atom, a hydroxyl group, an optionally substituted alkoxy group, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted alkenyloxy group, an optionally substituted acyloxy group, an optionally substituted aryloxy group, an optionally substituted phenyl group, a nitro group, an amino group, an alkylaminocarbonyl group, an acylamino group, a carboxy group, a cyano group, a malonyl group, an alkoxycarbonyl group, an acyl group, an amido group, a haloalkyl group, a hydroxyalkyl group, an aminoalkyl group, a haloalkoxy group, an aralkoxycarbonyl group, a phosphate group, a phosphonate group, a phosphonomethyl group or a phosphonodifluoromethyl group,

- (γ) a pyridyl group

wherein R ₈ , R ₉ and Rio are as defined above,

- (δ)

wherein n represents 1 or 2,

- (ε) Ar

group, where Ar may be an optionally substituted phenyl group,

and wherein the optionally substituted group of the alkyl group, the alkoxy group, the alkenyl group and the alkenyloxy group may be one or two groups chosen among a hydroxyl group, a halogen atom, a cyano group, a carboxy group and an optionally substituted phenyl group, and wherein the optionally substituted group of the phenyl group, the aryloxy group and the alkenyl, alkyl, aryl or heteroaryl group of the acyl group may be one, two or three groups chosen among a nitro group, a halogen atom, a methyl group, a hydroxyl group, a cyano group, a carboxy group, a methoxy group or an ethoxy group, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to a particular embodiment, -^ means a Z configuration. This particular embodiment is independently valuable for all the particulars object and/or aspects developed in the following description. The present invention also provides the use of a compound of formula (I)

wherein

Ri represents a hydroxy 1 group, an amino group, an alkyl group, an aryl group, an alkoxy group, an alkylamino group, an aralkyl group, an arylamino group, an aralkoxy group, an aryloxy group, an aralkylamino group a -O-(CH2) _m -CN group, where m represents 1, 2 or 3,

R ₂ represents an alkyl group, an optionally substituted phenyl group, a trifluoromethyl group,

X represents -S- or -NRn-, wherein Rn represents a hydrogen atom, an alkyl group, an alkenyl group, an acyl group, an aralkyl group, an aminoalkyl group, a hydroxyalkyl group, an alkoxycarbonyl group, an aralkoxycarbonyl group or a haloalkyl group is as defined above,

-^ means that the exocyclic double bond may form a Z or E configuration,

R3 represents one of the following groups: - (A)

wherein n represents 1 or 2 or - (B) an optionally substituted phenyl group

wherein R ₅ , Re and R ₇ independently represent a hydrogen atom, a halogen atom, a hydroxyl group, an optionally substituted alkoxy group, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted alkenyloxy group, an acyloxy group, an optionally substituted aryloxy group, an optionally substituted phenyl group, a nitro group, an amino group, an alkylaminocarbonyl group, an acylamino group, a carboxy group, a cyano group, a malonyl group, an alkoxycarbonyl group, an acyl group, an amido group, a haloalkyl group, a hydroxyalkyl group, an aminoalkyl group, a haloalkoxy group, an aralkoxycarbonyl group,

- (C) a pyridyl group

wherein R5 and Re are as defined above,

- (D) a quinolinyl group or an isoquinolinyl group

wherein R ₅ and Re are as defined above, - (E) an indolyl group,

wherein R ₅ and Re groups are as defined above, - (F) a naphthyl group

wherein R ₅ and Re are as defined above,

- (G)

a group, where Ar may be an optionally substituted phenyl group, - (H) a thienyl group

(H)

wherein R5 and Re are as defined above, and

- (J) a furyl group

(J) wherein R5 and Re are as defined above, R ₄ represents one of the following groups:

- (α)

wherein R«, R ₉ and Rio independently represent a hydrogen atom, a halogen atom, a hydroxyl group, an optionally substituted alkoxy group, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted alkenyloxy group, an optionally substituted acyloxy group, an optionally substituted aryloxy group, an optionally substituted phenyl group, a nitro group, an amino group, an alkylaminocarbonyl group, an acylamino group, a carboxy group, a cyano group, a malonyl group, an alkoxycarbonyl group, an acyl group, an amido group, a haloalkyl group, a hydroxyalkyl group, an aminoalkyl group, a haloalkoxy group, an aralkoxycarbonyl group,

- (γ) a pyridyl group

wherein Ks, R9 and Rio are as defined above,

- (δ)

wherein n represents 1 or 2,

(ε)

group, where Ar may be an optionally substituted phenyl group, and wherein the optionally substitution group of the alkyl group, the alkoxy group, the alkenyl group and the alkenyloxy group is one or two groups chosen among a hydroxyl group, a halogen atom, a cyano group, a carboxy group and an optionally substituted phenyl group, and wherein the optionally substitution group of the phenyl group, the aryloxy group and the alkenyl, alkyl, aryl or heteroaryl group of the acyl group may be one, two or three groups chosen among a nitro group, a halogen atom, a methyl group, a hydroxyl group, a cyano group, a carboxy group, a methoxy group or an ethoxy group, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

In other words, and in connection to the preceding aspect, the present invention provides the use of a compound of formula (I) as defined above

Wherein

Ri, R ₂ , n and -^ are as defined above,

X represents -S- or -NR ₁₃ -, wherein R^ is as defined above,

R ₃ represents one group chosen among (A), (B), (C), (D), (E), (F), (G), (H) and (J) as defined above, wherein R ₅ , Re and R ₇ independently represent a hydrogen atom, a halogen atom, a hydroxyl group, an optionally substituted alkoxy group, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted alkenyloxy group, an acyloxy group, an optionally substituted aryloxy group, an optionally substituted phenyl group, a nitro group, an amino group, an alkylaminocarbonyl group, an acylamino group, a carboxy group, a cyano group, a malonyl group, an alkoxycarbonyl group, an acyl group, an amido group, a haloalkyl group, a hydroxyalkyl group, an aminoalkyl group, a haloalkoxy group, an aralkoxycarbonyl group,

R ₄ represents one group chosen among (α), (γ), (δ) and (ε) as defined above,

wherein Rs, R ₉ and Rio independently represent a hydrogen atom, a halogen atom, a hydroxyl group, an optionally substituted alkoxy group, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted alkenyloxy group, an optionally substituted acyloxy group, an optionally substituted aryloxy group, an optionally substituted phenyl group, a nitro group, an amino group, an alkylaminocarbonyl group, an acylamino group, a carboxy group, a cyano group, a malonyl group, an alkoxycarbonyl group, an acyl group, an amido group, a haloalkyl group, a hydroxyalkyl group, an aminoalkyl group, a haloalkoxy group, an aralkoxycarbonyl group, for the preparation of a pharmaceutical composition intended for the treatment of cancer, as set forth in claim 2.

The present invention moreover provides the compounds of formula (I), and (Ia) to (Ih) as defined above and hereinafter for their use for the treatment of cancer.

In the framework of the present invention, the substitution groups have the following meanings: the term "acyl" refers to an alkenyl, alkyl, aryl or heteroaryl group attached to a carbonyl group. An "acetyl" group refers to -C(O)CH ₃ , the term "acylamino" refers to an acyl group linked to an amino group, - the term "acyloxy" refers to an acyl group attached to an oxygen atom.

Included within the scope of this term are such moieties as acetoxy, n-propionoxy, isopropionoxy, n-butyroxy, valeryloxy, pivaloyloxy groups and the like, the term "alkenyl" as used herein refers to a straight or branched hydrocarbon radical containing one or two double bonds and containing from 2 to 10 carbon atoms. Included within the scope of this term are such moieties as ethenyl, propenyl, butenyl, 2-methylpropenyl and the like, the term "alkenyloxy" refers to an alkenyl group attached to an oxygen atom. Included within the scope of this term are such moieties as allyloxy, the term "alkoxy" refers to a saturated straight or branched-chain hydrocarbon radical containing from 1 to 10 carbon atoms attached to an oxygen atom. Included within the scope of this term are such moieties as methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy, t-butoxy, n-pentoxy and the like,

the term "alkoxyalkyl" refers to an alkoxy group attached to an alkyl group, the term "alkoxycarbonyl" refers to an alkoxy group attached to a carbonyl group. Included within the scope of this term are such moieties as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, butoxycarbonyl and the like, - the term "alkyl" as used herein refers to a straight or branched-chain hydrocarbon radical containing from 1 to 10 carbon atoms and cyclized manifestations thereof unless otherwise indicated. Included within the scope of this term are such moieties as methyl, ethyl, n-propyl, isopropyl cyclopropyl, n-butyl, isobutyl, s-butyl, t-butyl, t-butylmethyl, pentyl, iso-amyl, cyclopentyl, n-hexyl, cyclohexyl, cyclohexylmethyl and the like, - the term "alkylaminocarbonyl" refers to an alkylamino group attached to a carbonyl group, the term "amido" refers to an amino group attached to a carbonyl group. The term "C-amido" refers to a -C(=O)-NRR' wherein R and R' are as defined hereinafter, the term "amino" refers to -NRR' wherein R and R' are independently hydrogen atoms, alkenyl groups, alkoxyalkyl groups, alkoxycarbonyl groups, alkyl groups, acyl groups, aryl groups or aralkyl groups, the term "aminoalkyl" refers to an amino group linked to an alkyl group. Included within the scope of this term are such moieties as aminomethyl, aminoethyl and the like, - the term "aralkoxy" or "arylalkoxy" refers to an aryl group attached to an alkoxy group. Included within the scope of this term are such moieties as benzyloxy, the term "aralkoxycarbonyl" or "arylalkoxycarbonyl" refers to an aryloxy group attached to a carbonyl group. Included within the scope of this term are such moieties as benzyloxycarbonyl, - the term "aralkyl" or "arylalkyl" refers to an aryl group attached to an alkyl chain. Included within the scope of this term are such moieties as benzyl, the term "aralkylamino" refers to an aralkyl group attached to an amino group, the term "aryl" refers to an optionally substituted phenyl group, naphthyl group or biphenyl group, - the term "arylamino" refers to an aryl group attached to an amino group,

the term "aryloxy" refers to an optionally substituted aryl group attached to an oxygen atom. Included within the scope of this term are such moieties as phenoxy group, naphthyloxy group or biphenyloxy group,

- the term "carboxy" refers to -C(=O)OH, - the term "cyano" refers to -CN, the term "haloalkoxy" refers to a haloalkyl group attached to an oxygen atom, the term "haloalkyl" refers to an alkyl chain wherein one or more hydrogen atoms are replaced with a halogen atom. Included within the scope of this term are such moieties as fluoromethyl, difluoromethyl, trifluoromethyl, bromomethyl, dibromomethyl, tribromomethyl, chloromethyl, dichloromethyl, trichloromethyl and the like, the term "halogen" refers to a fluorine, a chlorine, a bromine or an iodine atom, the term "heteroaryl", alone or in combination, refers to a 5 to 7 membered unsaturated heterocyclic rings wherein at least one atom is a sulphur, a nitrogen or an oxygen atom. Included within the scope of this term are such moieties as furyl, thienyl, pyrrolyl, thiazolyl, oxazolyl, imidazolyl, pyridyl, pyrimidyl, the term "hydroxyl" refers to -OH, the term "hydroxy alkyl" refers to a hydroxyl group attached to an alkyl chain, the term "malonyl" refers to -CH(CO ₂ R) ₂ wherein R is a hydrogen atom or an alkyl group or a benzyl group, the term "phosphate" refers to -OP(O)(OR) ₂ wherein R is a hydrogen atom,an alkyl group, an aryl group or a benzyl group, the term "phosphonate" refers to -P(O)(OR) ₂ wherein R is a hydrogen atom, an alkyl group, an aryl group or a benzyl group, - the term "phosphonomethyl" refers to a phosphonate group attached to a methylene group, the term "phosphonodifluoromethyl" refers to a phosphonate group attached to a difluoromethylene group.

According to a particular embodiment, the present invention moreover provides the use of a compound of formula (I) as defined above wherein,

R3 represents one of the following groups (A) and (B):

- (A)

wherein n represents 1 or 2, and more particularly 1, - (B) an optionally substituted phenyl group

wherein R ₅ , Re and R ₇ independently represent a hydrogen atom, a halogen atom, a hydroxyl group, an optionally substituted alkoxy group, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted alkenyloxy group, an acyloxy group, an optionally substituted aryloxy group, an optionally substituted phenyl group, a nitro group, an amino group, an alkylaminocarbonyl group, an acylamino group, a carboxy group, a cyano group, a malonyl group, an alkoxycarbonyl group, an acyl group, an amido group, a haloalkyl group, a hydroxyalkyl group, an aminoalkyl group, a haloalkoxy group, an aralkoxycarbonyl group, a phosphate group, a phosphonate group, a phosphonomethyl group or a phosphonodifluoromethyl group, and wherein R ₄ represents the following group:

- (α)

wherein R«, R ₉ and Rio independently represent a hydrogen atom, a halogen atom, a hydroxyl group, an optionally substituted alkoxy group, an optionally substituted alkyl group, an optionally substituted alkenyl group, an optionally substituted alkenyloxy group, an optionally substituted acyloxy group, an optionally substituted aryloxy group, an optionally substituted phenyl group, a nitro group, an amino group, an alkylaminocarbonyl group, an acylamino group, a carboxy group, a cyano group, a malonyl group, an

alkoxycarbonyl group, an acyl group, an amido group, a haloalkyl group, a hydroxyalkyl group, an aminoalkyl group, a haloalkoxy group, an aralkoxycarbonyl group, a phosphate group, a phosphonate group, a phosphonomethyl group or a phosphonodifluoromethyl group, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to this particular embodiment, Ri is preferably a benzyloxy, an ethoxy, or a -O-(CH2)2CN, and more preferably a benzyloxy or an ethoxy, and R ₂ is a methyl group.

Still according to this particular embodiment, Rs is in the para position and represents an hydroxy group, a -CH=CH-CO ₂ H group or an acyloxy group, for example an acetoxy group, whereas at least one of R9 and Rio can be different from an hydrogen atom and

R ₉ and Rio for example independently represent an hydrogen atom, an halogen atom, in particular in the meta position or an acetoxy group (for example an acetoxy group).

According to one aspect, the present invention provides the use of a compound of formula (Ia)

wherein R ₁ , R ₂ , Rs, R9, Rio, X and n are as defined above, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a "preferred compound of formula (Ia)" wherein X represents -S- or -NH-, n represents 1,

Ri represents a hydroxyl group, a benzyloxy group, an optionally substituted (Ci-C4)alkoxy group and preferably a methoxy or an ethoxy group or an -O-(CH2) _m -CN group where m represents 1, 2 or 3 and preferably where m represents 2,

R ₂ represents a (Ci-C4)alkyl group or a phenyl group, and preferably a methyl group, and wherein Rs and R ₉ independently represent a hydrogen atom, a hydroxyl group, a

(Ci-C4)alkoxy group such as a methoxy group, an -0-CH ₂ -CO ₂ H group, a

(Ci-C4)alkenyloxy group such as an allyloxy group, a carboxy group, a -CH=CH-CO ₂ H group, a (Ci-C ₄ )alkoxycarbonyl group such as a methoxycarbonyl group, a halogen atom, a nitro group or an acyloxy group such as an acetoxy group, and Rio represents a hydrogen atom, an acyloxy group such as an acetoxy group or a halogen atom, or alternatively a methoxy group, for the preparation of a pharmaceutical composition intended for the treatment of cancer. According to one aspect, the present invention provides the use of a "preferred compound of formula (Ia)" as defined above wherein R ₂ represents a (Ci-C4)alkyl group, and preferably a methyl group, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

Among said compounds of formula (Ia), the following compounds, including their salts, may be particularly cited. The numbers given after the chemical name correspond to the numbers given in the table below:

- ethyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl-3-oxo-2,3 - dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 1

- ethyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl-3-oxo-2,3 - dihydro-5H-thiazolo[3,2-α]pyrimidinium-6-carboxylate mesylate, 2 (Ia)

- ethyl 5-(benzo[ 1 ,3]dioxol-5-yl)-2-benzylidene-7-methyl-3-oxo-2,3-dihydro-5H- thiazolo[3,2-α]pyrimidine-6-carboxylate, 3 - ethyl 2-(3,4-diallyloxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-me thyl-3-oxo-

2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 4 (2a)

- benzyl 2-(4-acetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 5 (3a)

- 2-cyanoethyl 2-(4-acetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 6 (4a) - ethyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-met hyl-3-oxo-

2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 7 (5a)

- ethyl 5-(benzo[l ,3]dioxol-5-yl)-2-(3,4-dimethoxybenzylidene)-7-methyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 8 ethyl 2-(4-acetoxy-3,5-dibromobenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 9 (6a)

- ethyl 2-(2-acetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 10

- ethyl 2-(4-acetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-methyl- 3-oxo- 1,2,3- trihydro-5H-imidazolo[l,2-α]pyrimidine-6-carboxylate, 11 (7a) - ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3,5-dibromo-4-hydroxylbenzylide ne)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 12

- (4-{[5-(benzo[l,3]dioxol-5-yl)-6-(ethoxycarbonyl)-7-methyl-3 -oxo-5H- thiazolo[3,2-α]pyrimidin-2(3H)-ylidene]methyl}phenoxy)aceti c acid, 13 (8a)

- ethyl 5-(benzo[ 1 ,3]dioxol-5-yl)-2-[4-(2-carboxyethylene)benzylidene]-7-methy l- 3-0X0-2, 3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 14 (9a)

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-met hyl-3-oxo- l,2,3-trihydro-5H-imidazolo[l,2-α]pyrimidine-6-carboxylate, 15 (10a)

- 5-(benzo[l,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl -3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylic acid, 16 (Ha) - methyl 5-(benzo[l,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl -3-oxo-

2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 17 (12a)

- ethyl 2-(4-acetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 18 ethyl 5-(benzo[l ,3]dioxol-5-yl)-7-methyl-2-(4-nitrobenzylidene)-3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 19

- ethyl 2-(3-acetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 20 (13a)

- ethyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-methoxycarbonylbenzylidene)-7-methyl- 3-0X0-2, 3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 21

- ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3-carboxybenzylidene)-7-methyl- 3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 22 (14a) - ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3-methoxycarbonylbenzylidene)-7 -methyl-

3-0X0-2, 3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 23

- 2-cyanoethyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl- 3-0X0-2, 3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 24 (15a)

- ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3,4-dihydroxylbenzylidene)-7-me thyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 25 (16a)

- ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(4-carboxybenzylidene)-7-methyl- 3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 26 (17a)

- ethyl 2-(3-acetoxy-4-hydroxylbenzylidene)-5-(benzo[ 1 ,3]dioxol-5-yl)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 27 (18a) - ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3-hydroxyl-4-methoxybenzylidene )-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 28

- benzyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-met hyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 66 (19a) ethyl 2-(3,4-diacetoxybenzylidene)-5-(2,3-dihydro-l,4-benzodioxin- 6-yl)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 67 (20a)

- benzyl 2-(3,4-diacetoxybenzylidene)- 5-(2,3-dihydro-l,4-benzodioxin-6-yl)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 68 (21a)

- ethyl 5-(benzo[l ,3]dioxol-5-yl)-2-(2-hydroxylbenzylidene)-7-methyl-3-oxo-2,3 - dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 69 - ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(4-hydroxyl-3,5-dimethoxybenzyli dene)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 70 (22a) ethyl 2-(3,4,5-triacetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 71 (23a)

- ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3,4-dichlorobenzylidene)-7-meth yl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 72 (24a)

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-3-oxo-7-phenyl- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 73 (25a)

- ethyl 2-(2,3,4-triacetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 74 (26a) ethyl 2-(2,4,5-triacetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 75 (27a) - ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3-hydroxylbenzylidene)-7-methyl -3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 76

- ethyl 5-(benzo[ 1 ,3]dioxol-5-yl)-2-(2,4-dihydroxylbenzylidene)-7-methyl-3-oxo - 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 77 (28a)

- benzyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 78 (29a)

- ethyl 5-(benzo[ 1 ,3]dioxol-5-yl)-2-(2,5-dihydroxylbenzylidene)-7-methyl-3-oxo - 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 79 (30a)

According to a particular embodiment, the present invention is directed to the use according to the present invention, wherein the used compound is chosen among the following compounds:

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-met hyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 7 (5a)

- ethyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl-3-oxo-2,3 - dihydro-5H-thiazolo[3,2-α]pyrimidinium-6-carboxylate mesylate, 2 (Ia)

- ethyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 1

- methyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 17 (12a) - ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3,5-dibromo-4-hydroxylbenzylide ne)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 12

- 2-cyanoethyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 24 (15a)

- and their salts.

According to another particular embodiment, the present invention is directed to the use according to the present invention, wherein the used compound is chosen among the following compounds:

- ethyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl-3-oxo-2,3 - dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 1 ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3,5-dibromo-4-hydroxylbenzylide ne)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 12

- ethyl 5-(benzo[ 1 ,3]dioxol-5-yl)-2-[4-(2-carboxyethylene)benzylidene]-7-methy l- 3-0X0-2, 3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 14 (9a) - methyl 5-(benzo[l,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl -3-oxo-

2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 17 (12a)

- ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3,4-dihydroxylbenzylidene)-7-me thyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 25 (16a)

- ethyl 2-(3-acetoxy-4-hydroxylbenzylidene)-5-(benzo[ 1 ,3]dioxol-5-yl)-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 27 (18a)

- benzyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-met hyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 66 (19a) ethyl 2-(2,3,4-triacetoxybenzylidene)-5-(benzo[l ,3]dioxol-5-yl)-7-methyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 74 (26a) - ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(2,4-dihydroxylbenzylidene)-7-me thyl-3-oxo-

2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 77 (28a)

- benzyl 5-(benzo[l ,3]dioxol-5-yl)-2-(4-hydroxylbenzylidene)-7-methyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 78 (29a)

- and their salts.

According to another aspect, the present invention provides the use of a compound of formula (Ib)

wherein R ₁ , R ₂ , R5, Re, R7, Rs, R9, Rio and X are as defined above, for the preparation of a pharmaceutical composition intended for the treatment of cancer. According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a "preferred compound of formula (Ib)" wherein X represents -S- or -Se-,

Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group, an arylamino group and preferably a phenylamino group or a benzyl group, R ₂ represents a (Ci-C4)alkyl group and preferably a methyl group,

R ₅ , R ₆ and R ₇ independently represent a hydrogen atom, a hydroxyl group, a

(Ci-C4)alkoxy group and preferably a methoxy group or an ethoxy group, a halogen atom, an alkoxycarbonyl group and preferably a methoxycarbonyl group, an acyloxy group such as an acetoxy group, an optionally substituted phenoxy group, a nitro group, a phenyl group, a benzyloxy group or a carboxy group, wherein the optionally substitution of the phenyl group of the phenoxy group is preferably a methoxy group or a halogen atom, and more preferably in the para position,

R8, R9, Rio independently represent a hydrogen atom, a hydroxyl group, a halogen atom, an acyloxy group, an optionally substituted (Ci-C4)alkoxy group and preferably a methoxy group or an ethoxy group, or a carboxy group, wherein the acyl group of the acyloxy group is an optionally substituted phenyl group, a furyl group, a methyl group or an ethyl group, wherein the optionally substitution of the phenyl group is a halogen atom or a (Ci-C ₄ )alkyl group, and wherein the optionally substitution of the (Ci-C4)alkoxy group is preferably a carboxy group or a halogen atom,

for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to a particular embodiment of this aspect, R ₇ is a hydrogen atom or a halogen atom, preferably in the ortho position and when R5 and R ₆ are not simultaneously hydrogen atoms, at least one of R ₅ and R ₆ is in the meta or para position.

According to another particular embodiment of this aspect, Rs is in the para position and represents a hydrogen atom, a hydroxyl group, an acyloxy group, a methoxy group, a carboxy group, or a -0-CH ₂ -CO ₂ H group, wherein the acyloxy group is an acetoxy group, a n-propionoxy group, a furylcarbonyloxy group or a phenylcarbonyloxy group optionally substituted on the phenyl group by a halogen atom or a methyl group, and R ₉ and Rio independently represent a hydrogen atom, a halogen atom, an acetoxy group, a hydroxyl group or a (Ci-C4)alkoxy group such as a methoxy group or an ethoxy group.

According to a further particular embodiment, R5, R ₆ , R ₇ , Rs, R9 and Rio, are simultaneously as defined in the two particular embodiments as defined above. According to one aspect, the present invention provides the use of a "preferred compound of formula (Ib)" as defined above wherein

X represents -S-,

Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group, an arylamino group and preferably a phenylamino group, R5, Re and R ₇ independently represent a hydrogen atom, a (Ci-C4)alkoxy group and preferably a methoxy group or an ethoxy group, a halogen atom, an acyloxy group such as an acetoxy group, an optionally substituted phenoxy group, a nitro group, a phenyl group, a hydroxy group or a carboxy group, and

Wherein the acyl group of the acyloxy group is an optionally substituted phenyl group, a furyl group or a methyl group

for the preparation of a pharmaceutical composition intended for the treatment of cancer.

Among said compounds of formula (Ib), the following compounds, including their salts, may be particularly cited. The number given after the chemical name corresponds to the numbers given in the table below: ethyl 2-(4-acetoxybenzylidene)-5-(4-acetoxyphenyl)-7-methyl-3-oxo- 2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 29 ethyl 2-(4-acetoxybenzylidene)-7-methyl-3-oxo-5-phenyl-2,3-dihydro -5H- thiazolo[3,2-α]pyrimidine-6-carboxylate, 30 ethyl 2-benzylidene-7-methyl-3-oxo-5-phenyl-2,3-dihydro-5H-thiazol o[3,2- α]pyrimidine-6-carboxylate, 31

- ethyl 5-[4-(4-chlorophenoxy)phenyl]-2-(4-hydroxylbenzylidene)-7-me thyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 32 (Ib) - ethyl 2-(4-hydroxylbenzylidene)-7-methyl-5-(4-nitrophenyl)-3-oxo-2 ,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 33 (2b) ethyl 2-(4-acetoxybenzylidene)-5-biphenyl-7-methyl-3-oxo-2,3-dihyd ro-5H- thiazolo[3,2-α]pyrimidine-6-carboxylate, 34 (3b)

- ethyl 2-(4-acetoxybenzylidene)-5 - [4-(4-methoxyphenoxy)phenyl] -7-methyl-3 - oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 35 (4b)

- ethyl 2-(4-acetoxybenzylidene)-5-(2-chlorophenyl)-7-methyl-3-oxo-2 ,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 36

- ethyl 2-(4-acetoxy-3-ethoxybenzylidene)-5-(4-chlorophenyl)-7-methy l-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 37 - ethyl 2-(3,4-dimethoxybenzylidene)-7-methyl-3-oxo-5-phenyl-2,3-dih ydro-5H- thiazo Io [3 ,2-α]pyrimidine-6-carboxylate, 38

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(3,4-dimethoxyphenyl)-7-methy l-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 39 (5b)

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(3,4-dichlorophenyl)-7-methyl -3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 40 (6b)

- ethyl 2-(4-acetoxybenzylidene)-5-(3,4-dimethoxyphenyl)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 41

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(3-carboxyphenyl)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 42 (7b)

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(4-carboxyphenyl)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 43 (8b) - N-phenyl 2-(3,4,5-trimethoxybenzylidene)-5-(3-methoxyphenyl)-7-methyl -3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxamide, 44

- ethyl 2-(2-acetoxybenzylidene)-7-methyl-3-oxo-5-phenyl-2,3-dihydro -5H- thiazolo[3,2-α]pyrimidine-6-carboxylate, 45

-(4-{[6-(ethoxycarbonyl)-5-(4-chlorophenyl)-7-methyl-3-ox o-5H-thiazolo [3,2-α]pyrimidin-2(3H)ylidene]methyl}phenoxy)acetic acid, 46 ethyl 2-(2-hydroxylbenzylidene)-5-(3-methoxyphenyl)-7-methyl-3-oxo -2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 47 ethyl 2-(5 -chloro-2-hydroxylbenzylidene)-5 -(3 -methoxyphenyl)-7-methyl-3 - oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 48 - ethyl 2-(2-ethoxybenzylidene)-5-(4-ethoxyphenyl)-7-methyl-3-oxo-2, 3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 49

- ethyl 2-(2,5-dimethoxybenzylidene)-5-(4-methoxyphenyl)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 50

- ethyl 2-(4-hydroxylbenzylidene)-7-methyl-3-oxo-5-phenyl-2,3-dihydr o-5H- thiazolo[3,2-α]pyrimidine-6-carboxylate, 51 ethyl 2-(4-carboxybenzylidene)-5-(4-methoxyphenyl)-7-methyl-3-oxo- 2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 52

- ethyl 5 -(4-methoxyphenyl)-7-methyl-2- [4- [(3 -methylbenzoyl)oxy] benzylidene]-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine -6-carboxylate, 53 - ethyl 2-[4-[(3-bromobenzoyl)oxy]-3-methoxybenzylidene)-5-(4- methoxyphenyl)-7-methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α ]pyrimidine-6-carboxylate, 54

- ethyl 2-[3-ethoxy-4-[(fur-2-yl)oxy]benzylidene]-5-(4-methoxyphenyl )-7- methyl-3-oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-car boxylate, 55

- ethyl 5-(3,4-dichlorophenyl)-2-(4-hydroxylbenzylidene)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 80 (9b)

- ethyl 2-(3,4-diacetoxybenzylidene)-7-methyl-3-oxo-5-phenyl-2,3-dih ydro-5H- thiazolo[3,2-α]pyrimidine-6-carboxylate, 81 (10b)

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(4-acetoxyphenyl)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 82 (l ib) ethyl 2-(4-hydroxylbenzylidene)-5-(4-hydroxylphenyl)-7-methyl-3-ox o-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 83 (12b) - ethyl 2-(3,4-diacetoxybenzylidene)-5-(3,4,5-trimethoxyphenyl)-7-me thyl-3-oxo-

2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 84 (13b)

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(3,4-diacetoxyphenyl)-7-methy l-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 85 (14b)

- ethyl 2-(3 ,4-diacetoxybenzylidene)-5 -(4-methoxycarbonylphenyl)-7-methyl-3 - oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 86 (15b)

- ethyl 2-(4-hydroxylbenzylidene)-5-(3,4-dihydroxylphenyl)-7-methyl- 3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 87 (16b)

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(3-acetoxyphenyl)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 88 (17b) - ethyl 2-(4-hydroxylbenzylidene)-5-(3-hydroxylphenyl)-7-methyl-3-ox o-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 89 (18b)

- ethyl 7-methyl-3-oxo-5-phenyl-2-(3,4-dipropionyloxybenzylidene)-2, 3-dihydro- 5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 90 (19b)

- benzyl 2-(3,4-diacetoxybenzylidene)-5-(2,3,4-trimethoxyphenyl)-7-me thyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 91 (20b)

- ethyl 2-(3,4-diacetoxybenzylidene)- 7-methyl-3-oxo-5-phenyl-2,3-dihydro-5H- selenazolo[3,2-α]pyrimidine-6-carboxylate, 92 (21b)

- benzyl 2-(3,4-diacetoxybenzylidene)-5-(2,4-dibenzyloxyphenyl)-7-met hyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 93 (22b) - ethyl 2-(3,4-dibenzoyloxybenzylidene)- 7-methyl-3-oxo-5-phenyl-2,3-dihydro-

5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 94 (23b)

According to a particular embodiment, the present invention is directed to the use according to the present invention, wherein the compound is ethyl 2-(3,4- diacetoxybenzylidene)-5-(3,4-dimethoxyphenyl)-7-methyl-3-oxo -2,3-dihydro-5H- thiazolo[3,2-α]pyrimidine-6-carboxylate, 39 (5b).

According to another particular embodiment, the present invention is directed to the use according to the present invention, wherein the compound is chosen among the following compounds:

- ethyl 2-(3,4-diacetoxybenzylidene)-5-(3,4-dichlorophenyl)-7-methyl -3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 40 (6b)

- ethyl 5-(3,4-dichlorophenyl)-2-(4-hydroxylbenzylidene)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 80 (9b) ethyl 2-(4-hydroxylbenzylidene)-5-(3-hydroxylphenyl)-7-methyl-3-ox o-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 89 (18b)

- and their salts.

According to another aspect, the present invention provides the use of a compound of formula (Ic)

wherein R ₁ , R ₂ , Rs, Rs>, Rio, Rn, R12, X, Y and n are as defined above, for the preparation of a pharmaceutical composition intended for the treatment of cancer chosen among the prostate cancer, hepatocarcinomas, head and neck cancer, oesophagus cancer, thyroid gland cancer and non hodgkinian lymphomas.

According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a compound of formula (Ic) wherein X represents -S-,

Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group, R ₂ represents a (Ci-C4)alkyl group and preferably a methyl group, n represents 1, Y represents -O-,

Rn and R12 represent hydrogen atoms, and

R8, R9 and Rio independently represent a hydrogen atom or a carboxy group, for the preparation of a pharmaceutical composition intended for the treatment of cancer chosen among the prostate cancer, hepatocarcinomas, head and neck cancer, oesophagus cancer, thyroid gland cancer and non hodgkinian lymphomas.

In the framework of this aspect, preferably only at most one of Rs, R9 and Rio is different of a hydrogen atom, which advantageously is on the meta position of the phenyl group.

According to another aspect, the present invention provides the use of a compound of formula (Id)

wherein R ₁ , R ₂ , R5, R ₆ , R7, Rs, R9, Rio, Rn, R12, X and Y are as defined above, for the preparation of a pharmaceutical composition intended for the treatment of cancer chosen among the prostate cancer, hepatocarcinomas, head and neck cancer, oesophagus cancer, thyroid gland cancer and non hodgkinian lymphomas.

According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a compound of formula (Id) wherein X represents -S-,

Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group, R ₂ represents a (Ci-C4)alkyl group and preferably a methyl group, Y represents -O-

Rn and Ri ₂ represent a hydrogen atom, and R8, R9 and Rio independently represent a hydrogen atom or a carboxy group, and

R5, R ₆ , R7 independently represent hydrogen atoms,

for the preparation of a pharmaceutical composition intended for the treatment of cancer chosen among the prostate cancer, hepatocarcinomas, head and neck cancer, oesophagus cancer, thyroid gland cancer and non hodgkinian lymphomas.

In the framework of this aspect, preferably only at most one of Rs, R9 and Rio is different of a hydrogen atom, which advantageously is on the meta position of the phenyl group.

According to another aspect, the present invention provides the use of a compound of formula (Ie)

wherein R ₁ , R ₂ , Rs, R9, Rio, X and n are as defined above, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a compound of formula (Ie) wherein

X represents -S-,

Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group,

R ₂ represents a (Ci-C4)alkyl group and preferably a methyl group, n represents 1 , and

Rs, R9 and Rio independently represent hydrogen atoms, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

In the framework of this aspect, preferably the nitrogen atom of the pyridyl group is on the meta position.

According to another aspect, the present invention provides the use of a compound of formula (If)

wherein R ₁ , R ₂ , R5, R ₆ , R7, X and n are as defined above, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a compound of formula (If) wherein

X represents -S-, Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group,

R ₂ represents a (Ci-C4)alkyl group and preferably a methyl group, n represents 1 , and

R ₅ , R ₆ and R ₇ independently represent hydrogen atoms, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to another aspect, the present invention provides the use of a compound of formula (Ig)

wherein R ₁ , R ₂ , R5, R ₆ , Rs, R9, Rio and X are as defined above,

for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a compound of formula (Ig) wherein X represents -S-,

Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group,

R ₂ represents a (Ci-C4)alkyl group and preferably a methyl group,

R5 and R ₆ represent hydrogen atoms or a (Ci-C4)alkyl group, preferably a methyl group and preferably on the para position , and R8, R9 and Rio represent hydrogen atoms, hydroxyl groups or acyloxy groups such as acetoxy groups, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to another aspect, the invention provides new compounds of formula (Ig), as defined above, with the proviso that at least two of Rs, R9 and Rio radical are acyloxy groups or with the further proviso that at least one of Rs, R9 and Rio radical is an acyloxy group with the exception of compounds substituted in the 2-position of the naphtyl group by a methoxy group.

According to another aspect, the present invention provides the use of a compound of formula (Ih)

wherein R ₁ , R ₂ , R5, R ₆ , Rs, R9, Rio and X are as defined above, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a compound of formula (Ih) wherein

X represents -S-,

Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group, R ₂ represents a (Ci-C4)alkyl group and preferably a methyl group,

R ₅ and R ₆ represent hydrogen atoms or a phenyl group which may optionally be substituted with a (Ci-C4)alkoxy group, and preferably a methoxy group, most preferably on the para position of the phenyl group, and

Rs, R9 and Rio independently represent hydrogen atoms or an acyloxy group and preferably an acetoxy group, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

In the framework of this aspect, preferably the nitrogen atom of the pyridyl group is on the ortho position.

Said compounds of formula (Ih) are new. Therefore, according to a further aspect, the invention provides a compound of formula (Ih) as defined above, or one of its pharmaceutically acceptable salts.

According to another aspect, the present invention provides the use of a compound of formula (Ii)

wherein

R ₁ , R ₂ , Ar, X, Rs, R9 and Rio are as defined above, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

According to a preferred embodiment of this aspect, the present invention more particularly provides the use of a compound of formula (Ii) wherein

X represents -S-,

Ri represents a (Ci-C4)alkoxy group and preferably an ethoxy group,

R ₂ represents a (Ci-C4)alkyl group and preferably a methyl group,

R8, R9 and Rio independently represent hydrogen atoms or an acyloxy group preferably an acetoxy group and Ar represents an optionally substituted phenyl group, for the preparation of a pharmaceutical composition intended for the treatment of cancer.

Some molecules represented by the general formula (I) or anyone of the formula

(Ia) to (Ii) possess one asymmetric carbon atom. They can exist in the form of pure enantiomers or as a mixture of enantiomers. Moreover, the compounds according to the invention exist in the form of free bases or of addition salts with pharmaceutically acceptable acids.

According to a particular embodiment of the invention, the cancer for which the use of the compounds of formula (I) or anyone of formula (Ia) to (Ii) is intended is chosen among the prostate cancer, the colorectal cancer, hepatocarcinomas, head and neck cancer, pancreas cancer, oesophagus cancer and non hodgkinian lymphomas, thyroid gland cancer and more particularly among the prostate cancer, the colorectal cancer, hepatocarcinomas, head and neck cancer, oesophagus cancer, thyroid gland cancer and non hodgkinian lymphomas and even more particularly among prostate cancer, hepatocarcinomas, head and neck cancer, oesophagus cancer, non hodgkinian lymphomas and thyroid gland cancer.

According to an embodiment of the invention, the use according to the present invention is intended for the preparation of a pharmaceutical composition intended for the treatment of cancer not involved with the phospho lipase D pathway.

The pharmaceutically acceptable salts of the compounds of formulae (I), (Ia) to (Ii) include the addition salts with pharmaceutically acceptable acids, such as inorganic acids, for example hydrochloric, hydrobromic, phosphoric or sulphuric acid, and organic acids, such as acetic, trifluoroacetic, propionic, oxalic, succinic, fumaric, malic, tartaric, citric, ascorbic, maleic, glutamic, benzoic, toluenesulphonic, methanesulphonic, stearic and lactic acid.

Some compounds pertaining to anyone of said hereabove defined formula (Ia) to

(Ii) form part of the present invention. It follows that the present invention provides new compounds families as set forth herein after:

- The compound of formula (Ia) wherein R ₁ , R ₂ , Rs, R9, Rio, X and n are as defined above and wherein at least one of Rs, R9 and Rio is an acyloxy group, and more particularly an acetoxy group and at least one of the said two other substituants is different from an hydrogen atom and is for example an acetoxy group. In a more particular embodiment, at least one acyloxy group is in the para position. Among these new compounds of formula (Ia) as defined above, the following may be cited: (5a), (6a), (10a), (15a), (18a), (19a), (20a), (21a), (23a), (25a), (26a) and (27a), all reported in the table hereinafter, and more particularly the compounds (18a) and (26a),

- The compound of formula (Ia) wherein R ₁ , R ₂ , Rs, R9, Rio, X and n are as defined above and wherein Rs is in the para position and represents an hydroxy group and wherein either Ri is simultaneously an aralkyl group and more particularly a benzyl group, or simultaneously one of R9 and Rio is an hydroxy group or an acyloxy group, and more particularly an acetoxy group. Among these new compounds of formula (Ia) as defined above, the following may be cited: (16a), (18a), (22a), (28a), (29a), and more particularly the compounds (18a), (16a) and (29a),

- The compound of formula (Ia) wherein R ₁ , R ₂ , Rs, R9, Rio, X and n are as defined above and wherein Rs is in the para position and represents a -CH=CHCO ₂ H group. Among these new compounds of formula (Ia) as defined above, the following may be cited: (9a), - The compound of formula (Ib) wherein R ₁ , R ₂ , R5, R ₆ , R ₇ , Rs, R9, Rio and X are as defined above and wherein at least one of Rs, R9 and Rio is an acyloxy group, and more particularly an acetoxy group and at least one of the said two other substituants is different from an hydrogen atom and is for example an acetoxy group. Among these new compounds of formula (Ib) as defined above, the following may be cited: (5b), (6b), (7b), (8b), (10b), (l ib), (13b), (14b), (15b), (17b), (19b), (20b), (21b), (22b) and (23b), and more particularly the compound (6b),

- The compound of formula (Ib) wherein R ₁ , R ₂ , R5, R ₆ , R ₇ , Rs, R9, Rio and X are as defined above and wherein Rs is in the para position and represents an hydroxy group and wherein R ₅ is in the meta position and is chosen among an hydroxy group or a halogen group, for example a chlorine atom. Among these new compounds of formula (Ib) as defined above, the following may be cited: (9b), (16b) and (18b), and more particularly the compounds (9b) and (18b), and

- The compound of formula (Ib) wherein R ₁ , R ₂ , R5, Rδ, R7, R9, Rio and X are as defined above and wherein Rs is in the para position and represents a -CH=CHCO ₂ H group.

According to an embodiment, the invention is also directed to the following new compounds:

Among compounds of formula (Ia)

(6a)

MeOCO (7a) (8a)

(13a) (14a)

(15a) (16a)

HOOC (17a) (18a)

(19a) (20a)

(22a)

(23a) (24a)

(27a) (28a)

The invention more particularly concerns the following compounds of formula

(Ia):

- compounds (2a) to (18a), and/or,

- compounds (19a) to (27a) and/or compound (30a).

Compounds (9a), (16a), (18a) and (29a) are more particularly cited as demonstrating particularly good anti-CDC25 IC50 values.

Among compounds of formula (Ib)

(2b)

(5b) (6b)

(10b)

(lib)

(23b)

The invention more particularly concerns the following compounds of formula

(Ib):

- compounds (Ib) to (8b), and/or,

- compounds (10b), (l ib), (13b), (14b), (15b), (17b) (19b) and/or (20b). Compounds (6b), (9b), (18b) are more particularly cited as demonstrating particularly good anti-CDC25 IC50 values.

Among compounds of formula (Ic)

Among compounds of formula (Ig)

MeOCO (Ig)

(2g)

Among compounds of formula (Ih)

MeOCO and to their pharmaceutically acceptable salts and to the following salifϊed compound

In accordance with a feature of the invention, the compounds of formula (I) in which X represents -S- are prepared according to scheme 1 below.

Scheme 1

(III) A compound of formula (IV) in which R3 is as defined above is reacted with a compound of formula (III) in which Ri and R ₂ are as defined above and thiourea, in the presence of an acid such as /?-toluenesulfonic acid, in a solvent such as dioxane at the boiling point of the mixture. A compound of formula (II) is obtained which is reacted with an aldehyde of formula (V) in which R ₄ is as defined above in the presence of chloroacetyl chloride, of a base such as sodium acetate in acetic anhydride and acetic acid at the boiling

point of the mixture to give a compound of formula (I) for example according to the method described by Tozkoporan (Tozkoporan et al., Arch Pharm Pharm Med Chem 331 :201-206, 1998).

When X represents S, the compounds of general formula (I) are prepared according to a further feature of the invention according to the following scheme 2.

Scheme 2

(III)

(I)

A compound of formula (IV) in which R3 is as defined above is reacted with a compound of formula (III) in which Ri and R ₂ are as defined above and thiourea, in the presence of an acid such as p-toluenesulfonic acid, in a solvent such as dioxane at the boiling point of the mixture. A compound of formula (II) is obtained which is reacted with chloroacetyl chloride in acetic anhydride and acetic acid at the boiling point of the reaction mixture to give a compound of formula (VI). A compound of formula (VI) is finally reacted with an aldehyde of formula (V) in which R ₄ is as defined above in the presence of a base such as piperidine in a solvent such as methanol to give a compound of formula (I).

When X represents NRi 3, the compounds of general formula (I) are prepared according to a further feature of the invention according to the following scheme 3.

Scheme 3

(III)

A compound of formula (IV) in which R3 is as defined above is reacted with a compound of formula (III) in which Ri and R ₂ are as defined above and guanidine hydrochloride, in the presence of a base such as NaHCOs, in a solvent such as dimethylformamide at a temperature of 70 ⁰ C for example as described by Nilsson (Nilsson et al, J Org Chem 71 :7706-7714, 2006). A compound of formula (VII) is obtained which is reacted with an aldehyde of formula (V) in which R ₄ is as defined above and chloroacetyl chloride in the presence of a base, such as sodium acetate in acetic anhydride and acetic acid at the boiling point of the reaction mixture to give a compound of formula (I) for example according to the method described by Tozkoporan (Tozkoporan et al., Arch Pharm Pharm

Med Chem 331 :201-206, 1998).

When X represents -Se-, the compounds of general formula (I) are prepared according to a further feature of the invention according to the following scheme 4.

Scheme 4

(III)

A compound of formula (IV) in which R3 is as defined above is reacted with a compound of formula (III) in which Ri and R ₂ are as defined above and selenourea, in the presence of an acid such as /?-toluenesulfonic acid, in a solvent such as dioxane at the boiling point of the mixture. A compound of formula (VIII) is obtained which is reacted with an aldehyde of formula (V) in which R ₄ is as defined above in the presence of chloroacetyl chloride, of a base such as sodium acetate in acetic anhydride and acetic acid at the boiling

point of the mixture to give a compound of formula (I) for example according to the method described by Tozkoporan (Tozkoporan et al., Arch Pharm Pharm Med Chem 331 :201-206, 1998).

The starting compounds are commercially available or are described in the literature, or can be synthesized in accordance with methods which are described therein or which are known to the person skilled in the art. In particular, compounds of formula (II), (VII) or (VIII) may be prepared following a Biginelli type reaction as previously described (Goss et al., J Comb Chem 8:153-155, 2006). For example, 4-(benzo[l,3]dioxol-5-yl)-5-ethoxycarbonyl-6-methyl-3,4- dihydropyrimidine-2(7H)-thione was prepared as following:

A solution of 5 g (33.3 mmol) of piperonal, 3.80 g (1.5 eq) of thiourea and 1.3 g (0.2 eq) of para-toluenesulfonic acid was heated for an hour under reflux in a mixture of 100 mL of dioxane and 40 ml of isopropanol. Then, 4.9 ml (1 eq) of ethyl acetoacetate were added and the mixture was refluxed for a further 18-hour. The solution was cooled to room temperature and poured into ice-water. The precipitate was filtered to give the expected compound as a white powder with a yield of 45 % (4.8 g). mp: 89-90 ⁰ C NMR ¹ H (DMSO-de, 250 MHz, δ), 1.12 (t, 3H, CH ₃ ); 2.29 (s, 3H, CH ₃ ); 4.02 (q, 2H, CH ₂ ); 5.10 (s, IH, CH); 6.00 (s, 2H, CH ₂ ); 6.67 (s, IH, aromatic H); 6.72 (d, IH, aromatic H); 6.87 (d, IH, aromatic H); 9.59 (s, IH, NH); 10.31 (s, IH, NH).

The following examples illustrate the preparation of some compounds according to the invention. The NMR spectra confirm the structures of the products obtained. The number given in brackets in the example titles correspond to those of the table which are given subsequently.

Example 1 : Compound (5a) (No. 7 of the Table)

Ethyl 2-(3,4-diacetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-met hyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate. A solution of 130 mg (0.41 mmol) of 4-(benzo[l,3]dioxol-5-yl)-5- ethoxycarbonyl-6-methyl-3,4-dihydropyrimidine-2(7H)-thione, 57 mg (0.41 mmol) of 3,4-dihydroxylbenzaldehyde, 0.03 ml (0.41 mmol) of chloroacetyl chloride, 37 mg (0.41

mmol) of sodium acetate, 1 ml of acetic anhydride and 1.5 ml of acetic acid was refluxed for 6 hours. After cooling to room temperature, the mixture was poured into ice-water and extracted with dichloromethane. The organic layer was then washed with a solution of NaHCOs, water and brine, dried over Na ₂ SO ₄ and finally evaporated under reduced pressure. The residue was purified on silica gel (eluent: cyclohexane/ ethyl acetate: 90/10) and the expected product was obtained as a yellow powder with a yield of 32% (74 mg). mp: 98°C

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.15 (t, 3H, CH ₃ ); 2.24 (s, 6H, 2X CH ₃ ); 2.40 (s, 3H, CH ₃ ); 4.07 (q, 2H, CH ₂ ); 5.99-6.01 (m, 3H, CH, CH ₂ ); 6.78-6.81 (s, 2H, aromatic H); 6.87-6.90 (d, IH, aromatic H); 7.45-7.48 (d, IH, aromatic H); 7.55-7.58 (m, 2H, aromatic H); 7.81 (s, IH, exocyclic vinylic H).

Example 2: Compound (13a) (No. 20 of the Table)

Ethyl 2-(3-acetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7-methyl- 3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate.

A solution of 200 mg (0.62 mmol) of 4-(benzo[l,3]dioxol-5-yl)-5- ethoxycarbonyl-6-methyl-3,4-dihydropyrimidine-2(7H)-thione, 76 mg (0.62 mmol) of

3-hydroxylbenzaldehyde, 0.05 ml (0.62 mmol) of chloroacetyl chloride, 51 mg (0.41 mmol) of sodium acetate, 1.5 ml of acetic anhydride and 2.3 ml of acetic acid was refluxed for 6 hours. After cooling to room temperature, the mixture was poured into ice-water and extracted with dichloromethane. The organic layer was then washed with a solution of

NaHCO ₃ , water and brine, dried over Na ₂ SO ₄ and finally evaporated under reduced pressure.

The residue was purified on silica gel (eluent: cyclohexane/ethyl acetate: 90/10) and the expected product was obtained as a yellow powder with a yield of 23% (73 mg). mp: 78°C

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.15 (t, 3H, CH ₃ ); 2.24 (s, 3H, CH ₃ ); 2.40 (s, 3H, CH ₃ ); 4.05 (q, 2H, CH ₂ ); 5.99-6.01 (m, 3H, CH, CH ₂ ); 6.78-6.81 (s, 2H, aromatic H); 6.87-6.90 (d, IH, aromatic H); 7.26-7.29 (d, IH, aromatic H); 7.40 (s, IH, aromatic H); 7.48- 7.52 (d, IH, aromatic H); 7.51-7.63 (dd, IH, aromatic H); 7.81 (s, IH, exocyclic vinylic H).

Example 3: Compound (5b) (No. 39 in the Table)

Ethyl 2-(3,4-diacetoxybenzylidene)-5-(3,4-dimethoxyphenyl)-7-methy l-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate.

A solution of 180 mg (0.55 mmol) of 5-ethoxycarbonyl-4-(3,4-dimethoxyphenyl)- 6-methyl-3,4-dihydropyrimidine-2(7H)-thione, 76 mg (0.55 mmol) of 3,4- dihydroxylbenzaldehyde, 0.05 ml (0.55 mmol) of chloroacetyl chloride, 45 mg (0.55 mmol) of sodium acetate, 1 ml of acetic anhydride and 1.5 ml of acetic acid was refluxed for 6 hours. After cooling to room temperature, the mixture was poured into ice- water and extracted with dichloromethane. The organic layer was then washed with a solution of NaηCθ3, water and brine, dried over Na ₂ SO ₄ and finally evaporated under reduced pressure. The residue was purified on silica gel (eluent: cyclohexane/ethyl acetate: 80/20) and the expected product was obtained as a yellow powder with a yield of 17% (54 mg). mp: 118°C

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.09 (t, 3H, CH ₃ ); 2.25 (s, 6H, 2X CH ₃ ); 2.33 (s, 3H, CH ₃ ); 3.66 (s, 6H, 2 X OCH ₃ ); 4.00 (q, 2H, CH ₂ ); 5.95 (s, IH, CH); 6.72-6.75 (d, IH, aromatic H); 6.81-6.88 (m, 2H, aromatic H); 7.38-7.41 (d, IH, aromatic H); 7.48-7.52 (m, 2H, aromatic H); 7.74 (s, IH, exocyclic vinylic H).

Example 4: Compound (16a) (No. 25 in the Table)

Ethyl 5-(benzo[l,3]dioxol-5-yl)-7-methyl-3-oxo-2,3-dihydro-5H-thia zolo[3,2- α]pyrimidine-6-carboxylate. A solution of 484 mg (1.51 mmol) of 4-(benzo[l,3]dioxol-5-yl)-5- ethoxycarbonyl-6-methyl-3,4-dihydropyrimidine-2(7H)-thione, 0.12 ml (1.51 mmol) of chloroacetyl chloride in 1.8 ml of acetic anhydride and 3 ml of acetic acid was refluxed for 4 hours. After cooling, the reaction mixture was poured into water. The precipitate was then filtered and washed with water to give the expected compound as a red powder with a yield of 54% (296 mg).

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.18 (t, 3H, CH ₃ ); 2.48 (s, 3H, CH ₃ ); 3.80 (dd, 2H, CH ₂ ); 4.09 (q, 2H, CH ₂ ); 5.94 (s, 2H, CH ₂ ); 5.99 (s, IH, CH); 6.69 (d, IH, aromatic H); 6.84-6.89 (m, 2H, aromatic H).

Ethyl 5-(benzo[l,3]dioxol-5-yl)-2-(3,4-dihydroxylbenzylidene)-7-me thyl-3-oxo-

2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate.

A solution of 200 mg (0.55 mmol) of ethyl 5-(benzo[l,3]dioxol-5-yl)-7-methyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 76 mg (0.55 mmol) of 3,4- dihydroxylbenzaldehyde and 2 drops of piperidine was heated under reflux in methanol (25 ml) for 2 hours. The solvent was evaporated under reduced pressure and the residue was purified on silica gel (eluent: cyclohexane/ethyl acetate: 3/2) to give the expected product as a yellow powder with a yield of 26% (31 mg). mp >260°C

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.16 (t, 3H, CH ₃ ); 2.40 (s, 3H, CH ₃ ); 4.06 (q, 2H, CH ₂ ); 5.98-6.01 (m, 3H, CH, CH ₂ ); 6.78-6.88 (m, 4H, aromatic H); 6.98-7.01 (m, 2H, aromatic H); 7.63 (m, IH, exocyclic vinylic H).

Example 5: Compound (6b) (No. 40 in the Table)

Ethyl 2-(3,4-diacetoxybenzylidene)-5-(3,4-dichlorophenyl)-7-methyl -3-oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate. A solution of 250 mg (0.72 mmol) of 4-(3,4-dichlorophenyl)-5-ethoxycarbonyl-6- methyl-3,4-dihydropyrimidine-2(7H)-thione, 100 mg (0.72 mmol) of 3,4- dihydroxylbenzaldehyde, 0.06 ml (0.72 mmol) of chloroacetyl chloride, 59 mg (0.72 mmol) of sodium acetate, 1 ml of acetic anhydride and 1.5 ml of acetic acid was refluxed for 6 hours.

After cooling to room temperature, the mixture was poured into ice-water and extracted with dichloromethane. The organic layer was then washed with a solution Of NaHCO ₃ , water and brine, dried over Na ₂ SO ₄ and finally evaporated under reduced pressure. The residue was purified on silica gel (eluent: cyclohexane/ethyl acetate: 95/5) and the expected product was obtained as a yellow powder with a yield of 34% (144 mg). mp: 95°C NMR ¹ H (CDCl ₃ , 250 MHz, δ), 1.23 (t, 3H, CH ₃ ); 2.33 (s, 6H, 2 X CH ₃ ); 2.54 (s,

3H, CH ₃ ); 4.15 (q, 2H, CH ₂ ); 6.15 (s, IH, CH); 7.25-7.42 (m, 5H, aromatic H); 7.69 (s, IH, aromatic H); 7.88 (s, IH, exocyclic vinylic H).

Example 6: Compound (9a) (N° 14 in the Table) Ethyl 5-(benzo[ 1 ,3]dioxol-5-yl)-2-[4-(2-carboxyethylene)benzylidene]-7-methy l-

3-0X0-2, 3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate

A solution of 150 mg (0.47 mmol) of 4-(benzo[l,3]dioxol-5-yl)-5-ethoxycarbonyl-6-methyl- 3,4-dihydropyrimidine-2(7H)-thione, 82 mg (0.47 mmol) of 4-formylcinnamic acid, 0.04 ml (0.48 mmol) of chloroacetyl chloride, 38 mg (0.47 mmol) of sodium acetate, 1.4 ml of acetic anhydride and 0.9 ml of acetic acid was refluxed for 6 hours. After cooling to room temperature, the mixture was poured into ice-water and extracted with dichloro methane. The organic layer was then washed with a solution of NaηCθ3, water and brine, dried over Na ₂ SO ₄ and finally evaporated under reduced pressure. The residue was purified on silica gel (eluent: dichloromethane/ethyl acetate: 98/2) and the expected product was obtained as a yellow powder with a yield of 25% (61 mg). mp >260°C

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.15 (t, 3H, CH ₃ ); 2.40 (s, 3H, CH ₃ ); 4.06 (q, 2H, CH ₂ ); 5.99 (s, IH, CH); 6.01 (s, 2H, CH ₂ ); 6.65 (d, IH, CH); 6.78-6.81 (m, 2H, aromatic H); 6.88 (d, IH, aromatic H); 7.59-7.66 (m, 3H, aromatic H, CH); 7.63 (m, 3H, exocyclic vinylic H, aromatic H).

Example 7: Compound (9b) (No. 80 in the Table)

Ethyl 5-(3,4-dichlorophenyl)-7-methyl-3-oxo-2,3-dihydro-5H-thiazol o[3,2- α]pyrimidine-6-carboxylate.

A solution of 250 mg (0.73 mmol) of 4-(3,4-dichloro)-5-ethoxycarbonyl-6- methyl-3,4-dihydropyrimidine-2(7H)-thione, 0.07 ml (0.73 mmol) of chloroacetyl chloride in

1.0 ml of acetic anhydride and 1.5 ml of acetic acid was refluxed for 4 hours. After cooling, the reaction mixture was poured into water. The precipitate was then filtered and washed with water to give the expected compound as a white powder with a yield of 86% (244 mg).

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.11 (t, 3H, CH ₃ ); 2.36 (s, 3H, CH ₃ ); 3.80 (dd, 2H, CH ₂ ); 4.04 (q, 2H, CH ₂ ); 4.27 (s, 2H, CH ₂ ); 5.87 (s, IH, CH); 7.24 (d, IH, aromatic H); 7.45 (s, IH, aromatic H); 7.64 (d, 2H, aromatic H).

Ethyl 5-(3,4-dichlorophenyl)-2-(4-hydroxylbenzylidene)-7-methyl-3- oxo-2,3- dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate. A solution of 200 mg (0.52 mmol) of ethyl 5-(3,4-dichlorophenyl)-7-methyl-3- oxo-2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate, 63 mg (0.55 mmol) of 3,4- dihydroxylbenzaldehyde and 2 drops of piperidine was heated under reflux in methanol (5 ml)

for 2 hours. The precipitate was filtered and washed with methanol to give the expected product as a yellow powder with a yield of 44% (112 mg). mp >250°C

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.14 (t, 3H, CH ₃ ); 2.42 (s, 3H, CH ₃ ); 4.07 (q, 2H, CH ₂ ); 6.05 (s, IH, CH); 6.93 (d, 2H, aromatic H); 7.31 (d, IH, aromatic H); 7.47-7.55 (m, 3H, aromatic H); 7.64 (d, IH, aromatic H); 7.73 (m, IH, exocyclic vinylic H).

Example 8: Compound (26a) (No. 74 in the Table)

Ethyl 2-(2,3,4-triacetoxybenzylidene)-5-(benzo[l,3]dioxol-5-yl)-7- methyl-3-oxo- 2,3-dihydro-5H-thiazolo[3,2-α]pyrimidine-6-carboxylate

A solution of 200 mg (0.62 mmol) of 4-(benzo[l,3]dioxol-5-yl)-5- ethoxycarbonyl-6-methyl-3,4-dihydropyrimidine-2(7H)-thione, 96 mg (0.62 mmol) of

2,3,4-trihydroxylbenzaldehyde, 0.05 ml (0.62 mmol) of chloroacetyl chloride, 51 mg (0.62 mmol) of sodium acetate, 1 ml of acetic anhydride and 1.5 ml of acetic acid was refluxed for 6 hours. After cooling to room temperature, the mixture was poured into ice-water and extracted with dichloromethane. The organic layer was then washed with a solution of

NaHCO ₃ , water and brine, dried over Na ₂ SO ₄ and finally evaporated under reduced pressure.

The residue was purified on silica gel (eluent: cyclohexane/ ethyl acetate: 3/1) and the expected product was obtained as a yellow powder with a yield of 11% (44 mg). mp: 100 ⁰ C

NMR ¹ H (DMSO-de, 250 MHz, δ), 1.15 (t, 3H, CH ₃ ); 2.32 (s, 3H, CH ₃ ); 2.35 (s, 3H, CH ₃ ); 2.39 (s, 3H, CH ₃ ); 2.41 (s, 3H, CH ₃ ); 4.06 (q, 2H, CH ₂ ); 5.97 (s, IH, CH); 6.03 (s, IH, CH ₂ ); 6.79-6.82 (s, 2H, aromatic H); 6.89 (d, IH, aromatic H); 7.46 (d, IH, aromatic H); 7.57-7.61 (m, 2H, aromatic H, exocyclic vinylic H).

The following table illustrates the chemical structures and the physical properties of some compounds according to the invention.

TABLE

(I)

Compounds of formula (If)

n (B), (δ)

No X Ri R ₂ n R ₅ R ₆ R ₇ Salt Melting point ( ⁰ C) (compound number)

61 S OEt Me 1 H H H - 128°C

In this table:

- "-" represents a compound in free form,

- OAUyI represents -0-CH ₂ -CH=CH ₂ ,

- Me represents a methyl group,

- Et represents an ethyl group,

- OMe represents a methoxy group,

- OEt represents an ethoxy group,

- OBn represents a benzyloxy group,

- Ph represents a phenyl group,

- o, p, m precises the position of the substitution on the phenyl group,

- n.d. means not determined,

Example 9: Pharmacological test

The compounds of the invention have been the subject of pharmacological tests which have demonstrated their relevance as active substances against CDC25 and on cancer cell proliferation.

MATERIAL AND METHODS

In vitro enzymatic assay

The activity of the MBP-CDC25B3 recombinant enzyme was monitored using fluorescein diphosphate as the substrate. The assay was performed in 96-well plates in a final volume of 200 μL. The maltose binding protein-CDC25B3 was kept in elution buffer [5OmM phosphate pH 7.5, 300 mM NaCl, 500 mM imidazole, 10% glycerol]. It was diluted in assay buffer [30 mM Tris-HCl (pH 8.2), 75 mM NaCl, 0.67 mM EDTA, 0.033% BSA, 1 mM DTT]. The final concentration of CDC25B3 was 75 ng/well. Products were studied in a concentration response up to 100 μM. The reaction was initiated by addition of 30 μM of fluorescein diphosphate followed by immediate measure of fluorescein emission with a Fluoroskan Ascent (Lab Systems; excitation filter: 485 nm, emission filter: 530 nm). The results are expressed as the means of at least two independent experiments with three determinations per tested concentration and per experiment. For each compound, the drug concentration required for 50% inhibition (IC50) was determined from a sigmoϊdal dose- response curve using GraphPad Prism (GraphPad Software, San Diego, CA).

Clonogenic assay

For cloning inhibition assays, HeLa cells were plated at 100 cells/well in 6-well tissue culture plates with 2.5 mL of medium/well. After 24 h growing at 37 ⁰ C in a humidified atmosphere of 5% CO ₂ , the culture medium was replaced by control medium or medium containing inhibitors at increasing concentrations (up to 100 μM) and incubated for 10 days. Cells were then washed with IM phosphate-buffered saline and fixed in 10% formaldehyde for 30 minutes at room temperature. They were carefully rinsed with water, stained with 1 mL crystal violet (2 g in 100 mL ethanol, then 2 mL in 100 mL water) for

10 minutes and finally rinsed with water. The plating efficiency was determined by counting the colonies. The results are expressed as the means of two independent experiments with three determinations per tested concentration. For each compound, the IC50 was determined from a sigmoidal dose-response using GraphPad Prism (GraphPad Software, San Diego, CA).

Cytotoxic activity

The inhibition of cell proliferation was determined using a colorimetric assay based on the cleavage of the WST-I tetrazolium salt by mitochondrial dehydrogenases in viable cells, leading to formazan formation. At day 1, LNCaP cells or MiaPaCa2 cells were plated at 5000 cells/well in 96-well culture plates with 95 μL of medium/well. At day 2, cells were treated for 96 h with 5 μL of increasing concentrations of drug. At day 6, after addition of 10 μL of WST-I per well, cells were incubated 2 hours at 37°C in a humidified atmosphere of 5% CO ₂ . Absorbance was measured at 430 nm. The results are expressed as the mean of three independent experiments with eight determinations per tested concentration and per experiment. For each compound, the IC50 value was determined from a sigmoidal dose- response using GraphPad Prism (GraphPad Software, San Diego, CA).

RESULTS The compounds of formula (I) were evaluated for in vitro enzymatic activity inhibition on the recombinant fused MBP-CDC25B protein with fluorescein diphosphate as the substrate. The tested compounds exhibit anti-CDC25 activity with IC50 values ranking from 1.2 to lOOμm, in particular from 4.5 to 100 μM, more preferably from 4.5 to 30 μM and even more preferably from 4.5 to 20 μM. IC50 values of some of the compounds of the invention are gathered in the following table A.

Therefore, the results of the tests carried out on the compounds disclosed in the present invention show that, in vitro, they exhibit the property to inhibit the enzymatic activity of CDC25B.

The effects on cell proliferation were further investigated in a clonogenic assay on HeLa cells. HeLa cells were obtained from Aptanomics (Lyon, France). HeLa cells were treated at day 1 with increasing concentration of drugs and then plated for an

additional 10-day. After crystal violet staining and colony counting, dose-response curve were drawn and the IC50 values determined. Each of the tested compounds was effective at inhibiting clonal proliferation of the HeIa cells in a concentration-dependent manner and in particular compound 1, (Ia), (5a), (5b) and 12 of the table exhibit IC50 values of 15.9, 14.8, 1.8, 3.5 and 9.75 μM respectively.

The compounds of general formula (I) were evaluated for their cytotoxic effects against LNCaP (human prostate cancer cells) and MiaPaCa2 cell line (human pancreatic cancer cells) treated with increasing concentrations of inhibitors using the WST- 1 cell viability colorimetric assay. The cell lines LNCaP and MiaPaca2 were acquired from the American Tissue Culture Collection (Rockville, Md, USA). Compounds of general formula (I) exhibit cytotoxic activity against LNCaP cell line. Cytotoxic activity against LNCaP cell line of some compounds is reported below in table A.

Compounds of general formula (I) also exhibit cytotoxic activity against

MiaPaCa2 cell line. Cytotoxic activity against MiaPaCa2 cell line of some compounds is reported below in table A.

TABLE A

n.a.: no activity at a concentration of 100 μM. n.d.: not determined.

Therefore, the results of the tests carried out on the compounds disclosed in the present invention show that, they exhibit an anticancer activity.

For this purpose an effective amount of a said compound may be administered to a subject suffering from a cancer. Among said cancer, the prostate cancer, the colorectal cancer, hepatocarcinomas, head and neck cancer, pancreas cancer, oesophagus cancer, non-hodgkinian lymphomas and thyroid gland cancer may be cited.

A further object of the invention relates to a compound of formula (Ig) with the proviso that at least two of Rs, R9 and Rio radical are acyloxy groups or with the further

proviso that at least one of Rs, R9 and Rio radical is an acyloxy group with the exception of compounds substituted in the 2-position of the naphthyl group by a methoxy group according to the invention or anyone of the individual compounds (Ia) to (30a), (Ib) to (23b), (Ic), (Ig), (2g) and (Ih), listed above, their salts with pharmaceutically acceptable acids and mixture thereof for use as a medicament.

According to another of its aspects, the present invention relates to pharmaceutical compositions containing an active ingredient, chosen among a compound of formula (Ig) with the proviso that at least two of Rs, R9 and Rio radical are acyloxy groups or with the further proviso that at least one of Rs, R9 and Rio radical is an acyloxy group with the exception of compounds substituted in the 2-position of the naphthyl group by a methoxy group or (Ih) according to the invention and anyone of the individual compounds (Ia) to (30a), (Ib) to (23b), (Ic), (Ig), (2g) and (Ih) listed above, their salts with pharmaceutically acceptable acids and mixture thereof.

Thus, these pharmaceutical compositions contain an effective amount of said compound, and one or more pharmaceutical excipients.

The aforementioned excipients are selected according to the dosage form and the desired mode of administration.

In this context the compounds according to the present invention can be present in any pharmaceutical form which is suitable for enteral or parenteral administration, in association with appropriate excipients, for example in the form of plain or coated tablets, hard gelatine and other capsules, suppositories, or drinkable or injectable solutions or suspensions, in doses which enable the daily administration of from 0.1 to 1000 mg of active substance.

The present invention according to another of its aspects, also relates to a method of treatment of cancer which comprises administering to a subject an effective amount of a compound according to formula (I), anyone of compounds of formula (Ia) to (Ii) according to the invention, anyone of the individual compounds (Ia) to (30a), (Ib) to (23b), (Ic), (Ig), (2g) and (Ih) as listed above or one of their pharmaceutically acceptable salts. The present invention according to another of its aspects, also relates to a method of treatment of cancer which comprises administering to a subject an effective amount of a compound according to formula (I), anyone of compounds of formula (Ia) to

(Ii) according to the invention, anyone of the individual compounds (Ia) to (30a), (Ib) to (23b), (Ic), (lg),(2g) and (Ih) as listed above or one of their pharmaceutically acceptable salts in combination with at least one other anti-cancer agent for a therapeutic use.