Title:
USE OF DYE TO DISTINGUISH SALT AND PROTEIN CRYSTALS UNDER MICROCRYSTALLIZATION CONDITIONS
Document Type and Number:
WIPO Patent Application WO2004011911
Kind Code:
A3
Abstract:
An improved method of screening crystal growth conditions is provided wherein molecules are crystallized from solutions containing dyes. These dyes are selectively incorporated or associated with crystals of particular character thereby rendering crystals of particular character colored and improving detection of the dyed crystals. A preferred method involves use of dyes in protein solutions overlayed by oil. Use of oil allows the use of small volumes of solution and facilitates the screening of large numbers of crystallization conditions in arrays using automated devices that dispense appropriate solutions to generate crystallization trials, overlay crystallization trials with an oil, provide appropriate conditions conducive to crystallization and enhance detection of dyed (colored) or undyed (uncolored) crystals that result.
Inventors:
COSENZA LARRY
GESTER THOMAS E
DELUCAS LAWRENCE J
BRAY TERRY L
HAMRICK DAVID T
GESTER THOMAS E
DELUCAS LAWRENCE J
BRAY TERRY L
HAMRICK DAVID T
Application Number:
PCT/US2003/024083
Publication Date:
June 10, 2004
Filing Date:
July 30, 2003
Export Citation:
Assignee:
UAB RESEARCH FOUNDATION (US)
International Classes:
C07K1/30; C30B7/00; G01N33/68; G01N25/14; (IPC1-7): G01N37/00
Foreign References:
| US6296673B1 | 2001-10-02 |
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