Title:
THE USE OF MICROFLUIDIC SYSTEMS IN THE ELECTROCHEMICAL DETECTION OF TARGET ANALYTES
Document Type and Number:
WIPO Patent Application WO2000062931
Kind Code:
A9
Abstract:
Figure (1) depicts some preferred embodiments of the invention. Figure (1A) depicts a solid support (5) that has a sample inlet port (10), a first microchannel (15), a storage module (25) (for example, for assay reagents) with a second microchannel (20). The second microchannel (20B), may be in fluid contact directly with the detection module (30) comprising a detection electrode (35), or (20A), a self-assembled monolayer and a binding ligand. Figure (1B) depicts a sample handling well (40) and a second storage well (25A) with a microchannel (20) to the sample handling well (40). For example, the sample handling well (40) could be a cell lysis chamber and the storage well (25A) could contain lysis reagents. Figure (1C) depicts a sample handling well (40) that is a cell capture or enrichment chamber, with an additional reagent storage well (25B) for elution buffer. Figure (1D) depicts the addition of a reaction module (45), with a storage module (25C), for example for storage of amplification reagents. Optional waste module (26) is connected to the reaction module (45) via a microchannel (27). All of these embodiments may addtionally comprise valves, waste wells, and pumps, including additional electrodes.
Inventors:
KAYYEM JON FAIZ (US)
Application Number:
PCT/US2000/010903
Publication Date:
November 01, 2001
Filing Date:
April 21, 2000
Export Citation:
Assignee:
CLINICAL MICRO SENSORS INC (US)
KAYYEM JON FAIZ (US)
KAYYEM JON FAIZ (US)
International Classes:
B01L3/00; G01N27/447; B81B7/02; C12M1/00; C12N15/09; C12Q1/68; C12Q1/6825; G01N27/327; G01N27/416; G01N33/543; G01N37/00; B01L7/00; B01L9/00; (IPC1-7): B01L3/00; C12Q1/68; G01N33/543
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