Title:
USE OF NITROGEN-DOPED CARBON FLUORESCENT QUANTUM DOTS IN PREPARING PRODUCTS FOR MEASURING AEROBIC GLYCOLYSIS
Document Type and Number:
WIPO Patent Application WO/2022/096029
Kind Code:
A1
Abstract:
The present invention relates to the field of glycolysis measurement, specifically to the use of nitrogen-doped carbon fluorescent quantum dots in preparing a product for measuring aerobic glycolysis, the nitrogen-doped carbon fluorescent quantum dots being one or more of N-doped graphene quantum dots, C3N4 quantum dots, C2N quantum dots, or C3N quantum dots. The product for measuring aerobic glycolysis is a reagent, and the final concentration of nitrogen-doped carbon fluorescent quantum dots in the reagent is 1 μg/mL to 1 mg/mL based on the final volume of the reagent. Fluorescent labelling of NAD+ in living cells can be performed by using the nitrogen-doped carbon fluorescent quantum dots, so that the fluorescent labelling and imaging of cells having aerobic glycolysis are realized. The method has the advantages of low costs, high efficiency, rapidness, and high accuracy, among others. At the same time, the technology contributes to the development of serial techniques such as fluorescent recognition, very early cancer warning, cancer metastasis detection, and cancer proliferation and cancer malignancy assessment, among others.
More Like This:
Inventors:
FAN XIANQUN (CN)
DING GUQIAO (CN)
ZHOU HUIFANG (CN)
YANG SIWEI (CN)
LI JIPENG (CN)
DING GUQIAO (CN)
ZHOU HUIFANG (CN)
YANG SIWEI (CN)
LI JIPENG (CN)
Application Number:
PCT/CN2021/141880
Publication Date:
May 12, 2022
Filing Date:
December 28, 2021
Export Citation:
Assignee:
SHANGHAI 9TH PEOPLES HOSPITAL SHANGHAI JIAOTONG UNIV SCHOOL MEDICINE (CN)
SHANGHAI INST MICROSYSTEM & INFORMATION TECH CAS (CN)
SHANGHAI INST MICROSYSTEM & INFORMATION TECH CAS (CN)
International Classes:
G01N21/64; B82Y20/00; C09K11/65
Foreign References:
| CN105802621A | 2016-07-27 | |||
| US20180011346A1 | 2018-01-11 | |||
| CN107287291A | 2017-10-24 | |||
| CN109661242A | 2019-04-19 |
Other References:
NIU WEN-JUN; LI YE; ZHU RONG-HUI; SHAN DAN; FAN YAN-RONG; ZHANG XUE-JI: "Ethylenediamine-assisted hydrothermal synthesis of nitrogen-doped carbon quantum dots as fluorescent probes for sensitive biosensing and bioimaging", SENSORS AND ACTUATORS B: CHEMICAL, ELSEVIER BV, NL, vol. 218, 1 January 1900 (1900-01-01), NL , pages 229 - 236, XP029238293, ISSN: 0925-4005, DOI: 10.1016/j.snb.2015.05.006
ANONYMOUS: "Team under Professor Fan Xianqun of Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine Develops New Tumor Fluorescence Imaging Technology for Early Diagnosis of Malignant Tumors", JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), vol. 41, no. 4, 30 April 2021 (2021-04-30), CN , pages 466, XP009536616, ISSN: 1674-8115
LI JIPENG, YANG SIWEI, LIU ZEYANG, WANG GANG, HE PENG, WEI WEI, YANG MUYUE, DENG YUAN, GU PING, XIE XIAOMING, KANG ZHENHUI, DING G: "Imaging Cellular Aerobic Glycolysis using Carbon Dots for Early Warning of Tumorigenesis", ADVANCED MATERIALS, VCH PUBLISHERS, DE, vol. 33, no. 1, 1 January 2021 (2021-01-01), DE , pages 2005096, XP055927524, ISSN: 0935-9648, DOI: 10.1002/adma.202005096
ANONYMOUS: "Team under Professor Fan Xianqun of Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine Develops New Tumor Fluorescence Imaging Technology for Early Diagnosis of Malignant Tumors", JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), vol. 41, no. 4, 30 April 2021 (2021-04-30), CN , pages 466, XP009536616, ISSN: 1674-8115
LI JIPENG, YANG SIWEI, LIU ZEYANG, WANG GANG, HE PENG, WEI WEI, YANG MUYUE, DENG YUAN, GU PING, XIE XIAOMING, KANG ZHENHUI, DING G: "Imaging Cellular Aerobic Glycolysis using Carbon Dots for Early Warning of Tumorigenesis", ADVANCED MATERIALS, VCH PUBLISHERS, DE, vol. 33, no. 1, 1 January 2021 (2021-01-01), DE , pages 2005096, XP055927524, ISSN: 0935-9648, DOI: 10.1002/adma.202005096
Attorney, Agent or Firm:
J.Z.M.C. PATENT AND TRADEMARK LAW OFFICE (CN)
Download PDF: