Title:
USE OF OXIDOREDUCTASE AND MUTANT THEREOF IN BIOSYNTHESIS OF NOOTKATONE
Document Type and Number:
WIPO Patent Application WO/2023/109530
Kind Code:
A1
Abstract:
Provided is the use of oxidoreductase and a mutant thereof in the biosynthesis of nootkatone. The oxidoreductase is derived from Wickerhamomyces anomalus M15 and has a high ability to convert nootkatol, and an oxidoreductase mutant having a higher ability is obtained by means of site-directed mutation. Compared with the existing oxidoreductase that is capable of catalyzing nootkatol, the oxidoreductase and the mutant thereof have a good substrate tolerance, a high conversion rate and a high salt tolerance, provide conditions for the synthesis of nootkatone using an enzyme catalytic method in vitro, and achieve the synthesis of nootkatone by means of catalyzing nootkatol in an environmentally-friendly and efficient manner.
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Inventors:
LI SHUANG (CN)
CHEN DONGYING (CN)
LI WEN (CN)
ZHU CHAOYI (CN)
CHEN DONGYING (CN)
LI WEN (CN)
ZHU CHAOYI (CN)
Application Number:
PCT/CN2022/136155
Publication Date:
June 22, 2023
Filing Date:
December 02, 2022
Export Citation:
Assignee:
UNIV SOUTH CHINA TECH (CN)
International Classes:
C12P7/26; C12N1/19; C12N9/02; C12N15/53; C12N15/81; C12R1/865
Domestic Patent References:
| WO2019059337A1 | 2019-03-28 | |||
| WO2015069847A2 | 2015-05-14 |
Foreign References:
| CN107002109A | 2017-08-01 | |||
| CN106459880A | 2017-02-22 | |||
| CN110117551A | 2019-08-13 | |||
| CN107771214A | 2018-03-06 |
Other References:
RILEY ROBERT, HARIDAS SAJEET, WOLFE KENNETH H., LOPES MARIANA R., HITTINGER CHRIS TODD, GÖKER MARKUS, SALAMOV ASAF A., WISECAVER J: "Comparative genomics of biotechnologically important yeasts", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 113, no. 35, 30 August 2016 (2016-08-30), pages 9882 - 9887, XP093071012, ISSN: 0027-8424, DOI: 10.1073/pnas.1603941113
Attorney, Agent or Firm:
HUAXUE IP AGENCY CO., LTD. (CN)
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