Title:
USE OF STEREOSELECTIVE TRANSAMINASE IN ASYMMETRIC SYNTHESIS OF CHIRAL AMINE
Document Type and Number:
WIPO Patent Application WO/2019/011236
Kind Code:
A1
Abstract:
Use of a stereoselective transaminase in the asymmetric synthesis of a chiral amine. In particular, provided is use of a polypeptide in the production of a chiral amine or a downstream product using a chiral amine as a precursor. Further provided is a method for producing a chiral amine, comprising culturing a strain expressing the polypeptide so as to obtain a chiral amine. Further provided are a chiral amine production strain and a method for constructing the chiral amine production strain. The stereoselective transaminase has a broad substrate spectrum and thus has a broad application potential in the preparation of a chiral amine.
Inventors:
CHENG ZHANBING (CN)
ZHANG TAO (CN)
TIAN ZHENHUA (CN)
QU XUDONG (CN)
DING SHAONAN (CN)
ZHANG TAO (CN)
TIAN ZHENHUA (CN)
QU XUDONG (CN)
DING SHAONAN (CN)
Application Number:
PCT/CN2018/095148
Publication Date:
January 17, 2019
Filing Date:
July 10, 2018
Export Citation:
Assignee:
ABIOCHEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/10; C12N15/54; C12P13/00
Domestic Patent References:
| WO2010099501A2 | 2010-09-02 |
Foreign References:
| US6413752B1 | 2002-07-02 | |||
| CN105018440A | 2015-11-04 | |||
| CN104480155A | 2015-04-01 | |||
| CN103014081A | 2013-04-03 | |||
| US8293507B2 | 2012-10-23 |
Other References:
DATABASE Protein [O] 9 July 2014 (2014-07-09), "aminotransferase IV [Mycolicibacterium neoaurum]", XP055564927, retrieved from NCBI Database accession no. WP_030134134.1
IWASAKI, A. ET AL.: "Microbial Synthesis of Chiral Amines by (R)-Specific Transamination with Arthrobacter Sp. KNK168", APPL. MICROBIOL. BIOTECHNOL, vol. 69, no. 5, 8 July 2005 (2005-07-08), pages 499 - 505, XP002688907
JIA, HONGHUA ET AL.: "Progress in omega -transaminase in chiral amine synthesis", MODERN CHEMICAL INDUSTRY, vol. 32, no. 3, 31 March 2012 (2012-03-31), pages 16 - 22, XP008183346
CAREYSUNDBERG: "ADVANCED ORGANIC CHEMISTRY", vol. A, 2000, PLENUM PRESS
WATSON ET AL.: "Analysis in Molecular Biology", 1987, THE BENJAMIN/CUMMINGS PUB. CO., pages: 224
CARILLO, H.LIPMAN, D., SIAM J. APPLIED MATH., vol. 48, 1988, pages 1073
"Biocomputing: Informatics and Genome Projects", 1993, ACADEMIC PRESS
"Computer Analysis of Sequence Data", 1994, HUMANA PRESS
"Sequence Analysis Primer", 1991, STOCKTON PRESS
SAMBROOK ET AL.: "Molecule Clone: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 3650537A4
IWASAKI, A. ET AL.: "Microbial Synthesis of Chiral Amines by (R)-Specific Transamination with Arthrobacter Sp. KNK168", APPL. MICROBIOL. BIOTECHNOL, vol. 69, no. 5, 8 July 2005 (2005-07-08), pages 499 - 505, XP002688907
JIA, HONGHUA ET AL.: "Progress in omega -transaminase in chiral amine synthesis", MODERN CHEMICAL INDUSTRY, vol. 32, no. 3, 31 March 2012 (2012-03-31), pages 16 - 22, XP008183346
CAREYSUNDBERG: "ADVANCED ORGANIC CHEMISTRY", vol. A, 2000, PLENUM PRESS
WATSON ET AL.: "Analysis in Molecular Biology", 1987, THE BENJAMIN/CUMMINGS PUB. CO., pages: 224
CARILLO, H.LIPMAN, D., SIAM J. APPLIED MATH., vol. 48, 1988, pages 1073
"Biocomputing: Informatics and Genome Projects", 1993, ACADEMIC PRESS
"Computer Analysis of Sequence Data", 1994, HUMANA PRESS
"Sequence Analysis Primer", 1991, STOCKTON PRESS
SAMBROOK ET AL.: "Molecule Clone: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 3650537A4
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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