Title:
USE OF ZC3H12B GENE OR PROTEIN AND ESTABLISHMENT METHOD FOR LIVER DISEASE ANIMAL MODEL
Document Type and Number:
WIPO Patent Application WO/2021/134842
Kind Code:
A1
Abstract:
Use of a Zc3h12b gene or protein thereof and an establishment method for a liver disease animal model. By using a gene editing technology, the Zc3h12b gene of medaka is targeted and knocked out, and the medaka deleting the ZC3H12B protein product is established. All exhibits different degrees of liver diseases such as hepatobiliary hyperplasia and fusion, hepatocyte fatty degeneration, and fibrosis, exhibits significant fatty liver with the increase of months of age, local cyst necrosis, obvious lymphocyte infiltration in hepatic sinusoids, and abnormal increase in the number of macrophages. Furthermore, the cells in which human tumor markers CK19, SMA and GPC3 are positive are also detected, indicating that ZC3H12B can be used as a therapeutic target and biomarker for intrahepatic cystadenoma, intrahepatic cystadenocarcinoma, or fatty liver or liver cancer related thereto. Medaka deleting Zc3h12b can be used as an animal model to study the pathological process of intrahepatic cystadenoma, intrahepatic cystadenocarcinoma or both.
Inventors:
GUAN GUIJUN (CN)
CHANG YUYANG (CN)
CHANG YUYANG (CN)
Application Number:
PCT/CN2020/072609
Publication Date:
July 08, 2021
Filing Date:
January 17, 2020
Export Citation:
Assignee:
UNIV SHANGHAI OCEAN (CN)
International Classes:
A61K45/00; A61K38/00; A61P35/00; C12N15/85
Domestic Patent References:
| WO2010126605A1 | 2010-11-04 |
Foreign References:
| CN108025048A | 2018-05-11 | |||
| CN106620721A | 2017-05-10 |
Other References:
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LIANG XIAO, ZHENG JUNHAO, GAO JIAQI: "Progress in Diagnosis snd Treatment of Intrahepatic Cystic Adenoma", CHINESE JOURNAL OF DIGESTIVE SURGERY, vol. 17, no. 12, 31 December 2018 (2018-12-31), CN, pages 1176 - 1180, XP009529230, ISSN: 1673-9752, DOI: 10.3760/cma.j.issn.1673-9752.2018.12.007
LING-FENG WAN, HONG-BING ZHAO, BO-YU XUE: "Chronic Liver Inflammation and Liver Cancer", WORLD CHINESE JOURNAL OF DIGESTOLOGY, vol. 22, no. 31, 8 November 2014 (2014-11-08), CN, pages 4757 - 4761, XP009529231, ISSN: 1009-3079, DOI: 10.11569/wcjd.v22.i31.4757
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FU MINGUI, BLACKSHEAR PERRY J.: "RNA-binding proteins in immune regulation: a focus on CCCH zinc finger proteins", NATURE REVIEWS IMMUNOLOGY, NATURE PUB. GROUP, GB, vol. 17, no. 2, 1 February 2017 (2017-02-01), GB, pages 130 - 143, XP055826443, ISSN: 1474-1733, DOI: 10.1038/nri.2016.129
QIN ZHIHUI, YANG QINGCHUAN, CHAO YUEHUI, KANG JUNMEI: "Study of CCCH-type Zinc Finger Protein", BIOTECHNOLOGY BULLETIN, no. 8, 1 January 2010 (2010-01-01), pages 1 - 6, XP055828273, DOI: 10.13560/j.cnki.biotech.bull.1985.2010.08.007
KANG YI, GUIJUN GUAN; YUNHAN HONG: "Insights of Sex Determination and Differentiation from Medaka as a Teleost Model", HEREDITAS, ZHONGGUO YICHUAN XUEHUI KEXUE, BEJING, CN, vol. 39, no. 6, 1 January 2017 (2017-01-01), CN, pages 441 - 454, XP055828269, ISSN: 0253-9772, DOI: 10.16288/j.yczz.17-140
MIAO RUIDONG, HUANG SHENGPING, ZHOU ZHOU, QUINN TIM, VAN TREECK BENJAMIN, NAYYAR TEHREEM, DIM DANIEL, JIANG ZHISHENG, PAPASIAN CHR: "Targeted disruption of MCPIP1/Zc3h12a results in fatal inflammatory disease", IMMUNOLOGY AND CELL BIOLOGY, CARLTON, AU, vol. 91, no. 5, 1 May 2013 (2013-05-01), AU, pages 368 - 376, XP055826438, ISSN: 0818-9641, DOI: 10.1038/icb.2013.11
LIANG XIAO, ZHENG JUNHAO, GAO JIAQI: "Progress in Diagnosis snd Treatment of Intrahepatic Cystic Adenoma", CHINESE JOURNAL OF DIGESTIVE SURGERY, vol. 17, no. 12, 31 December 2018 (2018-12-31), CN, pages 1176 - 1180, XP009529230, ISSN: 1673-9752, DOI: 10.3760/cma.j.issn.1673-9752.2018.12.007
LING-FENG WAN, HONG-BING ZHAO, BO-YU XUE: "Chronic Liver Inflammation and Liver Cancer", WORLD CHINESE JOURNAL OF DIGESTOLOGY, vol. 22, no. 31, 8 November 2014 (2014-11-08), CN, pages 4757 - 4761, XP009529231, ISSN: 1009-3079, DOI: 10.11569/wcjd.v22.i31.4757
ZHOU ZEWEI, JIANG RONG, YANG XIYUE, GUO HUIFANG, FANG SHENCUN, ZHANG YINGMING, CHENG YUSI, WANG JIANG, YAO HONGHONG, CHAO JIE: "circRNA Mediates Silica-Induced Macrophage Activation Via HECTD1/ZC3H12A-Dependent Ubiquitination", THERANOSTICS, IVYSPRING INTERNATIONAL PUBLISHER, AU, vol. 8, no. 2, 1 January 2018 (2018-01-01), AU, pages 575 - 592, XP055826441, ISSN: 1838-7640, DOI: 10.7150/thno.21648
FU MINGUI, BLACKSHEAR PERRY J.: "RNA-binding proteins in immune regulation: a focus on CCCH zinc finger proteins", NATURE REVIEWS IMMUNOLOGY, NATURE PUB. GROUP, GB, vol. 17, no. 2, 1 February 2017 (2017-02-01), GB, pages 130 - 143, XP055826443, ISSN: 1474-1733, DOI: 10.1038/nri.2016.129
QIN ZHIHUI, YANG QINGCHUAN, CHAO YUEHUI, KANG JUNMEI: "Study of CCCH-type Zinc Finger Protein", BIOTECHNOLOGY BULLETIN, no. 8, 1 January 2010 (2010-01-01), pages 1 - 6, XP055828273, DOI: 10.13560/j.cnki.biotech.bull.1985.2010.08.007
KANG YI, GUIJUN GUAN; YUNHAN HONG: "Insights of Sex Determination and Differentiation from Medaka as a Teleost Model", HEREDITAS, ZHONGGUO YICHUAN XUEHUI KEXUE, BEJING, CN, vol. 39, no. 6, 1 January 2017 (2017-01-01), CN, pages 441 - 454, XP055828269, ISSN: 0253-9772, DOI: 10.16288/j.yczz.17-140
Attorney, Agent or Firm:
SUNHOLD LAW FIRM (CN)
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