Title:
USES FOR LONG POLAR FIMBRIAE GENES OF PATHOGENIC ESCHERICHIA COLI STRAINS
Document Type and Number:
WIPO Patent Application WO/2010/117465
Kind Code:
A3
Abstract:
Provided are methods for identifying lpf genes in pathogenic serotypes of the Enterobacteriaceae family and for differentiating Escherichia coli (E. coli) O157:H7 strains in an isolate using primer pairs specific to lpf gene variants, particularly IpfA1 and/ or lpfA 2 genes and amplicon size of the PCR product to identify prototypic Enterobacteriaceae serotypes or more specifically, to differentiate strains of E. coli serotype O157:1-17. Differentiation further requires identifying the E. coli isolate's eae gene variant type which in combination with the IpfA1 and/or lpfA2 variant identification provides unique markers. Also provided are the primer pairs and a kit comprising the same.
Inventors:
TORRES ALFREDO G (US)
Application Number:
PCT/US2010/001065
Publication Date:
June 16, 2011
Filing Date:
April 09, 2010
Export Citation:
Assignee:
UNIV TEXAS (US)
TORRES ALFREDO G (US)
TORRES ALFREDO G (US)
International Classes:
C12Q1/68; C12N15/31; C12R1/19
Other References:
TORRES ET AL.: "Identification and Characterization of lpfABCC'DE, a Fimbrial Operon of Enterohemorrhagic Escherichia coli O157:H7", INFECTION AND IMMUNITY, vol. 70, no. 10, pages 5416 - 5427
TORRES ET AL.: "Environmental regulation and colonization attributes of the long polar fimbriae (LPF) of Escherichia coli O157:H7", INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, vol. 297, no. 3, 11 June 2007 (2007-06-11), pages 177 - 185, XP022063057
LEDEBOER ET AL.: "Salmonella enterica Serovar Typhimurium Requires the Lpf, Pef, and Tafi Fimbriae for Biofilm Formation on HEp-2 Tissue Culture Cells and Chicken Intestinal Epithelium", INFECTION AND IMMUNITY, vol. 74, no. 6, June 2006 (2006-06-01), pages 3156 - 3169
LOW ET AL.: "Analysis of fimbrial gene clusters and their expression in enterohaemorrhagic Escherichia coli O157:H7", ENVIRON. MICROBIOL., vol. 8, no. 6, June 2006 (2006-06-01), pages 1033 - 1047
TORRES ET AL.: "Environmental regulation and colonization attributes of the long polar fimbriae (LPF) of Escherichia coli O157:H7", INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, vol. 297, no. 3, 11 June 2007 (2007-06-11), pages 177 - 185, XP022063057
LEDEBOER ET AL.: "Salmonella enterica Serovar Typhimurium Requires the Lpf, Pef, and Tafi Fimbriae for Biofilm Formation on HEp-2 Tissue Culture Cells and Chicken Intestinal Epithelium", INFECTION AND IMMUNITY, vol. 74, no. 6, June 2006 (2006-06-01), pages 3156 - 3169
LOW ET AL.: "Analysis of fimbrial gene clusters and their expression in enterohaemorrhagic Escherichia coli O157:H7", ENVIRON. MICROBIOL., vol. 8, no. 6, June 2006 (2006-06-01), pages 1033 - 1047
Attorney, Agent or Firm:
ADLER, Benjamin, Aaron (8011 Candle Ln.Houston, TX, US)
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