Title:
USES OF A THYX POLYPEPTIDE OR A NUCLEIC ACID ENCODING SUCH A POLYPEPTIDE, IN PARTICULAR FOR SCREENING ANTI-BACTERIAL OR ANTI-VIRAL COMPOUNDS
Document Type and Number:
WIPO Patent Application WO2003012108
Kind Code:
A3
Abstract:
The invention concerns various uses of a novel family of enzymes, called THYX, capable of catalyzing the synthesis of thymidine 5'-monophosphate in the absence of an active thymidylate synthase enzyme (ThyA). The invention also concerns reaction media for the thymidylate synthase activity of a THYX polypeptide and screening methods using said reaction media, as well as kits for implementing said methods.
Inventors:
MYLLYKALLIO HANNU (FR)
LIEBL URSULA (FR)
LEDUC DAMIEN (FR)
LIPOWSKI GERARD (FR)
MARTIN JEAN-LOUIS (FR)
LIEBL URSULA (FR)
LEDUC DAMIEN (FR)
LIPOWSKI GERARD (FR)
MARTIN JEAN-LOUIS (FR)
Application Number:
PCT/FR2002/002795
Publication Date:
March 04, 2004
Filing Date:
August 02, 2002
Export Citation:
Assignee:
INST NAT SANTE RECH MED (FR)
CENTRE NAT RECH SCIENT (FR)
UNIV PARIS SUD (FR)
MYLLYKALLIO HANNU (FR)
LIEBL URSULA (FR)
LEDUC DAMIEN (FR)
LIPOWSKI GERARD (FR)
MARTIN JEAN-LOUIS (FR)
CENTRE NAT RECH SCIENT (FR)
UNIV PARIS SUD (FR)
MYLLYKALLIO HANNU (FR)
LIEBL URSULA (FR)
LEDUC DAMIEN (FR)
LIPOWSKI GERARD (FR)
MARTIN JEAN-LOUIS (FR)
International Classes:
C12N15/54; C12Q1/18; C12Q1/48; G01N33/573; (IPC1-7): C12N15/54; C12N9/10; C12N15/11; C12P19/38; C12Q1/48; C12Q1/68
Other References:
GALPERIN MICHAEL Y ET AL: "Who's your neighbor? New computational approaches for functional genomics", NATURE BIOTECHNOLOGY, vol. 18, no. 6, June 2000 (2000-06-01), pages 609 - 613, XP002262588, ISSN: 1087-0156
DYNES J L ET AL: "MOLECULAR COMPLEMENTATION OF A GENETIC MARKER IN DICTYOSTELIUM USING A GENOMIC DNA LIBRARY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 86, no. 20, 1989, 1989, pages 7966 - 7970, XP002200055, ISSN: 0027-8424
DATABASE EMBL [online] 19 December 1997 (1997-12-19), XP002200058, Database accession no. AE000790
DATABASE EMBL [online] 17 July 1998 (1998-07-17), XP002200059, Database accession no. AE001268
DATABASE EMBL [online] 30 August 2000 (2000-08-30), XP002200060, Database accession no. AF189021
DATABASE EMBL [online] 4 June 1999 (1999-06-04), XP002200061, Database accession no. AE001723
MYLLYKALLIO HANNU ET AL: "An alternative flavin-dependent mechanism for thymidylate synthesis", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 297, no. 5578, 23 May 2002 (2002-05-23), pages 105 - 107, XP002235740, ISSN: 0036-8075
IYER LM ET AL: "Quod erat demonstrandum? The mystery of experimental validation of apparently erroneous computational analyses of protein sequences", GENOME BIOLOGY, vol. 2, no. 12, 13 November 2001 (2001-11-13), pages research0051.1 - research0051.11, XP002200056, ISSN: 1465-6914, Retrieved from the Internet [retrieved on 20020523]
TATUSOV ROMAN L ET AL: "The COG database: New developments in phylogenetic classification of proteins from complete genomes.", NUCLEIC ACIDS RESEARCH, vol. 29, no. 1, 1 January 2001 (2001-01-01), pages 22 - 28, XP002200057, ISSN: 0305-1048
DELK A S ET AL: "METHYLENE TETRA HYDRO FOLATE DEPENDENT BIOSYNTHESIS OF RIBO THYMIDINE IN TRANSFER RNA OF STREPTOCOCCUS-FAECALIS EVIDENCE FOR REDUCTION OF THE 1 CARBON UNIT BY FADH-2", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 255, no. 10, 1980, pages 4387 - 4390, XP002235752, ISSN: 0021-9258
CARRERAS C W ET SANTI D V: "The catalytic mechanism and structure of thymidylate synthase", ANNUAL REVIEW OF BIOCHEMISTRY, PALTO ALTO, CA, US, vol. 64, 1995, pages 721 - 762, XP002093507, ISSN: 0066-4154
DYNES J L ET AL: "MOLECULAR COMPLEMENTATION OF A GENETIC MARKER IN DICTYOSTELIUM USING A GENOMIC DNA LIBRARY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 86, no. 20, 1989, 1989, pages 7966 - 7970, XP002200055, ISSN: 0027-8424
DATABASE EMBL [online] 19 December 1997 (1997-12-19), XP002200058, Database accession no. AE000790
DATABASE EMBL [online] 17 July 1998 (1998-07-17), XP002200059, Database accession no. AE001268
DATABASE EMBL [online] 30 August 2000 (2000-08-30), XP002200060, Database accession no. AF189021
DATABASE EMBL [online] 4 June 1999 (1999-06-04), XP002200061, Database accession no. AE001723
MYLLYKALLIO HANNU ET AL: "An alternative flavin-dependent mechanism for thymidylate synthesis", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 297, no. 5578, 23 May 2002 (2002-05-23), pages 105 - 107, XP002235740, ISSN: 0036-8075
IYER LM ET AL: "Quod erat demonstrandum? The mystery of experimental validation of apparently erroneous computational analyses of protein sequences", GENOME BIOLOGY, vol. 2, no. 12, 13 November 2001 (2001-11-13), pages research0051.1 - research0051.11, XP002200056, ISSN: 1465-6914, Retrieved from the Internet
TATUSOV ROMAN L ET AL: "The COG database: New developments in phylogenetic classification of proteins from complete genomes.", NUCLEIC ACIDS RESEARCH, vol. 29, no. 1, 1 January 2001 (2001-01-01), pages 22 - 28, XP002200057, ISSN: 0305-1048
DELK A S ET AL: "METHYLENE TETRA HYDRO FOLATE DEPENDENT BIOSYNTHESIS OF RIBO THYMIDINE IN TRANSFER RNA OF STREPTOCOCCUS-FAECALIS EVIDENCE FOR REDUCTION OF THE 1 CARBON UNIT BY FADH-2", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 255, no. 10, 1980, pages 4387 - 4390, XP002235752, ISSN: 0021-9258
CARRERAS C W ET SANTI D V: "The catalytic mechanism and structure of thymidylate synthase", ANNUAL REVIEW OF BIOCHEMISTRY, PALTO ALTO, CA, US, vol. 64, 1995, pages 721 - 762, XP002093507, ISSN: 0066-4154
Download PDF: