Title:
VACCINE PLATFORM FOR PRODUCING FOOT AND MOUTH DISEASE VIRUS-LIKE PARTICLES
Document Type and Number:
WIPO Patent Application WO/2023/101381
Kind Code:
A1
Abstract:
The present invention relates to a polynucleotide in which the 3C, 3D cleavage site, VP4, VP2, VP3, and VP1 base sequences of foot and mouse disease virus (FMDV) are sequentially connected, a recombinant vector carrying same, a transformant transformed with the recombinant vector, a method for producing foot and mouth disease virus-like particles using the transformant, and a vaccine composition using a food and mouth disease virus-like particle protein that assembles by itself using the polynucleotide. When a vaccine is prepared using the FMDV VLP of the present invention as an antigen, the period of antibody production in mice is short, the survival rate against foot and mouth disease virus challenge is high, and the neutralizing antibody titer is high. In addition, it was observed that antibody production started within about 2 weeks in pigs as well as mice and the PI value was more than 50% even after 6 weeks, so that the vaccine can be applied as a vaccine platform for producing foot and mouth disease virus-like particles.
Inventors:
KIM BONG YOON (KR)
Application Number:
PCT/KR2022/019148
Publication Date:
June 08, 2023
Filing Date:
November 30, 2022
Export Citation:
Assignee:
WATSON RND INC (KR)
International Classes:
C07K14/005; A23K20/153; A61K39/00; A61K39/135; A61P31/14
Foreign References:
| KR20190030223A | 2019-03-21 | |||
| KR20170058430A | 2017-05-26 | |||
| KR20120114217A | 2012-10-16 |
Other References:
BELSHAM GRAHAM J.: "Towards improvements in foot-and-mouth disease vaccine performance", ACTA VETERINARIA SCANDINAVICA, vol. 62, no. 1, 1 December 2020 (2020-12-01), pages 20, XP093068682, DOI: 10.1186/s13028-020-00519-1
HYE-YOUNG JEOUNG; WON-HA LEE; WOOSEOG JEONG; YOUNG-JOON KO; CHEONG-UP CHOI; DONG-JUN AN;: "Immune responses and expression of the virus-like particle antigen of the porcine encephalomyocarditis virus", RESEARCH IN VETERINARY SCIENCE, BRITISH VETERINARY ASSOCIATION, LONDON., GB, vol. 89, no. 2, 8 March 2010 (2010-03-08), GB , pages 295 - 300, XP028302130, ISSN: 0034-5288, DOI: 10.1016/j.rvsc.2010.03.012
HYE-YOUNG JEOUNG; WON-HA LEE; WOOSEOG JEONG; YOUNG-JOON KO; CHEONG-UP CHOI; DONG-JUN AN;: "Immune responses and expression of the virus-like particle antigen of the porcine encephalomyocarditis virus", RESEARCH IN VETERINARY SCIENCE, BRITISH VETERINARY ASSOCIATION, LONDON., GB, vol. 89, no. 2, 8 March 2010 (2010-03-08), GB , pages 295 - 300, XP028302130, ISSN: 0034-5288, DOI: 10.1016/j.rvsc.2010.03.012
Attorney, Agent or Firm:
ECM IP & LAW FIRM (KR)
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