Title:
VACCINE USED FOR PREVENTING TOXOPLASMA GONDII INFECTION AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2019/242177
Kind Code:
A1
Abstract:
Provided by the present invention is a protein having Toxoplasma immunogenicity, the protein being a cyclophilin mutant protein and consisting of the amino acid sequence as shown in SEQ ID 2. Further provided by the present invention is a nucleic acid that may encode a protein having Toxoplasma immunogenicity, which has the nucleic acid sequence as shown in SEQ ID 1. Further provided by the present invention is a vaccine, which is obtained by double-digesting a Toxoplasma antigen gene and then linking same to a prokaryotic expression vector such as pET28a, and transforming same into a prokaryotic expression engineering strain such as BL21(DE3), thereby inducing the high-efficiency expression thereof, wherein the purified protein is a soluble protein which maintains the specific immunogenicity thereof.
Inventors:
HOU FENG (CN)
CAO LILI (CN)
GONG PENGTAO (CN)
LI SIMING (CN)
CHEN XINGYUAN (CN)
DING HE (CN)
WANG DIAN (CN)
CAO LILI (CN)
GONG PENGTAO (CN)
LI SIMING (CN)
CHEN XINGYUAN (CN)
DING HE (CN)
WANG DIAN (CN)
Application Number:
PCT/CN2018/111235
Publication Date:
December 26, 2019
Filing Date:
October 22, 2018
Export Citation:
Assignee:
HOU FENG (CN)
International Classes:
C07K14/45; A61K39/002; A61P33/02; C12N1/21; C12N15/30; C12N15/70
Other References:
HIGH KP ET AL.: "Isolation, cDNA Sequences, and Biochemical Characterization of the Major Cyclosporin-Binding Proteins of Toxoplasma Gondii", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 12, 25 March 1994 (1994-03-25), pages 9105 - 9112, XP055667727
LI, YUN'NA ET AL.: "Cloning and Prokaryotic Expression of Cyclophilin Gene of Toxoplasma Gondii", CHINESE JOURNAL OF BIOLOGICALS, vol. 23, no. 9, 30 September 2010 (2010-09-30), pages 961 - 966, XP055770951
HUANG, JIN'GUI ET AL.: "Studies on Immuno-Protection from a Toxoplasma Gondii Infection Provided by a TgCyP Subunit Vaccine", JOURNAL OF PATHOGEN BIOLOGY, vol. 6, no. 7, 31 July 2011 (2011-07-31), pages 517 - 520, XP055770979
LI YUNNA, CLONING AND PROKARYOTIC EXPRESSION OF TOXOPLASMA GONDII CYCLOPHILIN GENE, 2010
See also references of EP 3838916A4
LI, YUN'NA ET AL.: "Cloning and Prokaryotic Expression of Cyclophilin Gene of Toxoplasma Gondii", CHINESE JOURNAL OF BIOLOGICALS, vol. 23, no. 9, 30 September 2010 (2010-09-30), pages 961 - 966, XP055770951
HUANG, JIN'GUI ET AL.: "Studies on Immuno-Protection from a Toxoplasma Gondii Infection Provided by a TgCyP Subunit Vaccine", JOURNAL OF PATHOGEN BIOLOGY, vol. 6, no. 7, 31 July 2011 (2011-07-31), pages 517 - 520, XP055770979
LI YUNNA, CLONING AND PROKARYOTIC EXPRESSION OF TOXOPLASMA GONDII CYCLOPHILIN GENE, 2010
See also references of EP 3838916A4
Attorney, Agent or Firm:
SBZL IP LAW OFFICE (CN)
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