Title:
VIRUS-LIKE PARTICLE, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/125739
Kind Code:
A1
Abstract:
Provided are a virus-like particle, and a preparation method therefor and the use thereof. In the method, a small ubiquitin-like modifier protein is used as a tag protein to promote the correct folding of virus structural proteins VP0, VP1 and VP3, thereby ensuring the structural stability of the proteins, and achieving the expression of a large amount of structural proteins. After three structural proteins are self-assembled in vitro, a large amount of virus-like particles having similar properties to natural viruses are obtained. By means of immunogenicity detection, the virus-like particles prepared have a relatively high immunogenicity, and can be used as reserve vaccines for the prevention and control of a swine vesicular disease or foot-and-mouth disease virus transmission.
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Inventors:
GUO HUICHEN (CN)
SUN SHIQI (CN)
DONG HU (CN)
MU SUYU (CN)
BAI MANYUAN (CN)
HE RONGZE (CN)
ZHANG YUN (CN)
WU JINEN (CN)
DING YAOZHONG (CN)
FENG XIA (CN)
YIN SHUANGHUI (CN)
SUN SHIQI (CN)
DONG HU (CN)
MU SUYU (CN)
BAI MANYUAN (CN)
HE RONGZE (CN)
ZHANG YUN (CN)
WU JINEN (CN)
DING YAOZHONG (CN)
FENG XIA (CN)
YIN SHUANGHUI (CN)
Application Number:
PCT/CN2022/143081
Publication Date:
July 06, 2023
Filing Date:
December 29, 2022
Export Citation:
Assignee:
LANZHOU VETERINARY RES INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES (CN)
International Classes:
C12N15/62; A61K39/125; A61K39/135; A61K39/39; A61P31/14; C12N15/70
Foreign References:
| CN114317571A | 2022-04-12 | |||
| CN106479986A | 2017-03-08 | |||
| CN101914501A | 2010-12-15 |
Other References:
DATABASE Nucleotide 16 February 2008 (2008-02-16), ANONYMOUS : "Swine vesicular disease virus gene for polyprotein, complete cds ", XP093075028, retrieved from NCBI Database accession no. D16364.1
DATABASE Nucleotide 24 October 2015 (2015-10-24), ANONYMOUS : "Foot-and-mouth disease virus SAT 1 isolate O1K polyprotein gene, partial cds", XP093075055, retrieved from NCBI Database accession no. KR108962.1
DATABASE Nucleotide 15 April 2005 (2005-04-15), ANONYMOUS : "Foot-and-mouth disease virus SAT2 genomic RNA for L, VP4, VP2, VP3, VP1, 2A, 2B, 2C, 3A, VPg1, VPg2, VPg3, pro coding polpolyprotein, strain KEN/3/57", XP093075057, retrieved from NCBI Database accession no. AJ251473.1
DATABASE Nucleotide 17 August 2019 (2019-08-17), ANONYMOUS : "Foot-and-mouth disease virus SAT 3 isolate SAT3/SAR/01/06 polyprotein", XP093075059, retrieved from NCBI Database accession no. MK415736.1
YA-XIA MO, SONG PI, MU SU-YU, DONG HU, CAO SUI-ZHONG: "Prokaryotic expression of capsid protein of Seneca virus type A and assembly of its pentamer", CHINESE VETERINARY SCIENCE, vol. 49, no. 8, 1 January 2019 (2019-01-01), pages 969 - 976, XP093075030, DOI: ：10.16656/j.issn.1673-4696.2019.0098中图分类号：S852.659.6文献标志码：A文章编号：1673-4696(2019)08-0969-08
DATABASE Nucleotide 24 October 2015 (2015-10-24), ANONYMOUS : "Foot-and-mouth disease virus SAT 1 isolate O1K polyprotein gene, partial cds", XP093075055, retrieved from NCBI Database accession no. KR108962.1
DATABASE Nucleotide 15 April 2005 (2005-04-15), ANONYMOUS : "Foot-and-mouth disease virus SAT2 genomic RNA for L, VP4, VP2, VP3, VP1, 2A, 2B, 2C, 3A, VPg1, VPg2, VPg3, pro coding polpolyprotein, strain KEN/3/57", XP093075057, retrieved from NCBI Database accession no. AJ251473.1
DATABASE Nucleotide 17 August 2019 (2019-08-17), ANONYMOUS : "Foot-and-mouth disease virus SAT 3 isolate SAT3/SAR/01/06 polyprotein", XP093075059, retrieved from NCBI Database accession no. MK415736.1
YA-XIA MO, SONG PI, MU SU-YU, DONG HU, CAO SUI-ZHONG: "Prokaryotic expression of capsid protein of Seneca virus type A and assembly of its pentamer", CHINESE VETERINARY SCIENCE, vol. 49, no. 8, 1 January 2019 (2019-01-01), pages 969 - 976, XP093075030, DOI: ：10.16656/j.issn.1673-4696.2019.0098中图分类号：S852.659.6文献标志码：A文章编号：1673-4696(2019)08-0969-08
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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