Title:
WHOLE GENOME DETECTION METHOD USING WHOLE GENOME AMPLIFICATION OF MERS CORONA VIRUS, AND DIAGNOSTIC KIT
Document Type and Number:
WIPO Patent Application WO/2021/091211
Kind Code:
A1
Abstract:
The present invention relates to a method for obtaining a whole genome sequence for determining a MERS corona virus infection, and a use thereof. A method, a kit and a composition of the present invention comprise 14 universal primer sets capable of amplifying the genomic sequence of a corona virus, thereby enabling convenient and rapid identification of the presence of a target virus in a sample and related diseases. In addition, 99.9% accuracy and versatility are confirmed through whole genome sequencing, and 99.88% variant detection is confirmed, and thus the presence of a MERS corona virus and related diseases can be conveniently and rapidly identified. Furthermore, the present invention can be effectively used in the identification of novel viruses by determining, through whole genome sequencing, the presence of novel MERS corona virus mutants.
Inventors:
KIM SEIL (KR)
Application Number:
PCT/KR2020/015281
Publication Date:
May 14, 2021
Filing Date:
November 04, 2020
Export Citation:
Assignee:
KOREA RES INST STANDARDS & SCI (KR)
International Classes:
C12Q1/70; C12Q1/6869
Foreign References:
| KR20180052317A | 2018-05-18 |
Other References:
KIM YOU-JIN, CHO YONG-JOON, KIM DAE-WON, YANG JEONG-SUN, KIM HAK, PARK SUNGHAN, HAN YOUNG WOO, YUN MI-RAN, LEE HAN SAEM, KIM A-REU: "Complete genome sequence of Middle East respiratory syndrome coronavirus KOR/KNIH/002_05_2015, isolated in South Korea", GENOME ANNOUNCEMENTS, vol. 3, no. 4, 27 August 2015 (2015-08-27), XP055811609, DOI: 10.1128/genomeA.00787-15
DATABASE NUCLEOTIDE 2 September 2015 (2015-09-02), ANONYMOUS: "Middle East respiratory syndrome coronavirus isolate MERS-CoV/KOR/KNIH/002_05_2015, complete genome", XP055811624, retrieved from NCBI Database accession no. KT029139
YUSOF MOHAMMED FAROUK, QUEEN KRISTA, ELTAHIR YASSIR MOHAMMED, PADEN CLINTON R, AL HAMMADI ZULAIKHA MOHAMED ABDEL HAMEED, TAO YING,: "Diversity of Middle East respiratory syndrome coronaviruses in 109 dromedary camels based on full-genome sequencing, Abu Dhabi, United Arab Emirates", EMERGING MICROBES & INFECTIONS, vol. 6, no. 1, 1 January 2017 (2017-01-01), pages 1 - 10, XP055811628, DOI: 10.1038/emi.2017.89
PARK DONGHYUN, HUH HEE JAE, KIM YEON JEONG, SON DAE-SOON, JEON HYO-JEONG, IM EU-HYUN, KIM JONG-WON, LEE NAM YONG, KANG EUN-SUK, KA: "Analysis of intrapatient heterogeneity uncovers the microevolution of Middle East respiratory syndrome coronavirus", MOLECULAR CASE STUDIES, vol. 2, no. 6, 1 November 2016 (2016-11-01), pages a001214, XP055811630, ISSN: 2373-2865, DOI: 10.1101/mcs.a001214
DATABASE NUCLEOTIDE 2 September 2015 (2015-09-02), ANONYMOUS: "Middle East respiratory syndrome coronavirus isolate MERS-CoV/KOR/KNIH/002_05_2015, complete genome", XP055811624, retrieved from NCBI Database accession no. KT029139
YUSOF MOHAMMED FAROUK, QUEEN KRISTA, ELTAHIR YASSIR MOHAMMED, PADEN CLINTON R, AL HAMMADI ZULAIKHA MOHAMED ABDEL HAMEED, TAO YING,: "Diversity of Middle East respiratory syndrome coronaviruses in 109 dromedary camels based on full-genome sequencing, Abu Dhabi, United Arab Emirates", EMERGING MICROBES & INFECTIONS, vol. 6, no. 1, 1 January 2017 (2017-01-01), pages 1 - 10, XP055811628, DOI: 10.1038/emi.2017.89
PARK DONGHYUN, HUH HEE JAE, KIM YEON JEONG, SON DAE-SOON, JEON HYO-JEONG, IM EU-HYUN, KIM JONG-WON, LEE NAM YONG, KANG EUN-SUK, KA: "Analysis of intrapatient heterogeneity uncovers the microevolution of Middle East respiratory syndrome coronavirus", MOLECULAR CASE STUDIES, vol. 2, no. 6, 1 November 2016 (2016-11-01), pages a001214, XP055811630, ISSN: 2373-2865, DOI: 10.1101/mcs.a001214
Attorney, Agent or Firm:
B&IP-JOOWON PATENT AND LAW FIRM (KR)
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