Title:
XYLANASE MUTANT HAVING SPECIFIC ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2024/093141
Kind Code:
A1
Abstract:
A xylanase mutant having a high specific activity. The mutant comprises at least one mutation site of T69A/H, V85D, N88I/M/V/F/H/Y, V108L/M/T/H/I, I112V, H131L/I/W/Y/F, T149V/I/K/L/M/N/R/S/W/Y, D154M/Q/A/I/L/N/S/T/W, N160K/I/T, Q167T, S178A, A179D/M/K/L/Q/R/S/W/E, E185K, K186D and T190D/S. The specific activity of the xylanase mutant is significantly higher than the specific activity of wild-type xylanase, and the xylanase mutant can be widely used in the field of feed.
Inventors:
WU XIUXIU (CN)
LI XINPEI (CN)
CHEN GANG (CN)
BAO KAI (CN)
LI XINPEI (CN)
CHEN GANG (CN)
BAO KAI (CN)
Application Number:
PCT/CN2023/087234
Publication Date:
May 10, 2024
Filing Date:
April 10, 2023
Export Citation:
Assignee:
QINGDAO VLAND BIOTECH GROUP CO LTD (CN)
International Classes:
C12N9/42; A23K20/189; C12N15/56
Foreign References:
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| CN102757947A | 2012-10-31 | |||
| CN115029335A | 2022-09-09 | |||
| CN109750015A | 2019-05-14 | |||
| CN106676086A | 2017-05-17 | |||
| CN104109660A | 2014-10-22 | |||
| CN106834255A | 2017-06-13 |
Other References:
HAN NANYU; MA YU; MU YUELIN; TANG XIANGHUA; LI JUNJUN; HUANG ZUNXI: "Enhancing thermal tolerance of a fungal GH11 xylanase guided by B-factor analysis and multiple sequence alignment", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 131, 4 September 2019 (2019-09-04), US , XP085858876, ISSN: 0141-0229, DOI: 10.1016/j.enzmictec.2019.109422
CHENG YA-SHAN; CHEN CHUN-CHI; HUANG JIAN-WEN; KO TZU-PING; HUANG ZHIYONG; GUO REY-TING: "Improving the catalytic performance of a GH11 xylanase by rational protein engineering", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 99, no. 22, 19 June 2015 (2015-06-19), Berlin/Heidelberg, pages 9503 - 9510, XP035579415, ISSN: 0175-7598, DOI: 10.1007/s00253-015-6712-0
CHENG YA-SHAN; CHEN CHUN-CHI; HUANG JIAN-WEN; KO TZU-PING; HUANG ZHIYONG; GUO REY-TING: "Improving the catalytic performance of a GH11 xylanase by rational protein engineering", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 99, no. 22, 19 June 2015 (2015-06-19), Berlin/Heidelberg, pages 9503 - 9510, XP035579415, ISSN: 0175-7598, DOI: 10.1007/s00253-015-6712-0
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW CO., LTD. (CN)
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