Title:
YARROWIA LIPOLYTICA GENETICALLY ENGINEERED BACTERIUM CAPABLE OF PRODUCING β-ELEMENE AND CONSTRUCTION METHOD THEREFOR, AND METHOD FOR PREPARING β-ELEMENE
Document Type and Number:
WIPO Patent Application WO/2023/174453
Kind Code:
A3
Abstract:
The present application relates to a Yarrowia lipolytica genetically engineered bacterium capable of producing β-elemene and a construction method therefor, and a method for preparing β-elemene. The construction method comprises: integrating the Yarrowia lipolytica endogenous HMGR gene, optimized germacrene A synthase HaGASL101WT412SD433NW436A gene, Yarrowia lipolytica endogenous ERG19 gene, Yarrowia lipolytica endogenous ERG13, and HaGASL101WT412SD433NW436ALERG20 fusion gene of farnesyl diphosphate synthase ERG20 into Yarrowia lipolytica, so as to obtain the Yarrowia lipolytica genetically engineered bacterium capable of producing β-elemene. The method can enable carbon flux to flow into a β-elemene synthesis pathway as much as possible, reducing substrate waste and greatly increasing the yield of β-elemene; and the engineered bacterium has good subculture stability, and can be used for large-scale commercial production.
Inventors:
LIU SHUNCHENG (CN)
YUAN LIJIE (CN)
XU LONGXING (CN)
YUAN LIJIE (CN)
XU LONGXING (CN)
Application Number:
PCT/CN2023/103738
Publication Date:
February 01, 2024
Filing Date:
June 29, 2023
Export Citation:
Assignee:
UNIV NORTH CHINA SCIENCE & TECHNOLOGY (CN)
International Classes:
C12N15/81
Domestic Patent References:
| WO2022222213A1 | 2022-10-27 | |||
| WO2019014310A1 | 2019-01-17 |
Foreign References:
| CN111154665A | 2020-05-15 |
Other References:
GAO YUNYUN: "The Study of Microbial Synthesis of Germacrene a the Precursor of β-elemene", MASTER'S' THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, 15 December 2012 (2012-12-15), CN , XP055979607
WANG, ZHENG: "Efficient Synthesis of Sesquiterpene Gemmaene A in Saccharomyces Cerevisiae", ENGINEERING SCIENCE AND TECHNOLOGY 1, CHINA MASTER'S THESES FULL-TEXT DATABASE, 15 June 2022 (2022-06-15), pages B016 - 596
YE MIN, GAO JIAOQI, ZHOU YONGJIN J.: "Global metabolic rewiring of the nonconventional yeast Ogataea polymorpha for biosynthesis of the sesquiterpenoid β-elemene", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 76, 1 March 2023 (2023-03-01), AMSTERDAM, NL, pages 225 - 231, XP093135899, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2023.02.008
WANG, ZHENG: "Efficient Synthesis of Sesquiterpene Gemmaene A in Saccharomyces Cerevisiae", ENGINEERING SCIENCE AND TECHNOLOGY 1, CHINA MASTER'S THESES FULL-TEXT DATABASE, 15 June 2022 (2022-06-15), pages B016 - 596
YE MIN, GAO JIAOQI, ZHOU YONGJIN J.: "Global metabolic rewiring of the nonconventional yeast Ogataea polymorpha for biosynthesis of the sesquiterpenoid β-elemene", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 76, 1 March 2023 (2023-03-01), AMSTERDAM, NL, pages 225 - 231, XP093135899, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2023.02.008
Attorney, Agent or Firm:
KEYCOM PARTNERS , P.C. (CN)
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