Title:
ＤＮＡメチル化編集用キットおよびＤＮＡメチル化編集方法
Document Type and Number:
Japanese Patent JPWO2017090724
Kind Code:
A1
Abstract:
（１）ヌクレアーゼ活性を有しない不活性化型CRISPR-associated endonuclease Cas9（dCas9）と、タグペプチドがリンカーを挟んで複数つながったタグペプチドアレイとの融合蛋白質、またはそれをコードするRNAもしくはDNA、（２）タグペプチド結合部位と、メチル化酵素もしくは脱メチル化酵素との融合蛋白質、またはそれをコードするRNAもしくはDNA、および（３）メチル化または脱メチル化を所望する部位から１kb以内のDNA配列と相補的な配列を含むガイドRNA（gRNA）、またはそれを発現するDNAを含む、DNAメチル化編集用キット。
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Inventors:
Hatada Deho
Morita Junyo
Takuro Horii
Morita Junyo
Takuro Horii
Application Number:
JP2017552723A
Publication Date:
October 18, 2018
Filing Date:
November 25, 2016
Export Citation:
Assignee:
Gunma University
International Classes:
C12N15/09; C12N15/63
Foreign References:
| WO2014152432A2 | 2014-09-25 |
Other References:
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MAEDER M. L. ET AL.: "Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion pr", NATURE BIOTECHNOLOGY, vol. 31, no. 12, JPN6017001381, 2013, pages 1137 - 1142, XP037163685, ISSN: 0003920830, DOI: 10.1038/nbt.2726
MALI P. ET AL.: "Cas9 as a versatile tool for engineering biology", NATURE METHODS, vol. 10, no. 10, JPN6015022389, 2013, pages 957 - 963, XP002718606, ISSN: 0003920831, DOI: 10.1038/nmeth.2649
山崎大賀 他: "ゲノム編集技術を応用したペリセントロメアへの人為的・配列得的ＤＮＡメチル化誘導", BMB2015講演要旨集, vol. 2015, JPN6017001382, 6 November 2015 (2015-11-06), pages 1 - 0832, ISSN: 0003920832
SHEN L. ET AL.: "A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chroma", NUCLEIC ACIDS RESEARCH, vol. 38, no. 18, JPN6017001383, 2010, pages 6054 - 6064, XP055496863, ISSN: 0003920833, DOI: 10.1093/nar/gkq456
CABANTOUS S. ET AL.: "A new protein-protein interaction sensor based on tripartite split-GFP association", SCIENTIFIC REPORTS, vol. 3, JPN6017001384, 2013, pages 2854 - 1, ISSN: 0003920834
SKELTON N. J. ET AL.: "Origins of PDZ domain ligand specificity", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 9, JPN6017001385, 2003, pages 7645 - 7654, XP002454235, ISSN: 0003920835, DOI: 10.1074/jbc.M209751200
MAEDER M. L. ET AL.: "Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion pr", NATURE BIOTECHNOLOGY, vol. 31, no. 12, JPN6017001381, 2013, pages 1137 - 1142, XP037163685, ISSN: 0003920830, DOI: 10.1038/nbt.2726
MALI P. ET AL.: "Cas9 as a versatile tool for engineering biology", NATURE METHODS, vol. 10, no. 10, JPN6015022389, 2013, pages 957 - 963, XP002718606, ISSN: 0003920831, DOI: 10.1038/nmeth.2649
山崎大賀 他: "ゲノム編集技術を応用したペリセントロメアへの人為的・配列得的ＤＮＡメチル化誘導", BMB2015講演要旨集, vol. 2015, JPN6017001382, 6 November 2015 (2015-11-06), pages 1 - 0832, ISSN: 0003920832
SHEN L. ET AL.: "A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chroma", NUCLEIC ACIDS RESEARCH, vol. 38, no. 18, JPN6017001383, 2010, pages 6054 - 6064, XP055496863, ISSN: 0003920833, DOI: 10.1093/nar/gkq456
CABANTOUS S. ET AL.: "A new protein-protein interaction sensor based on tripartite split-GFP association", SCIENTIFIC REPORTS, vol. 3, JPN6017001384, 2013, pages 2854 - 1, ISSN: 0003920834
SKELTON N. J. ET AL.: "Origins of PDZ domain ligand specificity", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 9, JPN6017001385, 2003, pages 7645 - 7654, XP002454235, ISSN: 0003920835, DOI: 10.1074/jbc.M209751200
Attorney, Agent or Firm:
Shinichi Sanuki
Yoshiyuki Kawaguchi
Yoshiyuki Kawaguchi
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