Title:
GENE EXPRESSION ANALYSIS METHOD USING TWO-DIMENSIONAL CDNA LIBRARY
Document Type and Number:
Japanese Patent JP2016052320
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method and/or means for picking out an individual cell to analyze it, and at the same time, quantitatively monitoring expression levels of various genes in the form of maintaining two-dimensional information of a tissue.SOLUTION: The invention provides a method for obtaining gene expression levels in any site or all of the target sites at one cell level positional resolution by producing a nucleic acid library from a genome, ncRNA, or protein in the form of maintaining two-dimensional cell distribution information in a living tissue. More specifically, a sheet shape nucleic acid library is produced from a genome, ncRNA, or protein in the form of maintaining two-dimensional cell distribution information in the living tissue and this is repeatedly used in gene expression detection steps, thereby enabling an expression distribution measurement with high precision for a large number of genes.SELECTED DRAWING: Figure 1
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Inventors:
SHIRAI MASATAKA
KANBARA HIDEKI
TANIGUCHI KIYOMI
KANBARA HIDEKI
TANIGUCHI KIYOMI
Application Number:
JP2015225641A
Publication Date:
April 14, 2016
Filing Date:
November 18, 2015
Export Citation:
Assignee:
HITACHI LTD
International Classes:
C12M1/00; C12M1/34; G01N21/76; G01N21/78; G01N33/53; G01N33/543; G01N33/544
Domestic Patent References:
| JP5878154B2 | 2016-03-08 | |||
| JP2007014297A | 2007-01-25 | |||
| JP2003526331A | 2003-09-09 | |||
| JP2003505694A | 2003-02-12 |
Foreign References:
| WO2011068088A1 | 2011-06-09 | |||
| WO2001007915A2 | 2001-02-01 | |||
| WO2008108004A1 | 2008-09-12 |
Other References:
JPN6016039576; Ｍ． Ｊａｒｖｉｕｓ， ｅｔ ａｌ．， Ｍｏｌ Ｃｅｌｌ Ｐｒｏｔｅｏｍｉｃｓ， ２００７， ６（９）， ｐｐ１５００-１５０９
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Satoshi Fujita