Title:
HOMOGENEOUS ANTIBODY POPULATION
Document Type and Number:
Japanese Patent JP2016145259
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide homogeneous antibody populations.SOLUTION: The present invention is generally directed to methods of producing an increase in the enrichment and/or recovery of preferred forms of monoclonal antibodies. More particularly, the invention relates to methods for eliminating disulfide heterogeneity in the hinge region of recombinant IgG2 antibody proteins. A monoclonal IgG2 antibody is provided, comprising: a light chain polypeptide; and a heavy chain polypeptide having a hinge region, where the antibody comprises an amino acid modification in the heavy or light chain polypeptide such that the modification provides a light chain polypeptide that primarily forms an interchain disulfide bond with amino acids in the hinge region of the heavy chain polypeptide through the most C-terminal cysteine residue of the light chain polypeptide.SELECTED DRAWING: Figure 1B
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Inventors:
THOMAS M DILLON
PAVEL BONDARENKO
JETTE WYPYCH
MARTIN ALLEN
ALAIN BALLAND
RICCI MARGARET SPEED
AMY GUO
SHEN WENYAN
SUN JEONGHOON
CHRIS VEZINA
PAVEL BONDARENKO
JETTE WYPYCH
MARTIN ALLEN
ALAIN BALLAND
RICCI MARGARET SPEED
AMY GUO
SHEN WENYAN
SUN JEONGHOON
CHRIS VEZINA
Application Number:
JP2016098451A
Publication Date:
August 12, 2016
Filing Date:
May 17, 2016
Export Citation:
Assignee:
AMGEN
International Classes:
C07K16/18; A61K39/395; A61P43/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/00; C12N15/02; C12N15/09; C12P21/08
Domestic Patent References:
| JP2007501013A | 2007-01-25 | |||
| JPH0644323A | 1994-02-18 | |||
| JP2001521372A | 2001-11-06 |
Foreign References:
| WO2006047340A2 | 2006-05-04 | |||
| US20060134105A1 | 2006-06-22 | |||
| WO2005003155A1 | 2005-01-13 | |||
| WO2005081898A2 | 2005-09-09 |
Other References:
ANTICANCER RESEACH, vol. Vol.19, JPN6014036104, 1999, pages 13 - 22
PROTEIN ENGINEERING, DESIGN AND SELECTION, vol. 19, no. 7, JPN6015009747, 2006, pages 299 - 307
細胞の分子生物学, vol. 第３版, JPN6013041971, 1995, pages １１２頁
PROTEIN ENGINEERING, DESIGN AND SELECTION, vol. 19, no. 7, JPN6015009747, 2006, pages 299 - 307
細胞の分子生物学, vol. 第３版, JPN6013041971, 1995, pages １１２頁
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita
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