To provide single-chain rare-cutting endonucleases, called meganucleases, which recognize and cleave a specific nucleotide sequence, polynucleotide sequences encoding the rare-cutting endonucleases, a vector comprising the polynucleotide sequences, a cell or non-human animal comprising the polynucleotide sequences or rare-cutting endonucleases, and a process for producing the rare-cutting endonucleases.
The single-chain meganuclease capable of causing DNA cleavage comprises first and second domains in the orientation from N-terminal toward C-terminal, wherein the first and second domains are derived from I-CreI mono-dodecapeptide meganuclease, each of the first and second domains comprises a polypeptide fragment consisting of a dodecapeptide motif and a DNA binding moiety, and the first and second domains are linked via a linker.
CHAMES PATRICK
CHOULIKA ANDRE
EPINAT JEAN-CHARLES
LACROIX EMMANUEL
| JP2006518372A | 2006-08-10 | |||
| JP2006516403A | 2006-07-06 | |||
| JP2007535322A | 2007-12-06 |
JPN6008061933; Ｎａｔ． Ｓｔｒｕｃｔ． Ｂｉｏｌ．， ２００１年， ｖｏｌ． ８， ｎｏ． ４， ｐ． ３１２-３１６
JPN5004012195; ＥＰＩＮＡＴ ＪＥＡＮ-ＣＨＡＲＬＥＳ: ＮＵＣＬＥＩＣ ＡＣＩＤＳ ＲＥＳＥＡＲＣＨ． Ｖ３１ Ｎ１１, 20030601, Ｐ２９５２-２９６２
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