Title:
【発明の名称】相同組換えに効果的なDNA構築物及びその利用
Document Type and Number:
Japanese Patent JPH10500570
Kind Code:
A
Abstract:
The invention relates to constructs comprising: a) a targeting sequence; b) a regulatory sequence; c) an exon; and d) an unpaired splice-donor site. The invention further relates to a method of producing protein in vitro or in vivo comprising the homologous recombination of a construct as described above within a cell. The homologously recombinant cell is then maintained under conditions which will permit transcription and translation, resulting in protein expression. The present invention further relates to homologously recombinant cells, including primary, secondary, or immortalized vertebrate cells, methods of making the cells, methods of homologous recombination to produce fusion genes, methods of altering gene expression in the cells, and methods of making a protein in a cell employing the constructs of the invention.
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Inventors:
Treco, Douglas A.
Hartrain, Michael W.
Selden, Richard F.
Hartrain, Michael W.
Selden, Richard F.
Application Number:
JP52982695A
Publication Date:
January 20, 1998
Filing Date:
May 11, 1995
Export Citation:
Assignee:
Transcaliotherapy, Inc.
International Classes:
A61K31/70; A61K38/00; A61K38/21; A61K38/22; A61K38/27; A61K38/28; A61K38/43; A61K39/395; A61K48/00; A61P43/00; C07H21/04; C07K14/505; C07K14/605; C07K14/61; C12N1/15; C12N5/10; C12N15/09; C12N15/67; C12N15/85; C12N15/90; C12P21/00; C12R1/91; (IPC1-7): C12N15/09; A61K31/70; A61K48/00; C07H21/04; C12N1/15; C12N5/10; C12P21/00
Attorney, Agent or Firm:
Yoshinori Hosoda