Title:
ISOLATED DNA ENCODING FSEI RESTRICTION ENDONUCLEASE AND RELATED ITS PRODUCTION
Document Type and Number:
Japanese Patent JP3939375
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain an isolated DNA encoding FseI restriction endonuclease and capable of producing this enzyme which is one of restriction enzymes and is a tool essential for gene manipulation and is obtained from plasmid pRMFseR 2.
SOLUTION: This new isolated DNA can be obtained from plasmid pRMFseR 2 and encodes FseI restriction endonuclease and is useful for production, or the like, of FseI restriction endonuclease which is one of restriction endonucleases as a tool essential for gene manipulation in modern genetic research. The DNA is obtained by amplifying a gene derived from Frankia- species (NRRL-18528) by using a degenerate primer based on a preserved amino acid sequence of a methylase gene in combination with a degenerate primer based on partial protein sequence data of endonuclease and cloning the endonuclease gene in combination with the methylase gene.
Inventors:
Lichyard Day Morgan
Application Number:
JP27031495A
Publication Date:
July 04, 2007
Filing Date:
October 18, 1995
Export Citation:
Assignee:
New England Bio-Rave's Incorporated
International Classes:
C12N15/09; C07H21/04; C12N1/21; C12N9/10; C12N9/16; C12N9/22; C12N15/55; C12P21/00; C12R1/19; (IPC1-7): C12N15/09; C07H21/04; C12N1/21; C12N9/16; //(C12N1/21; C12R1:19); (C12N9/16; C12R1:19)
Domestic Patent References:
| JP6205683A |
Foreign References:
| US5061628 |
Other References:
Nucleic. Acids Res., (1990), 18, [8], p.2061-2064
Attorney, Agent or Firm:
Yoshio Kawaguchi
Toru Nakamura
Takeshi Funayama
Toru Nakamura
Takeshi Funayama