Title:
LECTIN OF LIMULUS POLYPHEMUS AND METHOD FOR SEPARATING AND PURIFYING THE SAME
Document Type and Number:
Japanese Patent JPH04164100
Kind Code:
A
Abstract:
NEW MATERIAL:An N-acetylneuraminic acid-compatible lectin which is lectin, obtained from Limulus polyphemus and reactive with N-acetylneuraminic acid without reacting with N-acetylaminosguar and N-glycolylneuraminic acid.
USE: A reagent for elucidating in vivo distribution of N-acetylneuraminic acid with ultrahigh specificity for the N-acetylneuraminic acid.
PREPARATION: A hemolymph of Limulus polyphemus is subjected to affinity chromatography using an N-acetylneuraminic acid-containing substance (e.g. human erythrocytic membrane sialoglycoprotein) as a ligand and an adsorbed fraction thereof is then separated and fractionated.
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Inventors:
OKUMA SEIICHI
YANAGI TAKAAKI
KUBOTA HAJIME
TSUBOI ISAMI
KIMURA SEIJI
SASAMOTO YASUHIKO
YANAGI TAKAAKI
KUBOTA HAJIME
TSUBOI ISAMI
KIMURA SEIJI
SASAMOTO YASUHIKO
Application Number:
JP28726490A
Publication Date:
June 09, 1992
Filing Date:
October 26, 1990
Export Citation:
Assignee:
TAIYO FISHERY CO LTD
International Classes:
G01N33/579; C07K1/22; C07K14/42; C07K14/435; (IPC1-7): C07K3/20; C07K15/08; G01N33/579
Attorney, Agent or Firm:
Yusuke Hiraki (2 outside)