To provide a manipulation method surely separating a minute object from the tip part of a probe without being influenced by conditions such as the size of the minute object and to provide a manipulation device.

When the minute object 100 is attached to the tip part 10A of the probe 10 and the minute object 100 is arranged in a specified point P2 on a sample S, the outside force is applied to the probe 10 to which the minute object is attached in the vicinity of the point P2 on the sample S. Thereby, the minute object attached to the tip part 10A of the probe 10 can surely be separated.