Title:
METHOD FOR DETERMINING BOUNDARIES OF Z-STACK OF IMAGES OF OBJECT, CORRESPONDING OPTICAL APPARATUS, AND COMPUTER PROGRAM THEREFOR
Document Type and Number:
Japanese Patent JP2023088309
Kind Code:
A
Abstract:
To provide a method of automatically determining boundaries of a z-stack of images of an object.SOLUTION: The z-stack of the images is acquired by imaging the object at different focal positions by an optical apparatus. The optical apparatus includes an apparatus optical system and a focus adjusting unit for imaging the object at different focal positions through the apparatus optical system. The method includes the steps of: generating a set of images of the object, each image being captured at different focal positions; applying a blurriness-W distance function to each image of the set of images, calculating a distance value for each image by the blurriness-W distance function, and associating respectively different distance values with different image sharpness or blurriness of the corresponding image; and determining the z-stack boundaries according to the focal positions allocated to two secondary extrema when the blurriness-W distance function having the focal position as a variable shows a primary extremum and the two secondary extrema adjacent to the primary extremum.SELECTED DRAWING: Figure 1
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Inventors:
JOSE MIGUEL SERRA LLETI
TOBIAS HESSLER
TOBIAS HESSLER
Application Number:
JP2022198431A
Publication Date:
June 26, 2023
Filing Date:
December 13, 2022
Export Citation:
Assignee:
LEICA MICROSYSTEMS
International Classes:
G02B21/00; G02B7/28; G02B7/36; G02B21/26; G02B21/36
Attorney, Agent or Firm:
Einzel Felix-Reinhard
Taku Morita
Junichi Maekawa
Hideo Nagashima
Ueshima class
Taku Morita
Junichi Maekawa
Hideo Nagashima
Ueshima class