To provide a filtration method for separating a solution containing a useful protein from a culture broth containing the useful protein in tangential flow filtration under perfusion culture, without losing the permeability of the useful protein.
There provided is a filtration method for separating a solution containing a useful protein from a culture broth containing the useful protein which satisfies the following formula (1): X2≥0.9×X1, where under perfusion culture, the flow rate of the permeate liquid from a filtration film is monitored and measured, and when the flow rate of the permeate liquid reaches at least 10% or more of the flow rate of the permeate liquid at the start of the tangential flow filtration, including the process of the replacement of the filtration film, the permeability of the protein in the permeate liquid 1 minute after the start of the filtration is X1, and the permeability of the protein in the permeate liquid at the time of the replacement of the filtration film is X2.
KATO CHIHIRO
MATSUNO TETSUGAN
| JPS61257181A | 1986-11-14 | |||
| JP2011212608A | 2011-10-27 | |||
| JP2007245084A | 2007-09-27 | |||
| JP2012006864A | 2012-01-12 | |||
| JPS63319004A | 1988-12-27 | |||
| JPH02127197U | 1990-10-19 |
| WO2011001963A1 | 2011-01-06 |
Toshifumi Onuki
Akihiko Eguchi
Kazuhiko Naito