METHOD FOR PRODUCING AND TRANSFORMING CASSAVA PROTOPLASTS

Title:

METHOD FOR PRODUCING AND TRANSFORMING CASSAVA PROTOPLASTS

Document Type and Number:

Japanese Patent JP2011135894

Kind Code:

A

Abstract:

To provide a method for producing protoplasts of cassava or closely related species, which protoplasts are capable of regeneration into plants.

The method comprises producing friable embryogenic callus from explants of cassava or closely related species and isolating protoplasts from the friable embryogenic callus. The invention also concerns protoplasts obtainable by the method. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. In addition, the invention concerns a method for regenerating plants from these protoplasts and a cassava plant or closely related species obtainable thereby.

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Inventors:

VISSER RICHARD G F
RAEMAKERS CHRISTIAAN J J
JACOBSON EVERT
BERGERVOET VAN DEELEN JOHANNA ELIZABETH M

Application Number:

JP2011045386A

Publication Date:

July 14, 2011

Filing Date:

March 02, 2011

Export Citation:

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Assignee:

BRUNOB II BV
AVEBE

International Classes:

A01H5/00; C12N15/09; A01H4/00; A01H5/06; A23L1/30; A61K8/00; A61K8/73; A61K8/96; A61K36/47; A61K47/36; A61Q1/00; A61Q1/08; A61Q1/10; A61Q1/12; A61Q5/00; A61Q5/02; A61Q5/06; A61Q5/12; A61Q11/00; A61Q15/00; A61Q19/00; A61Q19/10; C07G99/00; C08B30/00; C08B30/04; C08B30/20; C08L3/02; C08L3/12; C12N5/04; C12N5/10; C12N5/14; C12N15/82; C12P19/04

Domestic Patent References:

JP2000510707A	2000-08-22
JP2003092938A	2003-04-02

Foreign References:

FR1184513A	1959-07-22
WO2000054606A1	2000-09-21

Other References:

JPN6009052115; Ｅｕｐｈｙｔｉｃａｖｏｌ．　９６, 1997, ６５-７５
JPN6009052119; Ｐｌａｎｔ　Ｍｏｌ．　Ｂｉｏｌ．ｖｏｌ．　２３, 1993, ９４７-９６２
JPN6010062862; Ｍｏｌ．　Ｂｒｅｅｄ．ｖｏｌ．　２, 1996, ３３９-３４９
JPN6008013128; Ｅｕｐｈｙｔｉｃａｖｏｌ．　９６, 1997, １５３-１６１
JPN6008013129; Ｐｌａｎｔ　Ｓｃｉ．ｖｏｌ．　１３５, 1998, ８７-１０１

Attorney, Agent or Firm:

Atsushi Aoki
Takashi Ishida
Tetsuji Koga
Nagasaka Tomoyasu
Yoshihiro Kobayashi
Kenichi Kato

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