To provide a microscope control device that can suppress a computation load required for distortion correction processing in a defocus detection processing using a phase-difference optical system, and an optical distortion correction method thereof.
The microscope control device includes an offset processing unit for correcting phase-difference information on a phase-difference between phase-difference images that is generated based on a pair of phase-difference images obtained by imaging a sample by the microscope based on offset information of a phase-difference due to an optical distortion intrinsic to the microscope, and a defocus amount calculation unit for calculating the defocus amount of the sample based on the offset-corrected phase-difference information.
JPH10111459 | MICROSCOPE |
JPH0678881 | APPARATUS FOR OBSERVING AND PHOTOGRAPHING ENDOTHELIAL CELL OF CORNEA |
WO/2020/212480 | FLUORESCENCE MICROSCOPE AND METHOD FOR IMAGING A SAMPLE |
Tetsuo Kanamoto
Koji Hagiwara
Kazuki Matsumoto
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