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Title:
硬い金属イオンのアフィニティークロマトグラフィーを用いた、ペプチド精製
Document Type and Number:
Japanese Patent JP2008507954
Kind Code:
A
Abstract:
A polymer substrate functionalized with a functionality comprising at least one cyclic, metal ion coordinating ligand group which comprises at least 3 nitrogen donor atoms in the ring of the cyclic group, at least one of the nitrogen atoms having an optionally substituted carboxy(lower alkyl) or optionally substituted phosphono(lower alkyl) group covalently attached thereto, is well suited for use in conjunction with "hard" metal ions of low toxicity (such as Ca 2+ , Mg 2+ or Fe 3+ ) in the separation/purification of appropriately "tagged" polypeptides by I mmobilized M etal ion A ffinity C hromatography (IMAC).

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Inventors:
Hearne, Milton Thomas William
Spice, loan
Darry, Rachel
Kureha, Out
Application Number:
JP2007515950A
Publication Date:
March 21, 2008
Filing Date:
June 14, 2005
Export Citation:
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Assignee:
NOVO NORDISK AKTIE SELSXAB
International Classes:
C12N15/09; B01D15/38; B01J20/26; B01J20/281; B01J45/00; C07K1/04; C07K1/22; C07K7/06; C07K7/08; C07K19/00; C08B37/00; C12N1/21; C12N15/63; C12P21/02; G01N30/88
Foreign References:
WO2003042249A22003-05-22
US20040031072A12004-02-12
WO2002082051A22002-10-17
WO1995028966A11995-11-02
Other References:
ACTA RADIOLOGICA, 1997, VOL.SUPPLEMENT 412, P.91-99, JPN6011002067, ISSN: 0001826785
BIOCONJUGATE CHEMISTRY, 2001, VOL.12, NO.2, P.213-219, JPN6011002063, ISSN: 0001826786
APPL. MICROBIOL. BIOTECHNOL., 2003, VOL.63, P.42-50, JPN6011002064, ISSN: 0001826787
ORGANIC LETTERS, 2001, VOL.3, NO.12, P.1877-1879, JPN6011002066, ISSN: 0001826788
Attorney, Agent or Firm:
Takehiko Suzue
Satoshi Kono
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Toshio Shirane
Sadao Muramatsu
Nobuhisa Nogawa
Katsu Sunagawa
Ryo Hashimoto
Tetsuya Kazama



 
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