SUPERCHARGED PROTEINS FOR CELL PENETRATION

Title:

SUPERCHARGED PROTEINS FOR CELL PENETRATION

Document Type and Number:

Japanese Patent JP2014159484

Kind Code:

A

Abstract:

PROBLEM TO BE SOLVED: To provide a supercharged protein for cell penetration.SOLUTION: The invention provides compositions, preparations, systems, and related methods for delivering a supercharged protein, or a complex of a supercharged protein and an agent (e.g., nucleic acids, peptides, proteins, small molecules) to cells. Such systems and methods include the use of supercharged proteins. For example, superpositively charged proteins may be associated with nucleic acids (which typically have a net negative charge) via electrostatic interactions. In some embodiments, such systems and methods comprise modifying a primary sequence of a protein to "supercharge" the protein (for example, to generate a superpositively charged protein). In some embodiments, complexes comprising supercharged proteins and one or more agents to be delivered are useful as therapeutic agents.

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Inventors:

LIU DAVID R
BRIAN R MCNAUGHTON
JAMES JOSEPH CRONICAN
DAVID B THOMPSON

Application Number:

JP2014114885A

Publication Date:

September 04, 2014

Filing Date:

June 03, 2014

Export Citation:

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Assignee:

HARVARD COLLEGE

International Classes:

C07K14/47; A61K31/7088; A61K31/7105; A61K31/711; A61K31/713; A61K35/74; A61K35/76; A61K45/00; A61K47/42; A61K47/48; A61K48/00; A61P31/00; A61P35/00; C12Q1/02; C12N15/09

Foreign References:

WO2007143574A1	2007-12-13
US20040102606A1	2004-05-27
US20070105182A1	2007-05-10
US20030236214A1	2003-12-25
US20030134352A1	2003-07-17

Other References:

JPN6015002267; ＣＭＬＳ，　Ｃｅｌｌ　Ｍｏｌ．　Ｌｉｆｅ　Ｓｃｉ．ｖｏｌ．６２, 2005, ｐｐ．１８３９-１８４９
JPN6015002268; ＭＯＬＥＣＵＬＡＲ　ＴＨＥＲＡＰＹｖｏｌ．５　ｎｏ．２, 2002, ｐｐ．１０４-１１４
JPN6015002269; Ｂｉｏｃｈｅｍｉｓｔｒｙｖｏｌ．３６, 1997, ｐｐ．３００８-３０１７
JPN6015002271; Ｂｉｏｃｈｅｍｉｃａｌ　ａｎｄ　Ｂｉｏｐｈｙｓｉｃａｌ　Ｒｅｓｅａｒｃｈ　Ｃｏｍｍｕｎｉｃａｔｉｏｎｓｖｏｌ．２９１, 2002, ｐｐ．３６７-３７１
JPN6015002272; ＴＨＥ　ＪＯＵＲＮＡＬ　ＯＦ　ＢＩＯＬＯＧＩＣＡＬ　ＣＨＥＭＩＳＴＲＹｖｏｌ．２７５　ｎｏ．４３, 2000, ｐｐ．３３２１３-３３２２１
JPN6015002274; ＪＯＵＲＮＡＬ　ＯＦ　ＶＩＲＯＬＯＧＹｖｏｌ．７９　ｎｏ．１９, 2005, ｐｐ．１２１８５-１２１９８

Attorney, Agent or Firm:

Shusaku Yamamoto
Natsuki Morishita

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