Title:
テロメラーゼ活性測定方法
Document Type and Number:
Japanese Patent JP5888470
Kind Code:
B2
Abstract:
The present disclosure provides a measurement method of telomerase activity with no occurrence of false-negative and high quantitative capability. In the measurement method of telomerase activity of the present disclosure, a solution containing telomerase as a sample solution is mixed with a solution containing primer DNA as a substrate for telomerase even as or after a solution containing non-primer DNA not as a substrate for telomerase is mixed therewith. Non-primer DNA can remove an effect of DNase in the sample solution and prevent occurrence of false-negative. Subsequently, the primer DNA is extended with the telomerase and the extended primer DNA is detected, through which telomerase activity is measured.
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Inventors:
Hidenobu Yaku
Daisuke Miyoshi
Daisuke Miyoshi
Application Number:
JP2015530220A
Publication Date:
March 22, 2016
Filing Date:
August 05, 2014
Export Citation:
Assignee:
Panasonic Corporation
International Classes:
C12Q1/68; C12N15/09; C12Q1/48; G01N27/447
Domestic Patent References:
| JPH09206081A | 1997-08-12 | |||
| JP2012532092A | 2012-12-13 | |||
| JP2004261062A | 2004-09-24 | |||
| JPH09206081A | 1997-08-12 |
Foreign References:
| WO2010116423A1 | 2010-10-14 |
Other References:
JPN6015034938; Ｂｉｏｔｅｃｈｎｉｑｕｅｓ Ｖｏｌ．３５， Ｎｏ．１, 2003, ｐ．１５８-１６２
Attorney, Agent or Firm:
Tokuda Yoshiaki
Koichi Nomura
Koichi Nomura