Title:
【発明の名称】新規なエンドグルカナーゼ
Document Type and Number:
Japanese Patent JP3360830
Kind Code:
B2
Abstract:
An enzyme preparation consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequence Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa Trp Xaa 1 2 3 4 5 6 7 8 9 10 11 12 13 14
and a second amino acid sequence consisting of 5 amino acid residues having the following sequence Trp Cys Cys Xaa Cys 1 2 3 4 5
wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
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Inventors:
Schlein, Martin
Reine Nonvoie, Andersen
Lassen, Soren Frensted
Kaupinen, Marx Sakari
Lange, Reine
Nielsen, Rubuirum
Michiko Ihara
Shinobu Takagi
Reine Nonvoie, Andersen
Lassen, Soren Frensted
Kaupinen, Marx Sakari
Lange, Reine
Nielsen, Rubuirum
Michiko Ihara
Shinobu Takagi
Application Number:
JP52799396A
Publication Date:
January 07, 2003
Filing Date:
March 18, 1996
Export Citation:
Assignee:
Novozymes actiesel scab
International Classes:
C12N15/09; C05F11/08; C05F17/00; C07G99/00; C11D3/386; C12N1/19; C12N9/42; C12N15/00; C12S3/04; C12S11/00; D06M13/02; D06M13/322; D06M13/46; D06M16/00; D06P5/02; D06P5/15; D21C5/00; D21H11/16; D21H11/20; D21H21/10; C12R1/645; C12R1/69; C12R1/77; C12R1/80; D21H17/00; (IPC1-7): C12N15/09; C11D3/386; C12N1/19; C12N9/42; C12S3/04; C12S11/00
Other References:
【文献】国際公開94/7998(WO,A1)
Attorney, Agent or Firm:
Takashi Ishida (8 outside)