ＤＮＡの塩基配列決定方法

Japanese Patent JP3155279

B2

Disclosed is a method for determining a nucleotide sequence of DNA product amplified by polymerase chain reaction not requiring removal of primers and/or 2'-deoxyribonucleoside-5'-triphosphates and/or derivatives thereof, which comprises reacting ribonucleoside-5'-triphosphates comprising ATP, GTP, CTP, UTP and derivatives thereof and one or more of 3'-deoxyribonucleotide-5'-triphosphates comprising 3'-dATP, 3'-dGTP, 3'-dCTP, 3'-dUTP and derivatives thereof in the presence of an RNA polymerase and a DNA product which has been amplified by polymerase chain reaction and contains a promoter sequence for the RNA polymerase to afford a nucleic acid transcription product, separating the obtained nucleic acid transcription product and determining a nucleic acid sequence from the resluting separated fractions.

JP2003139771	METHOD OF SCREENING COMPOUND HAVING ACTION SIMILAR TO THAT OF ESTROGEN
JP2017528715	Systems and methods for determining cell age
JP5801349	A method for identifying the clone source of restricted fragments

佐々木 宣哉
林崎 良英

JP51519496A

April 09, 2001

November 06, 1995

Click for automatic bibliography generation  

理化学研究所

C12Q1/68; (IPC1-7): C12Q1/68

Ｂｉｏｃｈｅｍｉｓｔｒｙ，Ｖｏｌ．２４［２１］（１９８５）ｐ．５７１６−５７２３
ＤＮＡ，Ｖｏｌ．５［２］（１９８６）ｐ．１６７−１７１
Ｇｅｎｅ Ａｎａｌ．Ｔｅｃｈ．Ｖｏｌ．３［４］（１９８６）ｐ．５９−６６
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ，Ｖｏｌ．７５［１１］（１９７８）ｐ．５３３４−５３３８
Ｓｃｉｅｎｃｅ，Ｖｏｌ．２３９（１９８８）ｐ．４９１−４９４

塩澤 寿夫 （外１名）

Previous Patent: オートバイ用シールド

Next Patent: SYSTEM FOR IMPROVING PICTURE QUALITY DETERIORATION DUE TO FOLDED DISTORTION