Title:
ANTIBACTERIAL NANOZYME AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2021/248674
Kind Code:
A1
Abstract:
An antibacterial nanozyme and a preparation method therefor, relating to the technical field of antibacterial material. The preparation method is as follows: 1) heating and stirring an aqueous chloroauric acid solution, adding an aqueous sodium citrate solution, and obtaining a gold seed solution by means of boiling and cooling; 2) blending an aqueous ammonia solution, hemin, hydrazine hydrate and a sonicated aqueous graphene oxide solution, and obtaining a hemin-graphene complex by means of heating for reaction, centrifugation, cleaning and drying; and 3) blending an aqueous hydroxylammonium chloride solution, the gold seed solution obtained in step 1), the hemin-graphene complex obtained in step 2) and an aqueous chloroauric acid solution, stirring until the solution becomes cyan, stirring the solution again, stopping stirring and allowing the solution to stand to obtain the nanozyme. The photothermal performance exhibited by the nanozyme under 808 nm near-infrared laser irradiation and active oxygen free radicals generated on the surface of the nanozyme achieve high antibacterial efficacy.
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Inventors:
ZHOU HONG (CN)
LIU JING (CN)
LIU JING (CN)
Application Number:
PCT/CN2020/108169
Publication Date:
December 16, 2021
Filing Date:
August 10, 2020
Export Citation:
Assignee:
QINGDAO UNIV OF SCIENCE AND TECHNOLOGY (CN)
International Classes:
A61K41/00; A61P31/04; B22F1/00; B22F9/24; B82Y5/00; B82Y30/00; B82Y40/00; C01B32/184
Foreign References:
| CN111505077A | 2020-08-07 | |||
| CN107314981A | 2017-11-03 | |||
| CN104597240A | 2015-05-06 | |||
| CN103551194A | 2014-02-05 |
Other References:
LIU JING, MEIRONGCUI, LI NIU, HONGZHOU, SHUSHENG ZHANG: "Enhanced Peroxidase-Like Properties of Graphene-Hemin-Composite Decorated with Au Nanoflowers as Electrochemical Aptamer Biosensor for the Detection of K562 Leukemia Cancer Cells", CHEMISTRY-A EUROPEAN JOURNAL, vol. 22, 12 December 2016 (2016-12-12), pages 18001 - 18008, XP055878938, DOI: 10.1002/chem.201604354
LUO CHENG, LI YAN, LONG JIANGANG: "Recent advances in applications of nanoparticles as enzyme mimetics", ZHONGGUO KEXUE. HUAXUE - SCIENTIA SINICA CHIMICA, ZHONGGUO KEXUE ZAZHISHE, CN, vol. 45, no. 10, 1 October 2015 (2015-10-01), CN , pages 1026 - 1041, XP055878713, ISSN: 1674-7224, DOI: 10.1360/N032015-00058
GU CHANG-JIE, KONG FEN-YING, CHEN ZHI-DONG, FAN DA-HE, FANG HAI-LIN, WANG WEI: "Reduced Graphene Oxide-Hemin-Au Nanohybrids: Facile One-Pot Synthesis and Enhanced Electrocatalytic Activity towards the Reduction of Hydrogen Peroxide，", BIOSENSORS AND BIOELECTRONICS, vol. 78, 14 November 2015 (2015-11-14), Amsterdam , NL , pages 300 - 307, XP009532745, ISSN: 0956-5663, DOI: 10.1016/j.bios.2015.11.035
WU ZHIFU, WANG ZUO-LIN: "The Influence of Graphene Oxide Lateral Size and Solution Concentration on Its Antibacterial Effect", JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, vol. 29, no. 1, 28 February 2019 (2019-02-28), pages 23 - 29, XP055878717, ISSN: 1005-4979, DOI: 10.3969/j.issn.1005-4979.2019.01.004
DONG HAIJIAO, ZHANG CHI;FAN YAO-YAO;ZHANG WEI;GU NING;ZHANG YU: "Nanozyme and Their ROS Regulation Effect in Cells", PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, vol. 45, no. 2, 31 December 2018 (2018-12-31), pages 105 - 117, XP055878722, DOI: 10.16476/j.pibb.2017.0460
LI JIANG, HAN SHUAI, WANG CHAO, SHI CHENG-CHENG, YIN YAO-BING: "Preparation of Hemin-NH 2 -Graphene and Its Dye Degradation Performance", HUAXUE SHIJI - CHEMICAL REAGENTS, BEIJING : HUAXUE HUAXUE SHIJI KEJI QINGBAO ZHONGXINZHAN, CN, vol. 42, no. 6, 20 April 2020 (2020-04-20), CN , pages 617 - 621, XP055878724, ISSN: 0258-3283, DOI: 10.13822/j.cnki.hxsj.2020007448
HAN XIAOBO, ZHENG YINGHONG;YANG LIMING: "Advancement of Photosensitizers for Photodynamic Therapy", JOURNAL OF SHANGHAI UNIVERSITY (NATURAL SCIENCE EDITION), vol. 23, no. 2, 30 April 2017 (2017-04-30), pages 169 - 178, XP055878726, ISSN: 1007-2861, DOI: 10.3969/j.issn.1007-2861.2017.01.007
LUO CHENG, LI YAN, LONG JIANGANG: "Recent advances in applications of nanoparticles as enzyme mimetics", ZHONGGUO KEXUE. HUAXUE - SCIENTIA SINICA CHIMICA, ZHONGGUO KEXUE ZAZHISHE, CN, vol. 45, no. 10, 1 October 2015 (2015-10-01), CN , pages 1026 - 1041, XP055878713, ISSN: 1674-7224, DOI: 10.1360/N032015-00058
GU CHANG-JIE, KONG FEN-YING, CHEN ZHI-DONG, FAN DA-HE, FANG HAI-LIN, WANG WEI: "Reduced Graphene Oxide-Hemin-Au Nanohybrids: Facile One-Pot Synthesis and Enhanced Electrocatalytic Activity towards the Reduction of Hydrogen Peroxide，", BIOSENSORS AND BIOELECTRONICS, vol. 78, 14 November 2015 (2015-11-14), Amsterdam , NL , pages 300 - 307, XP009532745, ISSN: 0956-5663, DOI: 10.1016/j.bios.2015.11.035
WU ZHIFU, WANG ZUO-LIN: "The Influence of Graphene Oxide Lateral Size and Solution Concentration on Its Antibacterial Effect", JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, vol. 29, no. 1, 28 February 2019 (2019-02-28), pages 23 - 29, XP055878717, ISSN: 1005-4979, DOI: 10.3969/j.issn.1005-4979.2019.01.004
DONG HAIJIAO, ZHANG CHI;FAN YAO-YAO;ZHANG WEI;GU NING;ZHANG YU: "Nanozyme and Their ROS Regulation Effect in Cells", PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, vol. 45, no. 2, 31 December 2018 (2018-12-31), pages 105 - 117, XP055878722, DOI: 10.16476/j.pibb.2017.0460
LI JIANG, HAN SHUAI, WANG CHAO, SHI CHENG-CHENG, YIN YAO-BING: "Preparation of Hemin-NH 2 -Graphene and Its Dye Degradation Performance", HUAXUE SHIJI - CHEMICAL REAGENTS, BEIJING : HUAXUE HUAXUE SHIJI KEJI QINGBAO ZHONGXINZHAN, CN, vol. 42, no. 6, 20 April 2020 (2020-04-20), CN , pages 617 - 621, XP055878724, ISSN: 0258-3283, DOI: 10.13822/j.cnki.hxsj.2020007448
HAN XIAOBO, ZHENG YINGHONG;YANG LIMING: "Advancement of Photosensitizers for Photodynamic Therapy", JOURNAL OF SHANGHAI UNIVERSITY (NATURAL SCIENCE EDITION), vol. 23, no. 2, 30 April 2017 (2017-04-30), pages 169 - 178, XP055878726, ISSN: 1007-2861, DOI: 10.3969/j.issn.1007-2861.2017.01.007
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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