Title:
CANCER CELL DETERMINATION METHOD USING FOXB2 GENE
Document Type and Number:
WIPO Patent Application WO/2017/142070
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method that enables easy determination of cancer cells. The present invention relates to a cancer cell determination method comprising the following steps 1-3: (1) a step 1 for subjecting a cell-derived nucleic acid to a bisulfite reaction, said nucleic acid at least including a base sequence represented by SEQ ID NO: 2 and including some or all of a base sequence represented by SEQ ID NO: 1; (2) a step 2 for amplifying, among the reaction products obtained at step 1, a bisulfite reaction product of a nucleic acid at least including a base sequence represented by SEQ ID NO: 2 and comprising some or all of a base sequence represented by SEQ ID NO: 1; and (3) a step 3 for determining whether said cells are cancer cells on the basis of the result of subjecting the amplified product obtained at step 2 to electrophoresis. The present invention also relates to a primer set comprising: a forward primer comprising a base sequence represented by SEQ ID NO: 6; and a reverse primer comprising some or all of SEQ ID NO: 8 and comprising a base sequence that at least includes a base sequence represented by SEQ ID NO: 7, or a reverse primer comprising a base sequence represented by SEQ ID NO: 9. The present invention further relates to a reagent for cancer cell determination, said reagent including said primer set.
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Inventors:
HAYASHIDA YUKINOBU (JP)
Application Number:
PCT/JP2017/005947
Publication Date:
August 24, 2017
Filing Date:
February 17, 2017
Export Citation:
Assignee:
WAKO PURE CHEM IND LTD (JP)
International Classes:
C12N15/09; C12Q1/68; G01N33/53; G01N33/574
Domestic Patent References:
| WO2013089063A1 | 2013-06-20 |
Foreign References:
| JP2008283947A | 2008-11-27 |
Other References:
VAN ROON EH ET AL.: "BRAF mutation-specific promoter methylation of FOX genes in colorectal cancer", CLIN. EPIGENETICS, vol. 5, no. 2, 2013, pages 1 - 10, XP055540765
KEITA M ET AL.: "Global methylation profiling in serous ovarian cancer is indicative for distinct aberrant DNA methylation signatures associated with tumor aggressiveness and disease progression", GYNECOL. ONCOL., vol. 128, no. 2, 2013, pages 356 - 363, XP055080828
YUKINOBU HAYASHIDA: "Kokando Bisulfite-ho no Kaihatsu = [Development of high sensitivity Bisulfite method]", WAKO JUN'YAKU JIHO, vol. 80, no. 2, 2012, pages 10 - 11, XP009513183, ISSN: 1347-4804
See also references of EP 3418392A4
KEITA M ET AL.: "Global methylation profiling in serous ovarian cancer is indicative for distinct aberrant DNA methylation signatures associated with tumor aggressiveness and disease progression", GYNECOL. ONCOL., vol. 128, no. 2, 2013, pages 356 - 363, XP055080828
YUKINOBU HAYASHIDA: "Kokando Bisulfite-ho no Kaihatsu = [Development of high sensitivity Bisulfite method]", WAKO JUN'YAKU JIHO, vol. 80, no. 2, 2012, pages 10 - 11, XP009513183, ISSN: 1347-4804
See also references of EP 3418392A4
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