Title:
COMPLEMENT ACTIVATED C1S ENZYME FLUORESCENCE DETECTION KIT, DETECTION METHOD, AND USE
Document Type and Number:
WIPO Patent Application WO/2023/221325
Kind Code:
A1
Abstract:
The present invention relates to the field of biotechnology, and specifically relates to a complement activated C1s enzyme fluorescence detection kit, a detection method, and the use. According to the present invention, a complement activated C1s enzyme fluorescence detection kit, a detection method, and the use are established for the purpose of detecting the enzyme activity level of complement activated C1s in a sample. In the present invention, on the basis of magnetic beads precoated with a recombinant C1s antibody, C1s in a C1s standard or in a sample to be detected is specifically captured; magnetic separation is performed to remove a non-specific conjugate, an activated C1s enzyme digested substrate peptide labeled with a FRET fluorescent substance is added, and then a fluorescence signal in a reaction system is detected by using a microplate reader; quantitative detection is performed on the enzyme activity of the activated C1s by detecting the change of fluorescence intensity. The present invention has the characteristics of high sensitivity, strong specificity, small error, simple and convenient operation, etc. The enzyme activity after activation of C1s in human or animal blood and body fluids can be detected so as to accurately evaluate the classical activation state of the complement in the body.
Inventors:
XIA SHENG (CN)
YE JUN (CN)
YE JUN (CN)
Application Number:
PCT/CN2022/115117
Publication Date:
November 23, 2023
Filing Date:
August 26, 2022
Export Citation:
Assignee:
UNIV JIANGSU (CN)
International Classes:
C07K16/40; G01N33/543; G01N33/573; G01N33/58; G01N33/68
Domestic Patent References:
| WO2014071206A1 | 2014-05-08 |
Foreign References:
| CN110753701A | 2020-02-04 | |||
| CN114874329A | 2022-08-09 | |||
| US20070254322A1 | 2007-11-01 | |||
| US4693970A | 1987-09-15 | |||
| GB201403961D0 | 2014-04-23 | |||
| US20220056117A1 | 2022-02-24 | |||
| US20100291106A1 | 2010-11-18 |
Attorney, Agent or Firm:
NANJING DOYES INTELLECTUAL PROPERTY CO., LTD. (CN)
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