Title:
ENGINEERED STRAIN OF YEAST HAVING MITOCHONDRION-POSITIONED REDUCTIVE TCA PATHWAY AND EFFICIENTLY PRODUCING SUCCINIC ACID, CONSTRUCTION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/197692
Kind Code:
A1
Abstract:
The present invention provides an engineered strain of yeast having mitochondrion-positioned reductive TCA pathway and efficiently producing succinic acid, a construction method therefor and use thereof, and belongs to the technical field of microorganisms and fermentation engineering. According to the present invention, the enzymes related to the biosynthesis pathway of succinic acid in yeast cells are over-expressed and positioned to the mitochondrial matrix to facilitate the functional expression of the reductive TCA pathway in eukaryotic microorganisms. The strain can utilize common carbon and nitrogen sources, and after fed-batch fermentation culture, the succinic acid yield reaches 50.1 g/L, and the conversion rate reaches 0.75 g/g of glucose. As an excellent strain for efficiently producing succinic acid, the strain has good application prospects in the fields of food, chemical engineering, degradable materials, and the like.
Inventors:
MIAO HANGEN (CN)
TANG JINKUI (CN)
QI QINGSHENG (CN)
CUI ZHIYONG (CN)
HOU JIN (CN)
ZHONG YUTAO (CN)
DENG JINGYU (CN)
TANG JINKUI (CN)
QI QINGSHENG (CN)
CUI ZHIYONG (CN)
HOU JIN (CN)
ZHONG YUTAO (CN)
DENG JINGYU (CN)
Application Number:
PCT/CN2022/143484
Publication Date:
October 19, 2023
Filing Date:
December 29, 2022
Export Citation:
Assignee:
SHENGHONG HOLDING GROUP CO LTD (CN)
International Classes:
C12N1/19; A23L29/00; C12N15/31; C12N15/53; C12N15/62; C12N15/81; C12P7/46; C12R1/645
Foreign References:
| CN114806913A | 2022-07-29 | |||
| CN104818224A | 2015-08-05 | |||
| US20150010973A1 | 2015-01-08 |
Other References:
HUIHUA XIA, YIN GUOMIN, ZHU NIANQING, CHEN TAO: "Effect of Overexpression of Succinate Exporter Suc E on Anaerobic Succinate Production by Corynebacterium glutamicum", CHEMICAL INDUSTRY AND ENGINEERING, vol. 32, no. 2, 15 March 2015 (2015-03-15), pages 74 - 79, XP093099643
PELEG Y, ET AL: "Inducible overexpression of the FUM1 gene in Saccharomyces cerevisiae: Localization of fumarase and efficient fumaric acid bioconversion to L-malic acid", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 56, 1 January 1990 (1990-01-01), US , pages 2777 - 2783, XP002408560, ISSN: 0099-2240
PELEG Y, ET AL: "Inducible overexpression of the FUM1 gene in Saccharomyces cerevisiae: Localization of fumarase and efficient fumaric acid bioconversion to L-malic acid", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 56, 1 January 1990 (1990-01-01), US , pages 2777 - 2783, XP002408560, ISSN: 0099-2240
Attorney, Agent or Firm:
JINAN SHENGDA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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