Title:
FILAMENTOUS FUNGUS FOR USE IN GENE TRANSFER
Document Type and Number:
WIPO Patent Application WO/2023/228969
Kind Code:
A1
Abstract:
The purpose of the present invention is to establish a novel gene transfer system for easily achieving the expression of a heterologous protein and the production of a specific metabolite in a filamentous fungus. A filamentous fungus in which a megaendonuclease recognition sequence is intruded into a retrotransposon in a filamentous fungus is developed, and a gene transfer system in which a desired expression cassette can be introduced easily into a plurality of introduction sites is established by using the filamentous fungus. As a result, a filamentous fungus is provided, in which a nuclease recognition sequence is introduced into each of nucleotide sequences for two or more copies of a moving genetic factor occurring in the genome.
More Like This:
Inventors:
HARA SEIICHI (JP)
Application Number:
PCT/JP2023/019297
Publication Date:
November 30, 2023
Filing Date:
May 24, 2023
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N1/15; C12N15/63
Domestic Patent References:
| WO2019240243A1 | 2019-12-19 |
Foreign References:
| JP2014513535A | 2014-06-05 | |||
| JP2018191561A | 2018-12-06 | |||
| JP2018068292A | 2018-05-10 | |||
| JP2018064484A | 2018-04-26 |
Other References:
MIKI HANASAKI, HIROSHI MASUMOTO: "CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner", SCIENTIFIC REPORTS, vol. 9, no. 1, 1 December 2019 (2019-12-01), pages 15300, XP055660238, DOI: 10.1038/s41598-019-51891-6
OGASAWARA HIRONOBU: "Novel Tranposon in Aspergillus oryzae and Exploiting the Transposability to Industrial Applications", AKITA RESEARCH INSTITUTE OF FOOD AND BREWING, vol. 105, no. 6, 1 January 2010 (2010-01-01), pages 334 - 342, XP093111275
JIE JIN FENG, HARA SEIICHI, SATO ATSUSHI, KOYAMA YASUJI: "Discovery and analysis of an active long terminal repeat-retrotransposable element in Aspergillus oryzae", THE JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, MICROBIOLOGY RESEARCH FOUNDATION, JP, vol. 60, no. 1, 1 January 2014 (2014-01-01), JP , pages 1 - 6, XP093111285, ISSN: 0022-1260, DOI: 10.2323/jgam.60.1
OGASAWARA HIRONOBU: "Novel Tranposon in Aspergillus oryzae and Exploiting the Transposability to Industrial Applications", AKITA RESEARCH INSTITUTE OF FOOD AND BREWING, vol. 105, no. 6, 1 January 2010 (2010-01-01), pages 334 - 342, XP093111275
JIE JIN FENG, HARA SEIICHI, SATO ATSUSHI, KOYAMA YASUJI: "Discovery and analysis of an active long terminal repeat-retrotransposable element in Aspergillus oryzae", THE JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, MICROBIOLOGY RESEARCH FOUNDATION, JP, vol. 60, no. 1, 1 January 2014 (2014-01-01), JP , pages 1 - 6, XP093111285, ISSN: 0022-1260, DOI: 10.2323/jgam.60.1
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Download PDF:
Previous Patent: POLYNUCLEOTIDE
Next Patent: RESIN COMPOSITION, MULTILAYERED STRUCTURE, MOLDED ARTICLE, AND METHOD FOR PRODUCING RESIN COMPOSITIO...
Next Patent: RESIN COMPOSITION, MULTILAYERED STRUCTURE, MOLDED ARTICLE, AND METHOD FOR PRODUCING RESIN COMPOSITIO...