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Title:
FLUORESCENT MICROSCOPIC IMAGING METHOD AND APPARATUS
Document Type and Number:
WIPO Patent Application WO/2017/000701
Kind Code:
A1
Abstract:
A fluorescent microscopic imaging method and apparatus, comprising: according to experiment requirements, illuminating at least one monochromatic fluorescence excitation light source (101) of the same colour from amongst a plurality of monochromatic fluorescence excitation light sources (101) as a target light source, the monochromatic fluorescence excitation light emitted by each monochromatic fluorescence excitation light source (101) being obliquely emitted onto a preset detection area of a sample plate to be detected; on the side of the sample plate to be detected furthest from the target light source, collecting the fluorescence of the particles in the preset detection area excited by the irradiation of the monochromatic fluorescence excitation light emitted by the target light source, and amplifying the preset detection area to a preset multiple; implementing light-filtering processing of the excited fluorescence of the particles in the preset detection area; and acquiring a fluorescent image of the preset detection area. A two-colour mirror is not required, and the fluorescence microscope apparatus therefore has a simple structure and low cost, preventing light energy loss. As the monochromatic fluorescence excitation light is obliquely emitted onto the sample plate to be detected, a small amount of monochromatic fluorescence excitation light is emitted into an objective lens after passing through the sample plate to be detected.

Inventors:
LUO PUWEN (CN)
XIA HAOHAN (CN)
Application Number:
PCT/CN2016/082768
Publication Date:
January 05, 2017
Filing Date:
May 20, 2016
Export Citation:
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Assignee:
SHANGHAI RUIYU BIOTECH CO LTD (CN)
International Classes:
G01N21/64
Foreign References:
CN105092550A2015-11-25
CN205091263U2016-03-16
CN105158220A2015-12-16
CN104990907A2015-10-21
CN102305778A2012-01-04
CN102370462A2012-03-14
CN202057599U2011-11-30
CN204330595U2015-05-13
US5187572A1993-02-16
Other References:
See also references of EP 3318865A4
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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