Title:
GASTRIC CANCER MONITORING KIT AND USE METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2018/157832
Kind Code:
A1
Abstract:
Provided by the present invention are a gastric cancer monitoring kit and a use method therefor, the kit comprising: a reagent A, which is prepared by adding 5% of SDS to an ammonium bicarbonate solution having a concentration of 10 mM; a reagent B, which is prepared by adding no less than 2.4 unit/μL of exoglycosidase to NP40 having a concentration of 10%; a reagent C, which is a mixture liquid composed of, in equal volume, a solution of 20 mM of APTS dissolved in 1.2 M of citric acid and a solution of 2M of an organic reducing agent dissolved in DMSO; and a reagent D, which comprises 0.5 μL of NH4AC having a concentration of 100 mM and a pH of 5.6, 0.2 μL of sialidase, and 2.50 μL of hydrogen peroxide. The kit, on the basis of a fingerprint spectrum for detecting oligosaccharide chains in blood glycoprotein serving as a diagnosis index, evaluates a gastric cancer patient, so that a tumor stage of the gastric cancer is diagnosed.
Inventors:
CHEN CUI YING (CN)
Application Number:
PCT/CN2018/077714
Publication Date:
September 07, 2018
Filing Date:
March 01, 2018
Export Citation:
Assignee:
JIANGSU XIANSIDA BIOTECHNOLOGY CO LTD (CN)
XIANSIDA NANJING BIOTECHNOLOGY CO LTD (CN)
CHANGZHOU JITAI BIOLOGY TECH CO LTD (CN)
XIANSIDA NANJING BIOTECHNOLOGY CO LTD (CN)
CHANGZHOU JITAI BIOLOGY TECH CO LTD (CN)
International Classes:
G01N33/574; C12Q1/34
Foreign References:
| CN106950380A | 2017-07-14 | |||
| CN102565318A | 2012-07-11 | |||
| CN101779128A | 2010-07-14 | |||
| CN102707062A | 2012-10-03 | |||
| CN105372214A | 2016-03-02 | |||
| CN101576495A | 2009-11-11 | |||
| CN104807998A | 2015-07-29 | |||
| US20110092374A1 | 2011-04-21 |
Other References:
QIN, LIPING: "Correlation Between the Sera N-Glycome Characteristics and the TCM Syndromes in Gastric Cancer", MEDICINE & PUBLIC HEALTH, CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, 15 April 2014 (2014-04-15), pages 19 - 29
ZHAO, YUNPENG ET AL.: "Decreased Core-Fucosylation Contributes to Malignancy in Gastric Cancer", PLOS ONE, vol. 9, no. 4, 14 April 2014 (2014-04-14), pages e94536, XP055538385
XU, XINYUN ET AL.: "Establishment and Evaluation of New N-Glycan Biomarkers for Diagnosis of Gastric Cancer", CURRENT IMMUNOLOGY, vol. 2013, no. 3, May 2013 (2013-05-01), pages 213 - 218, ISSN: 1001-2478
WEIXING ET AL.: "A Relevant Research on Changes in Characteristics of N-Glycan in Gastric Cancer and Its Relationship with TCM Syndromes", CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE, vol. 2014, no. 9, September 2014 (2014-09-01), pages 36 - 39,40, ISSN: 1005-5304
LIU, LONG ET AL.: "The Identification and Characterization of Novel N-Glycan-based Biomarkers in Gastric Cancer", PLOS ONE, vol. 8, no. 10, 17 October 2013 (2013-10-17), pages e77821, XP055538393
CAUEWAERT, N. ET AL.: "Noninvasive Diagnosis of Liver Cirrhosis Using DNA Sequencer-Based Total Serum Protein Glycomics", NATURE MEDICINE, vol. 10, no. 4, 7 March 2004 (2004-03-07), pages 429 - 434, XP055538395
CAUEWAERT, N. ET AL.: "Ultrasensitive Profiling and Sequencing of N-Linked Oligosaccharides Using Standard DNA-Sequencing Equipment", GLYCOBIOLOGY, vol. 11, no. 4, April 2001 (2001-04-01), pages 275 - 281, XP055538397
ZHAO, YUNPENG ET AL.: "Decreased Core-Fucosylation Contributes to Malignancy in Gastric Cancer", PLOS ONE, vol. 9, no. 4, 14 April 2014 (2014-04-14), pages e94536, XP055538385
XU, XINYUN ET AL.: "Establishment and Evaluation of New N-Glycan Biomarkers for Diagnosis of Gastric Cancer", CURRENT IMMUNOLOGY, vol. 2013, no. 3, May 2013 (2013-05-01), pages 213 - 218, ISSN: 1001-2478
WEIXING ET AL.: "A Relevant Research on Changes in Characteristics of N-Glycan in Gastric Cancer and Its Relationship with TCM Syndromes", CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE, vol. 2014, no. 9, September 2014 (2014-09-01), pages 36 - 39,40, ISSN: 1005-5304
LIU, LONG ET AL.: "The Identification and Characterization of Novel N-Glycan-based Biomarkers in Gastric Cancer", PLOS ONE, vol. 8, no. 10, 17 October 2013 (2013-10-17), pages e77821, XP055538393
CAUEWAERT, N. ET AL.: "Noninvasive Diagnosis of Liver Cirrhosis Using DNA Sequencer-Based Total Serum Protein Glycomics", NATURE MEDICINE, vol. 10, no. 4, 7 March 2004 (2004-03-07), pages 429 - 434, XP055538395
CAUEWAERT, N. ET AL.: "Ultrasensitive Profiling and Sequencing of N-Linked Oligosaccharides Using Standard DNA-Sequencing Equipment", GLYCOBIOLOGY, vol. 11, no. 4, April 2001 (2001-04-01), pages 275 - 281, XP055538397
Attorney, Agent or Firm:
JW IP LAW FIRM (CN)
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